Effect of thawing rate and post-thaw culture on the cryopreserved fetal rat islets: Functional and morphological correlation

El-Naggar, M.M.; Al-Mashat, Faisal; Elayat, Ahmed A.; Sibiany, Abdul Rahman M.; Ardawi, M. S. M.; Badawoud, Mohammed H.

doi:10.1016/j.lfs.2005.08.043

Cited by 8 publications

(2 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Santos et al (2008) emphasized that cell damage is not only observed during vitrification, but also during the thawing process, when the cell metabolism is being re-established in the presence of toxic agents such as cryoprotectants. According to El-Naggar et al (2006) the cryopreserved tissue should be thawed quickly in three successive washes, Although one washing is enough for thawing of embryos, isolated or in situ preantral follicles, three washing are recommended to remove traces of cryoprotective agents (Lima-Verde et al 2009). Thus, in this study was performed rapid thawing and three washing in minimal essential medium or minimal essential medium with decreasing concentrations of sucrose (0.50, 0.25, 0.0mol/L).…”

Section: Experiments Imentioning

confidence: 99%

Vitrification of bovine preantral follicles with dimethylsulfoxide and sucrose plus α-tocopherol

et al. 2016

View full text Add to dashboard Cite

RESUMO.-[Vitrificação de folículos pré-antrais bovinos com dimetilxulfoxido e sacarose adicionado de α-tocopherol.] Os objetivos deste estudo foram avaliar a vitrificação de folículos pré-antrais bovinos com dimetilsulfóxido (D) e sacarose (S) adicionando α-tocoferol 5mmol/L (T5) ou 10mmol/L (T10) e, avaliar o aquecimento com meio essencial mínimo (m) com ou sem sacarose (s). Ovários de fêmeas bovinas foram coletados de abatedouro, para o experimento I (n= 66) e II (n= 51). No laboratório fragmentos ovarianos foram distribuídos aleatoriamente para o controle fresco e 8 tratamentos de vitrificação (Controle e Dm; Dms, a DSm; DSms; DST5m; DST5ms; DST10m; DST10ms). Os fragmentos ovarianos foram colocados na solução de vitrificação (5 min) e imersos em nitrogênio líquido (-196°C The objective of this study was to evaluate the vitrification of bovine preantral follicles with dimethylsulfoxide (D) and sucrose (S) plus α-tocopherol 5mmol/L (T5) or 10mmol/L (T10) and, evaluate the thawed with minimal essential medium (m) with or without sucrose (s). Ovaries of cows were collected from slaughterhouse for the experiment I (n=66) and II (n=51). In the laboratory ovarian fragments were randomly assigned either to fresh control and 8 vitrification treatments (Controle and Dm; Dms, DSm; DSms; DST5m; DST5ms; DST10m; DST10ms). Ovarian fragments were placed in vitrification solution (5 min) and immersed in liquid nitrogen (-196°C), after a week, the fragments were thawed and analyzed. In the experiments I, preantral follicles were morphologically observed for histological evaluation, (normal; degenerated and developing of stage). In the experiment II, preantral follicles were mechanically isolated from ovarian tissue and examined with trypan blue, where dead and live corresponded to stained or non-stained. The treatments DSm, DSms and DST10m were effective in preserving the morphology in situ. However, the viability of isolated preantral follicles after vitrification remained high only in treatment DST10m. Thus, DST10m preserves survival rates and morphological integrity during vitrification of bovine preantral follicles. tamento DST10m. Assim, DST10m preservou as taxas de sobrevivência e integridade morfológica durante a vitrificação de folículos pré-antrais bovinos.

show abstract

Section: Experiments Imentioning

confidence: 99%

Vitrification of bovine preantral follicles with dimethylsulfoxide and sucrose plus α-tocopherol

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Then, cell suspensions are thawed directly from −40 to 0 °C by rapid warming. The warming rate related to directly thawing a glass cryovial of HUVECs in a 37 °C water bath from −40 to 0 °C is estimated to be approximately 100 °C/min (rapid warming) according to the HUVEC cryopreservation process frequently conducted in our lab and an average of the reported warming rates in the literature for thawing cryovials of different cell types in a 37 °C water. ,− After thawing, the DMSO is removed from the cells by diluting the suspensions in an isotonic medium at room temperature. We assume that no significant water or CPA movements happen between the thawing to 0 °C and warming to RT as dilution begins.…”

Section: Using the Obtained Parameters To Model Huvec Volume Changesmentioning

confidence: 99%

Temperature Dependence of Membrane Permeability Parameters for Five Cell Types Using Nonideal Thermodynamic Assumptions to Mathematically Model Cryopreservation Protocols

Yadegari,

Gabler Pizarro,

Marquez-Curtis

et al. 2024

J. Phys. Chem. B

View full text Add to dashboard Cite

Cryopreservation is the process of preserving biological matter at subzero temperatures for long-term storage. During cryopreservation, cells are susceptible to various injuries that can be mitigated by controlling the cooling and warming profiles and cryoprotective agent (CPA) addition and removal procedures. Mathematical modeling of the changing cell volume at different temperatures can greatly reduce the experiments needed to optimize cryopreservation protocols. Such mathematical modeling requires as inputs the cell membrane permeabilities to water and CPA and the osmotically inactive fraction of the cell. Since the intra-and extracellular solutions are generally thermodynamically nonideal, our group has been incorporating the osmotic virial equation to model the solution thermodynamics that underlie the cell volume change equations, adding the second and third osmotic virial coefficients of the grouped intracellular solute to the cell osmotic parameters that must be measured. In our previous work, we reported methods to obtain cell osmotic parameters at room temperature by fitting experimental cell volume kinetic data with equations that incorporated nonideal solution thermodynamics assumptions. Since the relevant cell volume excursions occur at different temperatures, the temperature dependence of the osmotic parameters plays an important role. In this work, we present a new two-part fitting method to obtain five cell-type-specific parameters (water permeability, dimethyl sulfoxide permeability, osmotically inactive fraction, and the second and third osmotic virial coefficients of the intracellular solution) from experimental measurements of equilibrium cell volume and cell volume as a function of time at room temperature and 0 °C for five cell types, namely, human umbilical vein endothelial cells (HUVECs), H9c2 rat myoblasts, porcine corneal endothelial cells (PCECs), the Jurkat T-lymphocyte cell line, and human cerebral microvascular endothelial cells (hCMECs/D3 cell line). The fitting method in this work is based on both equilibrium and kinetic cell volume data, enabling us to solve some technical challenges and expand our previously reported measurement technique to 0 °C. Finally, we use the measured parameters to model the cell volume changes for a HUVEC cryopreservation protocol to demonstrate the impact of the nonideal thermodynamic assumptions on predicting the changing cell volume during freezing and thawing.

show abstract

Biobanking: The Future of Cell Preservation Strategies

Baust

Corwin

VanBuskirk

2015

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

With established techniques cryopreservation is often viewed as an "old school" discipline yet modern cryopreservation is undergoing another scientific and technology development growth phase. In this regard, today's cryopreservation processes and cryopreserved products are found at the forefront of research in the areas of discovery science, stem cell research, diagnostic development and personalized medicine. As the utilization of cryopreserved cells continues to increase, the demands placed on the biobanking industry are increasing and evolving at an accelerated rate. No longer are samples providing for high immediate post-thaw viability adequate. Researchers are now requiring samples where not only is there high cell recovery but that the product recovered is physiologically and biochemically identical to its pre-freeze state at the genominic, proteomic, structural, functional and reproductive levels. Given this, biobanks are now facing the challenge of adapting strategies and protocols to address these needs moving forward. Recent studies have shown that the control and direction of the molecular response of cells to cryopreservation significantly impacts final outcome. This chapter provides an overview of the molecular stress responses of cells to cryopreservation, the impact of the apoptotic and necrotic cell death continuum and how studies focused on the targeted modulation of common and/or cell specific responses to freezing temperatures provide a path to improving sample quality and utility. This line of investigation has provided a new direction and molecular-based foundation guiding new research, technology development and procedures. As the use of and the knowledge base surrounding cryopreservation continues to expand, this path will continue to provide for improvements in overall efficacy and outcome.

show abstract

Effect of thawing rate and post-thaw culture on the cryopreserved fetal rat islets: Functional and morphological correlation

Cited by 8 publications

References 51 publications

Vitrification of bovine preantral follicles with dimethylsulfoxide and sucrose plus α-tocopherol

Vitrification of bovine preantral follicles with dimethylsulfoxide and sucrose plus α-tocopherol

Temperature Dependence of Membrane Permeability Parameters for Five Cell Types Using Nonideal Thermodynamic Assumptions to Mathematically Model Cryopreservation Protocols

Biobanking: The Future of Cell Preservation Strategies

Contact Info

Product

Resources

About