2020
DOI: 10.5433/1679-0359.2020v41n5supl1p2209
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Effect of the addition of sodium caseinate on the viability of cryopreserved buffalo semen

Abstract: The use of cooled semen in artificial insemination operations results in higher pregnancy rates than the use of frozen semen. This result seems to be related to the more severe damage triggered by the freezing process than that observed during refrigeration. Due to its ability to bind to sperm-binding proteins and calcium ions, sodium caseinate has been studied as a substance capable of preventing early sperm capacitation, a significant cause of the decreased pregnancy rate resulting from the use of frozen sem… Show more

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(2 citation statements)
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“…In order to solve the obstacles described above and allow a more adequate and safe processing of semen, some alternatives for the substitution of skimmed milk and egg yolk have been reported, among which soy lecithin can be highlighted (Alcay et al, 2017;Mehdipour et al, 2017;Najafi et al, 2017;Araújo Silva et al, 2019), caseinate (Da silva et al, 2020) and low-density lipoproteins (Luna-Orozco et al, 2019). Diluent based on these components have demonstrated a protective effect on sperm cells, a fact that makes them important alternatives for the formulation of seminal extenders (Alcay et al, 2017;Araújo Silva et al, 2019;Da silva et al, 2020).…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…In order to solve the obstacles described above and allow a more adequate and safe processing of semen, some alternatives for the substitution of skimmed milk and egg yolk have been reported, among which soy lecithin can be highlighted (Alcay et al, 2017;Mehdipour et al, 2017;Najafi et al, 2017;Araújo Silva et al, 2019), caseinate (Da silva et al, 2020) and low-density lipoproteins (Luna-Orozco et al, 2019). Diluent based on these components have demonstrated a protective effect on sperm cells, a fact that makes them important alternatives for the formulation of seminal extenders (Alcay et al, 2017;Araújo Silva et al, 2019;Da silva et al, 2020).…”
Section: Introductionmentioning
confidence: 99%
“…In addition, casein micelles have the property of interacting with calcium ions. These ions are responsible for inducing tyrosine phosphorylation to initiate the capacitation process, as well as improving the rate of sperm binding to the zona pellucida of oocytes, with a consequent increase in in vitro embryo production (Da silva et al, 2020;Diniz et al, 2020). Thus, casein has been responsible for the protection of sperm, even at low concentrations in chilled (0.6%) or frozen (1.35%) semen (Lagares et al, 2012).…”
Section: Introductionmentioning
confidence: 99%