Effect of the addition of Peptostreptococcus productus ATCC 35244 on reductive acetogenesis in the ruminal ecosystem after inhibition of methanogenesis by cell-free supernatant of Lactobacillus plantarum 80

Nollet, Leo M.L.; Mbanzamihigo, Léonidas; Demeyer, Daniël; Verstraete, Willy

doi:10.1016/s0377-8401(97)00135-1

Cited by 49 publications

(42 citation statements)

References 29 publications

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“…There is not a clear relationship between the number of methanogens and methanogenesis in the rumen (Nollet et al, 1998;Machmü ller et al, 2003a and2003b;Yanez-Ruiz et al, 2008;Mosoni et al personal communication), except for trials when specific inhibitors of methanogenesis were used (i.e. BSM; Denman et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Microbial ecosystem and methanogenesis in ruminants

Morgavi

Forano²,

Martin

et al. 2010

Animal

539

406

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Ruminant production is under increased public scrutiny in terms of the importance of cattle and other ruminants as major producers of the greenhouse gas methane. Methanogenesis is performed by methanogenic archaea, a specialised group of microbes present in several anaerobic environments including the rumen. In the rumen, methanogens utilise predominantly H 2 and CO 2 as substrates to produce methane, filling an important functional niche in the ecosystem. However, in addition to methanogens, other microbes also have an influence on methane production either because they are involved in hydrogen (H 2 ) metabolism or because they affect the numbers of methanogens or other members of the microbiota. This study explores the relationship between some of these microbes and methanogenesis and highlights some functional groups that could play a role in decreasing methane emissions. Dihydrogen ('H 2 ' from this point on) is the key element that drives methane production in the rumen. Among H 2 producers, protozoa have a prominent position, which is strengthened by their close physical association with methanogens, which favours H 2 transfer from one to the other. A strong positive interaction was found between protozoal numbers and methane emissions, and because this group is possibly not essential for rumen function, protozoa might be a target for methane mitigation. An important function that is associated with production of H 2 is the degradation of fibrous plant material. However, not all members of the rumen fibrolytic community produce H 2 . Increasing the proportion of non-H 2 producing fibrolytic microorganisms might decrease methane production without affecting forage degradability. Alternative pathways that use electron acceptors other than CO 2 to oxidise H 2 also exist in the rumen. Bacteria with this type of metabolism normally occupy a distinct ecological niche and are not dominant members of the microbiota; however, their numbers can increase if the right potential electron acceptor is present in the diet. Nitrate is an alternative electron sinks that can promote the growth of particular bacteria able to compete with methanogens. Because of the toxicity of the intermediate product, nitrite, the use of nitrate has not been fully explored, but in adapted animals, nitrite does not accumulate and nitrate supplementation may be an alternative under some dietary conditions that deserves to be further studied. In conclusion, methanogens in the rumen co-exist with other microbes, which have contrasting activities. A better understanding of these populations and the pathways that compete with methanogenesis may provide novel targets for emissions abatement in ruminant production.

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Section: Discussionmentioning

confidence: 99%

Microbial ecosystem and methanogenesis in ruminants

Morgavi

Forano²,

Martin

et al. 2010

Animal

539

406

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“…A bacteriocin obtained from a rumen bacterium, bovicin HC5, decreased CH 4 production in vitro up to 50% without inducing methanogens' adaptation (Lee et al, 2002). The reported inhibitory effect on methanogenesis of spent culture from Lactobacillus plantarum 80 is also probably induced by a bacteriocin or a similar compound (Nollet et al, 1998). The compound(s) in question reduced numbers of methanogens, but, like many other inhibitors that are efficient in vitro, the effect was lost in sheep after continuous administration for a few days (Nollet et al, 1998).…”

mentioning

confidence: 89%

“…The reported inhibitory effect on methanogenesis of spent culture from Lactobacillus plantarum 80 is also probably induced by a bacteriocin or a similar compound (Nollet et al, 1998). The compound(s) in question reduced numbers of methanogens, but, like many other inhibitors that are efficient in vitro, the effect was lost in sheep after continuous administration for a few days (Nollet et al, 1998). Klieve and Hegarty (1999) also suggested the use of archaeal viruses to decrease the population of methanogens, but, to our knowledge, no bacteriophages active against rumen methanogens have been isolated so far.…”

mentioning

confidence: 96%

“…Attempts to increase the natural rumen population of acetogens have been assayed but without success (Demeyer et al, 1996). The use of acetogens as probiotics has also been tested by several authors with and without the addition of methanogen inhibitors to favour competition (Nollet et al, 1998;Lopez et al, 1999). Results, so far, have been either unsatisfactory or not conclusive.…”

mentioning

confidence: 99%

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Methane mitigation in ruminants: from microbe to the farm scale

Martin

Morgavi

Doreau

2010

Animal

748

657

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Decreasing enteric methane (CH 4 ) emissions from ruminants without altering animal production is desirable both as a strategy to reduce global greenhouse gas (GHG) emissions and as a means of improving feed conversion efficiency. The aim of this paper is to provide an update on a selection of proved and potential strategies to mitigate enteric CH 4 production by ruminants. Various biotechnologies are currently being explored with mixed results. Approaches to control methanogens through vaccination or the use of bacteriocins highlight the difficulty to modulate the rumen microbial ecosystem durably. The use of probiotics, i.e. acetogens and live yeasts, remains a potentially interesting approach, but results have been either unsatisfactory, not conclusive, or have yet to be confirmed in vivo. Elimination of the rumen protozoa to mitigate methanogenesis is promising, but this option should be carefully evaluated in terms of livestock performances. In addition, on-farm defaunation techniques are not available up to now. Several feed additives such as ionophores, organic acids and plant extracts have also been assayed. The potential use of plant extracts to reduce CH 4 is receiving a renewed interest as they are seen as a natural alternative to chemical additives and are well perceived by consumers. The response to tannin-and saponincontaining plant extracts is highly variable and more research is needed to assess the effectiveness and eventual presence of undesirable residues in animal products. Nutritional strategies to mitigate CH 4 emissions from ruminants are, without doubt, the most developed and ready to be applied in the field. Approaches presented in this paper involve interventions on the nature and amount of energy-based concentrates and forages, which constitute the main component of diets as well as the use of lipid supplements. The possible selection of animals based on low CH 4 production and more likely on their high efficiency of digestive processes is also addressed. Whatever the approach proposed, however, before practical solutions are applied in the field, the sustainability of CH 4 suppressing strategies is an important issue that has to be considered. The evaluation of different strategies, in terms of total GHG emissions for a given production system, is discussed.Keywords: methane, greenhouse gases, ruminant, mitigation strategies Implications Methane (CH 4 ) mitigation in ruminants is possible through various strategies. Today, the feeding management approach is the most developed. Other strategies (biotechnologies, additives) are promising but the diversity and plasticity of functions of the rumen bacterial and methanogenic communities may be a limiting factor for their successful application. A possible selection of animals on CH 4 production and more likely on digestive processes is evocated. In any case, before practical solutions are proposed for field application more research in vivo is needed. The sustainability of CH 4 -suppressing strategies is also an important issue and they mi...

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“…In the gut of termites and rodents, acetogens convert excess H 2 to acetic acid, which is then utilized by the host (Joblin 1999). However, in the rumen, acetogens are few and cannot compete effectively with methanogens for H 2 ions, because they have a lower affinity for H 2 than methanogens (Greening and Leedle 1989;Nollet et al 1998).…”

Section: Reductive Acetogenesismentioning

confidence: 99%

Mitigation strategies to reduce enteric methane emissions from dairy cows: Update review

Boadi¹,

Benchaar²,

Chiquette³

et al. 2004

Can. J. Anim. Sci.

416

296

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. 2004. Mitigation strategies to reduce enteric methane emissions from dairy cows: Update review. Can. J. Anim. Sci. 84: 319-335. Enteric methane (CH 4 ) emission is a major contributor to Canadian greenhouse gas emissions, and also a loss of feed energy during production. The objective of this paper is to provide an update on current management practices and new dietary strategies recently proposed to reduce CH 4 emissions from ruminants. Existing mitigation strategies for dairy, e.g., the addition of ionophores, fats, use of high-quality forages, and increased use of grains, have been well researched and applied. These nutritional changes reduce CH 4 emissions by manipulating ruminal fermentation, directly inhibiting methanogens and protozoa, or by diverting hydrogen ions away from methanogens. Current literature has identified new CH 4 mitigation options. These include the addition of probiotics, acetogens, bacteriocins, archaeal viruses, organic acids, plant extracts (e.g., essential oils) to the diet, as well as immunization, and genetic selection of cows. These new strategies are promising, but more research is needed to validate these approaches and to assess in vivo their effectiveness in reducing CH 4 production by dairy cows. It is also important to evaluate CH 4 mitigation strategies in terms of the total greenhouse gas budget and to consider the cost associated with the various strategies. More basic understanding of the natural differences in digestion efficiencies among animals as well as a better knowledge of methanogens and their interaction with other organisms in the rumen would enable us to exploit the potential of some of the new CH 4 mitigation strategies for dairy cattle production. Key words: Enteric methane, dairy cattle, mitigationBoadi, D., Benchaar, C., Chiquette, J. et Massé, D. 2004. Stratégies visant à réduire les dégagements de méthane entérique des vaches laitières: bilan. Can. J. Anim. Sci. 84: 319-335. Le méthane (CH 4 ) d'origine entérique contribue dans une large mesure aux émissions canadiennes de gaz à effet de serre et entraîne une perte de l'énergie tirée des aliments durant la production. Le présent article fait le point sur les pratiques de zootechnie actuelles et sur les stratégies d'engraissement récemment proposées pour réduire les dégagements de CH 4 des ruminants. Les stratégies d'atténuation existantes (addition d'ionophores, matière grasse, utilisation de fourrages de grande qualité, plus grande utilisation de grains) ont fait l'objet de maintes recherches et sont désormais bien appliquées. Les changements nutritionnels qu'elles introduisent diminuent les émissions de CH 4 en modifiant la fermentation dans le rumen, en inhibant directement la production de méthanogènes et la population de protozoaires ainsi qu'en détournant les ions hydrogène des méthanogènes. La documentation scientifique mentionne de nouvelles méthodes pour atténuer la libération de CH 4 . Ces méthodes comprennent l'ajout de probiotiques, d'acétogènes, de bactériocine, de virus archaïques, d'aci...

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Effect of the addition of Peptostreptococcus productus ATCC 35244 on reductive acetogenesis in the ruminal ecosystem after inhibition of methanogenesis by cell-free supernatant of Lactobacillus plantarum 80

Cited by 49 publications

References 29 publications

Microbial ecosystem and methanogenesis in ruminants

Microbial ecosystem and methanogenesis in ruminants

Methane mitigation in ruminants: from microbe to the farm scale

Mitigation strategies to reduce enteric methane emissions from dairy cows: Update review

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