Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film

Chen, Xuefei; Zhou, Lingling; Xu, Huaizhong; Yamamoto, Masaki; Shinoda, Masaya; Kishimoto, Masanori; Tanaka, Tomonari; Yamane, Hideki

doi:10.3390/ma13020377

Cited by 43 publications

(30 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DHT treatment is widely used to improve the properties of collagen-based materials because of its non-cytotoxicity [ 35 , 44 ]. It forms covalent bonds between the collagen peptide chains by condensation reactions under high temperature [ 34 , 45 ]. Compared with the ratio R of ammonia treated collagen tube, ammonia-DHT treated collagen tube had much lower ratio R .…”

Section: Resultsmentioning

confidence: 99%

“…Compared with the ratio R of ammonia treated collagen tube, ammonia-DHT treated collagen tube had much lower ratio R . It showed that ammonia-DHT treatment aggravated the denaturation due to the high treatment temperature [ 34 ]. GTA treatment is a typically chemical crosslinking method which produces the crosslinks through the reaction of the free amine groups of lysine or hydroxylysine amino acids residues of collagen peptide chains with the aldehyde groups of GTA [ 33 , 46 ].…”

Section: Resultsmentioning

confidence: 99%

“…It is easily available, effective, inexpensive and widely used to improve the stability of collagen scaffolds. Dehydrothermal (DHT) treatment, one of common physical treatments, introduces covalent bond between the collagen molecules by condensation reactions under high temperature [ 34 ]. Different crosslinking methods affect the characteristics regarding mechanical properties, degradation, etc., owing to the different mechanism, which should be considered depending on the applications [ 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fabrication and Properties of Electrospun Collagen Tubular Scaffold Crosslinked by Physical and Chemical Treatments

Chen

Meng

et al. 2021

Polymers

Self Cite

View full text Add to dashboard Cite

Tissue engineered scaffold was regarded as a promising approach instead of the autograft. In this study, small diameter electrospun collagen tubular scaffold with random continuous smooth nanofibers was successfully fabricated. However, the dissolution of collagen in concentrated aqueous (conc. aq.) acetic acid caused to the serious denaturation of collagen. A novel method ammonia treatment here was adopted which recovered the collagen triple helix structure according to the analysis of IR spectra. Further dehydrothermal (DHT) and glutaraldehyde (GTA) treatments were applied to introduce the crosslinks to improve the properties of collagen tube. The nanofibrous structure of collagen tube in a wet state was preserved by the crosslinking treatments. Swelling ratio and weight loss decreased by at least two times compared to those of the untreated collagen tube. Moreover, tensile strength was significantly enhanced by DHT treatment (about 0.0076 cN/dTex) and by GTA treatment (about 0.075 cN/dTex). In addition, the surface of crosslinked collagen tube kept the hydrophilic property. These results suggest that DHT and GTA treatments can be utilized to improve the properties of electrospun collagen tube which could become a suitable candidate for tissue engineered scaffold.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication and Properties of Electrospun Collagen Tubular Scaffold Crosslinked by Physical and Chemical Treatments

Chen

Meng

et al. 2021

Polymers

Self Cite

View full text Add to dashboard Cite

show abstract

“…This is not easy to understand but the explanation probably lies in the relative amounts of functional groups and the proximity between them. Xuefei et al [ 37 ] demonstrated that DHT cross-linking of collagen resulted in formation of both covalent and hydrogen bonding. The bonding between the peptide chains seems to be prevalent, as it is proven by the reduction in the number of free acidic and basic residues on collagen molecules after the DHT treatment.…”

Section: Resultsmentioning

confidence: 99%

Blending Gelatin and Cellulose Nanofibrils: Biocomposites with Tunable Degradability and Mechanical Behavior

et al. 2020

View full text Add to dashboard Cite

Many studies show how biomaterial properties like stiffness, mechanical stimulation and surface topography can influence cellular functions and direct stem cell differentiation. In this work, two different natural materials, gelatin (Gel) and cellulose nanofibrils (CNFs), were combined to design suitable 3D porous biocomposites for soft-tissue engineering. Gel was selected for its well-assessed high biomimicry that it shares with collagen, from which it derives, while the CNFs were chosen as structural reinforcement because of their exceptional mechanical properties and biocompatibility. Three different compositions of Gel and CNFs, i.e., with weight ratios of 75:25, 50:50 and 25:75, were studied. The biocomposites were morphologically characterized and their total- and macro- porosity assessed, proving their suitability for cell colonization. In general, the pores were larger and more isotropic in the biocomposites compared to the pure materials. The influence of freeze-casting and dehydrothermal treatment (DHT) on mechanical properties, the absorption ability and the shape retention were evaluated. Higher content of CNFs gave higher swelling, and this was attributed to the pore structure. Cross-linking between CNFs and Gel using DHT was confirmed. The Young’s modulus increased significantly by adding the CNFs to Gel with a linear relationship with respect to the CNF amounts. Finally, the biocomposites were characterized in vitro by testing cell colonization and growth through a quantitative cell viability analysis performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Additionally, the cell viability analysis was performed by the means of a Live/Dead test with Human mesenchymal stem cells (hMSCs). All the biocomposites had higher cytocompatibility compared to the pure materials, Gel and CNFs.

show abstract

“…After lyophilization, The SF-ELP (S-ELP) complex was obtained and stored at 4 °C for further use. Next, according to previously reported protocol [ 31 ], we performed DHT treatment of the S-ELP complexes. When the S-ELP complexes are heated up to a critical temperature, crosslinked bonds between the chains of amino acids present in the SF and ELP molecules would form by esterification of carboxyl and hydroxyl groups and amidation between carboxyl and amino groups.…”

Section: Methodsmentioning

confidence: 99%

Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair

Chen

Zhang

et al. 2021

Bioactive Materials

View full text Add to dashboard Cite

Silk fibroin (SF) is considered biocompatible and biodegradable for osteochondral repair. However, it lacks a bioactive domain for cell adhesion, proliferation and differentiation, limiting its therapeutic efficacy. To revamp SF as a biomimicking and bioactive microenvironment to regulate cell behaviours, we engineered an elastin-like polypeptide (ELP, Val-Pro-Gly-Xaa-Gly) to modify SF fibers via simple and green dehydrothermal (DHT) treatment. Our results demonstrated that the ELP successfully bound to SF, and the scaffold was reinforced by the fusion of the silk fiber intersections with ELP (S-ELP-DHT) via the DHT treatment. Both bone mesenchymal stem cells (BMSCs) and chondrocytes exhibited improved spreading and proliferation on the S-ELP-DHT scaffolds. The ex vivo and in vivo experiments further demonstrated enhanced mature bone and cartilage tissue formation using the S-ELP-DHT scaffolds compared to the naked SF scaffolds. These results indicated that a recombinant ELP-modified silk scaffold can mimic three-dimensional (3D) cell microenvironment, and improve bone and cartilage regeneration. We envision that our scaffolds have huge clinical potential for osteochondral repair.

show abstract

Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film

Cited by 43 publications

References 29 publications

Fabrication and Properties of Electrospun Collagen Tubular Scaffold Crosslinked by Physical and Chemical Treatments

Fabrication and Properties of Electrospun Collagen Tubular Scaffold Crosslinked by Physical and Chemical Treatments

Blending Gelatin and Cellulose Nanofibrils: Biocomposites with Tunable Degradability and Mechanical Behavior

Elastin-like polypeptide modified silk fibroin porous scaffold promotes osteochondral repair

Contact Info

Product

Resources

About