Effect of the coffee ingredient cafestol on head and neck squamous cell carcinoma cell lines

Kotowski, Ulana; Heiduschka, Gregor; Seemann, Rudolf; Eckl‐Dorna, Julia; Schmid, Rainer; Kranebitter, Veronika; Stanisz, Isabella; Brunner, Markus; Lill, Claudia; Thurnher, Dietmar

doi:10.1007/s00066-014-0807-x

Cited by 16 publications

(8 citation statements)

References 21 publications

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“…. Tumor cells were cultured as previously described (10). In brief, the cells were grown in RPMI medium (Cambrex, Walkersville, MD, USA) supplemented with 10% fetal bovine serum (PAA Laboratories, Linz, Austria) and 1% penicillin-streptomycin (Gibco BRL, Gaithersburg, MD, USA) at 37˚C in a humidified atmosphere of 5% CO 2 .…”

Section: Introductionmentioning

confidence: 99%

Effect of thymoquinone on head and neck squamous cell carcinoma cells in vitro: Synergism with radiation

et al. 2017

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Abstract. Thymoquinone (TQ) is the main bioactive constituent present in black seed oil (Nigella sativa); it has shown anti-inflammatory and anti-neoplastic effects in various cancer cell types. The aim of the present study was to investigate the effects of TQ on head and neck squamous cell carcinoma (HNSCC) cell lines, on its own and in combination with radiation and cisplatin, respectively. The SCC25 and CAL27 HNSCC cell lines were treated with TQ alone and in combination with cisplatin or radiation, respectively. Proliferation assays and clonogenic assays were performed. Apoptosis was detected by flow cytometry. TQ exhibited dose-dependent cytotoxicity via apoptosis in the investigated cell lines. In combination with cisplatin, TQ resulted in no significant increase in cytotoxicity. Combined with radiation, TQ significantly reduced clonogenic survival compared with each treatment method alone. TQ is a promising agent in the treatment of head and neck cancer due to its anti-proliferative and radiosensitizing properties. However, the combination of TQ with cisplatin showed no therapeutic benefit in vitro.

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Section: Introductionmentioning

confidence: 99%

Effect of thymoquinone on head and neck squamous cell carcinoma cells in vitro: Synergism with radiation

et al. 2017

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“…Thermoluminescence dosimetry was performed to measure the radiation dose for the following experiments. After 72 hours, growth inhibition was determined by CCK‐8 assay and combination index values were calculated, as described previously …”

Section: Methodsmentioning

confidence: 99%

“…To assess long‐term effect of resminostat on irradiated cells, clonogenic assays were conducted in accord with the protocol of Franken et al In brief, 3 × 10 2 to 12 × 10 2 cells were seeded into 6‐well plates. After 24 hours, cells were treated with 1.25 μM or 2.5 μM resminostat and irradiated with 2, 4, 6, or 8 Gy using a conventional radiation source with a 150 kV X‐ray machine, as previously described . After 72 hours, drug‐containing medium was exchanged with drug‐free RPMI medium.…”

Section: Methodsmentioning

confidence: 99%

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Effect of the histone deacetylase inhibitor resminostat on head and neck squamous cell carcinoma cell lines

et al. 2017

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“…Similarly, cafestol has also been noted to cause apoptosis by cleavages of the Bid and caspase‐3 in mesothelioma cells . It has been also suggested to reduce apoptotic cell death and clonogenic survival in SCC25, CAL27, and FaDu cell lines . On the other hand, ferulic acid has been reported to results into an apoptotic cell death at 500 μM of concentration after 72 h of exposure in cultured cell lines and MIA PaCa‐2 cells .…”

Section: Effects On Apoptosismentioning

confidence: 99%

An insight towards anticancer potential of major coffee constituents

et al. 2018

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Cancer is a complex disease that transforms a normal cell into a malignant cell by disturbing different molecular mechanisms. Lately, plant‐derived bioactive products have gained prominent attention to serve as anti‐cancer agents. These natural anti‐neoplastic agents are believed to act as alternatives for the synthetic drugs or could be used to enhance the prospect of other drugs in reducing their dose, thus limiting their possible toxic effects. They could also counterbalance the other anti‐cancer drug‐induced adverse effects. Among natural plant‐derived products, coffee has been reported for its significant anti‐carcinogenic effects in the scientific literature. This article aims to highlight the anti‐cancer potential of different coffee components viz. caffeic acid, chlorogenic acids, caffeine (1,3,7‐trimethylxanthine), cafestol, ferulic acid, and kahweol together in a single article. Based on our article, it is quite clear that these bioactive components have important therapeutic potentials against cancerous cells. However, the lack of clinical data negates their use in humans. Therefore, more research is recommended to achieve the desired pharmaceutical value. We also implore assessing their safety and possible adverse effects prior to their progress into clinical trials. © 2018 BioFactors, 44(4):315–326, 2018

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Effect of the coffee ingredient cafestol on head and neck squamous cell carcinoma cell lines

Abstract: Cafestol is a naturally occurring effective compound with growth-inhibiting properties in head and neck cancer cells. Moreover, it leads to a significant inhibition of colony formation.

Cited by 16 publications

References 21 publications

Effect of thymoquinone on head and neck squamous cell carcinoma cells in vitro: Synergism with radiation

Effect of thymoquinone on head and neck squamous cell carcinoma cells in vitro: Synergism with radiation

Effect of the histone deacetylase inhibitor resminostat on head and neck squamous cell carcinoma cell lines

An insight towards anticancer potential of major coffee constituents

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