Effect of the combined use of enamel matrix derivative and atelocollagen sponge scaffold on osteoblastic differentiation of mouse induced pluripotent stem cells in vitro

Abstract

These data suggest that combining ACS with EMD increases levels of osteoblastic differentiation and mineralization in miPS-EB-derived cells, compared with ACS used alone.

Effects of intermittent treatment with parathyroid hormone (PTH) on osteoblastic differentiation and mineralization of mouse induced pluripotent stem cells in a 3D culture model

“…It is important to note that PTH treatment had no significant effect on the expression of Bglap, one of the key osteoblastic markers. A similar result was obtained in a previous study by our research group, 13 in which we investigated the effects of EMD on the miPS-EB-derived into intracellular compartments 38 and recycled to the membrane, causing receptor re-sensitization. 39 Once these complexes are targeted for degradation, receptor down regulation is observed.…”

“…Zujur et al 33 reported that, in comparison with 2D conditions, the 3D-cultured miPSC-derived mesodermal cells exhibited accelerated osteogenic differentiation, giving rise to functional osteoblast-osteocyte populations within calcified structures. Moreover, in our previous study, 13 we showed that combining EMD with ACS enhanced osteoblastic differentiation and mineralization in miPS-EB-derived cells. Taking these into consideration, the accelerated outcomes we observed might be due to the synergetic effects of the intermittent PTH treatment and the scaffold-based 3D culture system.…”

“…Our group has been conducting research on the development of efficient method for osteogenic differentiation of bone marrow MSCs, periodontal ligament (PDL)-derived cells, and iPSCs. 12,13 Previously, we showed that combining EMD with an atelocollagen sponge (ACS) enhances osteoblastic differentiation and mineralization of iPSCs. 13 To gain more information and develop optimum protocols for osteoblastic differentiation of iPSCs, we have been searching for the potent agent(s) for inducing osteogenic cells from iPSCs.…”

“…12,13 Previously, we showed that combining EMD with an atelocollagen sponge (ACS) enhances osteoblastic differentiation and mineralization of iPSCs. 13 To gain more information and develop optimum protocols for osteoblastic differentiation of iPSCs, we have been searching for the potent agent(s) for inducing osteogenic cells from iPSCs. To this date, only anabolic reagent for osteogenic induction used clinically is parathyroid hormone (PTH) which is secreted from parathyroid gland.…”

Utility of High-Intensity Laser Therapy Combined with Photobiomodulation Therapy for Socket Preservation After Tooth Extraction

“…These phenomena were similar to the finding that an artificial collagen sponge implanted in the extraction socket promotes the differentiation and proliferation of mesenchymal stem cells and provides a scaffold for bone formation. 40,41 Recently, tissue activation by PBMT was shown to be strongly associated with mitochondrial adenosine tri-phosphate (ATP) production facilitated by cytochrome C oxidase, but the precise effects of the laser wavelengths used in the present study remain to be clarified.…”