Effect of the Contents and Form of Rabies Glycoprotein on the Potency of Rabies Vaccination in Cattle

Piza, A. T.; Pieri, K. M. S.; Lusa, G. M.; Caporale, Graciane Maria Medeiros; Terreran, Maria Thereza; Machado, L. A.; Zanetti, Carlos Roberto

doi:10.1590/s0074-02762002000200022

Cited by 22 publications

(19 citation statements)

References 17 publications

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“…The importance of a booster dose was also reported by several researchers (1,2,17,20,21,23), confirming that the immune response induced by only one vaccine dose does not induce high antibody titers. However, when boosters are given, the serum neutralizing antibody titers become significantly higher (1,16).…”

supporting

confidence: 67%

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Importance of Antirabies Revaccination for an Adequate Antirabies Protection in Bovine Newborns

Filho

Megid

Geronutti

et al. 2010

Clin Vaccine Immunol

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The transfer of antirabies immunoglobulins in cows that were prime vaccinated and cows that were revaccinated against rabies correlated to the serum titers in their offspring was evaluated. The results demonstrated that revaccination against rabies during pregnancy induces neutralizing antibody titers at a protective level that are transferred directly to calves through colostrum and reinforce the importance of revaccination for improved colostral antibody transfer and offspring protection against rabies.According to the Panamerican Health Organization (16), 2,797 cases of rabies in cattle were reported in 2004 in the Americas, which is an 87% increase over 2002 levels. Of these, 2,591 (92.6%) occurred in Latin America and 130 (4.6%) in North America. In Latin America, Brazil had 60.6% of the cases, followed by 13% in Mexico and 8% in the Andes. More recent data demonstrated that Brazil still reports more cases of rabies in domestic animals than other countries of South America, with a predominance of rabies in cattle (24).

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supporting

confidence: 67%

“…Other researchers have also reported similar results (17,20,21,23), demonstrating that the immune response induced by only one vaccine did not induce high antibody titers; however, when boosters are given, titers become significantly higher (1,2,15).…”

supporting

confidence: 55%

Importance of Antirabies Revaccination for an Adequate Antirabies Protection in Bovine Newborns

Filho

Megid

Geronutti

et al. 2010

Clin Vaccine Immunol

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“…Many successful vaccines depend on particle formation to enhance vaccine potency. These include inactivated viral particles, such as inactivated polio vaccine (48) and rabies (35), and recombinant proteins that form virus-like particles, such as HBsAg (9,40) and human papillomavirus (HPV) (18). A number of experimental vaccines have gained potency by linking antigens with HBsAg to form virus-like particles, including vaccines against malaria (43) and hepatitis C virus (30).…”

Section: Discussionmentioning

confidence: 99%

Hepatitis B Virus Surface Antigen Assembly Function Persists when Entire Transmembrane Domains 1 and 3 Are Replaced by a Heterologous Transmembrane Sequence

Berkower

Spadaccini

Chen

et al. 2011

J Virol

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Native hepatitis B surface antigen (HBsAg) spontaneously assembles into 22-nm subviral particles. The particles are lipoprotein micelles, in which HBsAg is believed to span the lipid layer four times. The first two transmembrane domains, TM1 and TM2, are required for particle assembly. We have probed the requirements for particle assembly by replacing the entire first or third TM domain of HBsAg with the transmembrane domain of HIV gp41. We found that either TM domain of HBsAg could be replaced, resulting in HBsAg-gp41 chimeras that formed particles efficiently. HBsAg formed particles even when both TM1 and TM3 were replaced with the gp41 domain. The results indicate remarkable flexibility in HBsAg particle formation and provide a novel way to express heterologous membrane proteins that are anchored to a lipid surface by their own membrane-spanning domain. The membrane-proximal exposed region (MPER) of gp41 is an important target of broadly reactive neutralizing antibodies against HIV-1, and HBsAg-MPER particles may provide a good platform for future vaccine development.HBsAg is the surface antigen of hepatitis B virus. In the virus, translation of the same open reading frame can begin at one of three in-frame start codons, resulting in proteins of large (42 kDa), medium (33 kDa), and small size (27 kDa), which share the identical 226-amino-acid sequence at the carboxyl end. The 27-kDa form of HBsAg is fully capable of spontaneous assembly into virus-like particles, so we have focused on this protein to study the requirements for particle assembly. In nature, small HBsAg participates in forming at least four different kinds of particles: 42-nm infectious hepatitis B virions (Dane particles), 22-nm spherical subviral particles, 22-nm-diameter rods, or as the borrowed envelope protein in 36-nm hepatitis D virus (39, 45).Recombinant-derived small HBsAg forms subviral particles when expressed in a number of different cell types, including mammalian (39), yeast (25), and insect cells (19), but not Escherichia coli (25,26). The particles are lipoprotein micelles, which contain 25% lipid. In these particles, HBsAg is believed to span the lipid four times, with four transmembrane domains (TM1 to TM4). This conformation exposes three external domains on the lipid surface at the amino end, middle, and carboxyl end of the protein (7, 42). Prior studies, based on the deletion of membrane-spanning domains, showed that transmembrane domains TM1 and TM2 were required for particle formation (5, 6). However, subsequent studies have shown that, at least for TM1, the replacement of amino acids is permitted (21,33). In this paper, we have substituted an entire TM domain of another transmembrane protein (HIV gp41) for TM1 or TM3 of HBsAg and probed the requirements for particle assembly.When other proteins were expressed in tandem with HBsAg, the surface antigen acted as a carrier protein and incorporated proteins as large as gp120 into the particles (2). This method allowed us to express membrane proteins and transmembrane proteins...

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“…Isso representa prejuízos diretos de 15 milhões de dólares e indiretos de 22,5 milhões de dólares. Para fazer o controle desta enfermidade, a vacinação contínua dos bovinos é o método mais efetivo, de menor custo e reduz as perdas econômicas LIMA et al, 2005;PIZA et al, 2002).…”

Section: Introductionunclassified

Avaliação da vacinação anti-rábica e da suplementação com probiótico na resposta imune humoral em bovinos

et al. 2009

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ResumoObjetivou-se avaliar a resposta imune humoral a uma nova vacina anti-rábica, desenvolvida no Instituto Butantan em bovinos primovacinados e o efeito do probiótico nesta resposta. Trinta e quatro bovinos da raça Nelore com idade de 15 meses foram divididos aleatoriamente em 2 grupos (17 bovinos/grupo): os animais foram vacinados no dia zero e um dos grupos recebeu uma mistura mineral com probiótico (GP), enquanto o outro apenas a mistura (GC). Colheu-se sangue dos animais nos dias 0, 75 e 150 após a vacinação para determinação dos títulos de anticorpos anti-rábicos neutralizantes pela técnica de soroneutralização em células BHK 21 (RFFIT). Foram encontrados títulos de anticorpos protetores (≥0,5 UI/mL) em 82,4% dos animais do grupo GP e 76,5% do grupo GC. Não houve diferença significativa (p>0,05) nos títulos de anticorpos entre os soros coletados dos dois grupos de animais nos dias 75 e 150. Verificou-se também que para ambos os grupos no dia 150 houve uma redução significativa (p<0,01) nos títulos de anticorpos. Conclui-se que a vacina anti-rábica é eficiente em produzir soroconversão e em manter os títulos de anticorpos em bovinos primovacinados. A ingestão do probiótico não interferiu na resposta imune humoral anti-rábica. Palavras-chave: Probiótico, resposta imune humoral, vacina anti-rábica, anticorpos, bovino AbstractThis study evaluated the humoral immune response of a new rabies vaccine developed by the Instituto Butantan (potency of 3.27 UI/ml) in primovaccinated cattle and the effect of probiotic on this response. Thirty-four 15-month old Nelore cattle were randomly divided into 2 groups (17 animals/group). All the animals were vaccinated on day 0 (zero) and then animals in one group received probiotic added to a mineral mixture (GP) while the others were given only the mineral mixture (GC). Blood samples were collected on days 0, 75 and 150 for rabies neutralizing antibodies titers by seroneutralization assay on BHK 21 cells (RFFIT). Protective antibody titers (≥0.5 UI/mL) were found in 82.4% of the animals from GP and in 76.5% of the animals from GC and no statistical difference (p>0.05) between antibody titers in GP and GC was detected on days 75 and 150. It was also observed that in both groups antibody titers was decreased on day 150 (p<0.01). In conclusion, the tested rabies vaccine promotes efficient soroconversion and keeps antibody levels in primovaccinated cattle, but probiotic does not affect the humoral anti-rabies immune response.

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Effect of the Contents and Form of Rabies Glycoprotein on the Potency of Rabies Vaccination in Cattle

Cited by 22 publications

References 17 publications

Importance of Antirabies Revaccination for an Adequate Antirabies Protection in Bovine Newborns

Importance of Antirabies Revaccination for an Adequate Antirabies Protection in Bovine Newborns

Hepatitis B Virus Surface Antigen Assembly Function Persists when Entire Transmembrane Domains 1 and 3 Are Replaced by a Heterologous Transmembrane Sequence

Avaliação da vacinação anti-rábica e da suplementação com probiótico na resposta imune humoral em bovinos

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