Electrospinning is known as a versatile technique for articular cartilage tissue regeneration (ACTR) due to its excellent potential to produce a brous scaffold that mimics the extracellular matrix (ECM) of native tissue. However, there is a need to promote the biological performance of scaffolds maintaining their mechanical strength. In this study, a core-shell polyhydroxybutyrate (PHB)-starch/halloysite nanotubes (HNTs) @ ECM-chitosan (Cs) scaffold was prepared via the coaxial electrospinning method. The results exhibited a narrower ber diameter of up to 164 ± 24 nm with an appropriate pore size and porosity after incorporating Cs and ECM. Moreover, the core-shell scaffold showed an enhanced Young's modulus up to 4.45 ± 0.1 MPa that could support chondrocyte cell growth. After that, the wettability and in vitro degradability of the core-shell scaffold were induced due to the hydrophilic nature of shell components. Also, chondrocyte cells had more viability and attachment on the core-shell structure proving the potential of core-shell bers for biomedical applications. In conclusion, the results showed that the core-shell