Effect of the flavonol quercetin on ion transport in the isolated intestine of the eel, Anguilla anguilla

Trischitta, F.; Faggio, Caterina

doi:10.1016/j.cbpc.2005.11.012

Cited by 13 publications

(6 citation statements)

References 24 publications

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“…RTgutGC cells translate the expression of epithelial barrier genes into the formation of a functional barrier, as indicated by increasing TEER values measured at regular intervals up to 21 days after seeding [ 18 , 23 , 30 , 31 , 32 ]. Both RTpi-MI and RTdi-MI cell lines developed similar TEER values between 3 and 21 days of culture, which was close to the baseline resistance (50–400 Ω cm 2 ) measured in in vivo studies [ 33 , 34 ]. These values are consistent with those of fish intestine and RTgutGC cells [ 23 ] that are considered as “leaky” epithelia [ 35 ].…”

Section: Discussionsupporting

confidence: 79%

New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo

Pasquariello

Verdile

Pavlović

et al. 2021

Cells

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We derived two novel cell lines from rainbow trout (RT) proximal (RTpi-MI) and distal intestine (RTdi-MI) and compared them with the previously established continuous cell line RTgutGC. Intestinal stem cells, differentiating and differentiated epithelial cells, and connective cells were found in all cell lines. The cell lines formed a polarized barrier, which was not permeable to large molecules and absorbed proline and glucose. High seeding density induced their differentiation into more mature phenotypes, as indicated by the downregulation of intestinal stem cell-related genes (i.e., sox9, hopx and lgr5), whereas alkaline phosphatase activity was upregulated. Other enterocyte markers (i.e., sglt1 and pept1), however, were not regulated as expected. In all cell lines, the presence of a mixed population of epithelial and stromal cells was characterized for the first time. The expression by the stromal component of lgr5, a stem cell niche regulatory molecule, may explain why these lines proliferate stably in vitro. Although most parameters were conserved among the three cell lines, some significant differences were observed, suggesting that characteristics typical of each tract are partly conserved in vitro as well.

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Section: Discussionsupporting

confidence: 79%

New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo

Pasquariello

Verdile

Pavlović

et al. 2021

Cells

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“…An increasing body of literature demonstrates that functional feed ingredients can support intestinal health and reduce disease susceptibility via multiple mechanisms, including direct effects on a variety of intestinal functions, e.g., barrier function, nutrient transport and immune responses (7, 23, 64–66). In fish, knowledge about basic mechanisms of functional ingredients and their interactions with the intestinal tissue is weak and fragmentary.…”

Section: Resultsmentioning

confidence: 99%

Rainbow Trout (Oncorhynchus Mykiss) Intestinal Epithelial Cells as a Model for Studying Gut Immune Function and Effects of Functional Feed Ingredients

et al. 2019

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The objective of this study was to evaluate the suitability of the rainbow trout intestinal epithelial cell line (RTgutGC) as an in vitro model for studies of gut immune function and effects of functional feed ingredients. Effects of lipopolysaccharide (LPS) and three functional feed ingredients [nucleotides, mannanoligosaccharides (MOS), and beta-glucans] were evaluated in RTgutGC cells grown on conventional culture plates and transwell membranes. Permeation of fluorescently-labeled albumin, transepithelial electrical resistance (TEER), and tight junction protein expression confirmed the barrier function of the cells. Brush border membrane enzyme activities [leucine aminopeptidase (LAP) and maltase] were detected in the RTgutGC cells but activity levels were not modulated by any of the exposures. Immune related genes were expressed at comparable relative basal levels as these in rainbow trout distal intestine. LPS produced markedly elevated gene expression levels of the pro-inflammatory cytokines il1b, il6, il8, and tnfa but had no effect on ROS production. Immunostaining demonstrated increased F-actin contents after LPS exposure. Among the functional feed ingredients, MOS seemed to be the most potent modulator of RTgutGC immune and barrier function. MOS significantly increased albumin permeation and il1b, il6, il8, tnfa, and tgfb expression, but suppressed ROS production, cell proliferation and myd88 expression. Induced levels of il1b and il8 were also observed after treatment with nucleotides and beta-glucans. For barrier function related genes, all treatments up-regulated the expression of cldn3 and suppressed cdh1 levels. Beta-glucans increased TEER levels and F-actin content. Collectively, the present study has provided new information on how functional ingredients commonly applied in aquafeeds can affect intestinal epithelial function in fish. Our findings suggest that RTgutGC cells possess characteristic features of functional intestinal epithelial cells indicating a potential for use as an efficient in vitro model to evaluate effects of bioactive feed ingredients on gut immune and barrier functions and their underlying cellular mechanisms.

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“…Previous studies have investigated antioxidant systems via xenobiotic exposure after natural antioxidant materials have been supplied (Pinho et al, 2005;Weng et al, 2007;Prieto et al, 2008;Chen et al, 2009). Few studies about quercetin in fish have been performed (Weber et al, 2002;Trischitta and Faggio, 2006;Park et al, 2008), but studies on antioxidant systems by environmental stress (e.g., changes in salinity) in fish have not been performed.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Quercetin on Physiological Characteristics and Oxidative Stress Resistance in Olive Flounder, Paralichthys olivaceus

Shin¹,

Yoo²,

Min³

et al. 2010

Asian Australas. J. Anim. Sci

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We investigated the effect of quercetin on growth and plasma cholesterol level and the effects of quercetin pretreatment (Diet 1, 0%; Diet 2, 0.25%; and Diet 3, 0.5% quercetin) for 30 and 60 days on oxidative stress induced by hypo-osmotic conditions (17.5, 8.75, and 4 psu) in olive flounder. The weights of flounder were higher with Diet 3 than with Diet 1 and 2, which indicated that a high concentration (Diet 3) of quercetin was very effective in growth. Total cholesterol levels were lower with Diets 2 and 3 than with Diet 1, leading us to hypothesize that quercetin removed low-density lipoproteins from circulation and thereby reduced total cholesterol. To understand the antioxidant role of quercetin, we measured the mRNA expression and activities of superoxide dismutase (SOD) and catalase (CAT) and the H 2 O 2 concentration in quercetin-treated flounder exposed to osmotic stress. The H 2 O 2 concentration and the SOD and CAT expression and activity levels were lower in flounder fed with Diets 2 and 3 than with Diet 1, suggesting that quercetin directly scavenges reactive oxygen species to reduce oxidative stress. Furthermore, the plasma lysozyme activity and osmolality were higher with Diets 2 and 3 than with Diet 1, indicating that quercetin increases immune function and helps to maintain physiological homeostasis. Plasma cortisol was lower with Diets 2 and 3 than with Diet 1, suggesting the quercetin protects against stress. These results indicate that quercetin has hypocholesterolemic and antioxidant effects, increases immune function, and acts to maintain physiological homeostasis.

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Effect of the flavonol quercetin on ion transport in the isolated intestine of the eel, Anguilla anguilla

Cited by 13 publications

References 24 publications

New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo

New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo

Rainbow Trout (Oncorhynchus Mykiss) Intestinal Epithelial Cells as a Model for Studying Gut Immune Function and Effects of Functional Feed Ingredients

The Effects of Quercetin on Physiological Characteristics and Oxidative Stress Resistance in Olive Flounder, Paralichthys olivaceus

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