Effect of the genotype for malignant hyperthermia as determined by a restriction endonuclease assay on the quality characteristics of commercial pork loins1

Pommier, Serge A.; Houde, Alain

doi:10.2527/1993.712420x

Cited by 56 publications

(33 citation statements)

References 15 publications

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“…Contrary to us, some other studies found no differences between N/N and N/n pigs for colour (De Smet et al, 1996;Larzul et al, 1997;Fernandez et al, 2002;de Oliveira Band et al, 2005). Regarding the water holding capacity of LD muscle, significantly higher drip loss obtained in our study for N/n than N/N pigs agrees well with numerous results for various measurement methods (Sather et al, 1991;Pommier and Houde, 1993;Casteels et al, 1995;De Smet et al, 1996;Leach et al, 1996;Fisher et al, 2000;Hamilton et al, 2000;de Oliveira Band et al, 2005). …”

Section: Effect Of Ryr1 Genotypesupporting

confidence: 80%

“…The presence of "n" allele influences the rate of pH fall (Gueblez et al, 1995;De Smet et al, 1996;Larzul et al, 1997;Monin et al, 1999;Fisher et al, 2000;Krenková et al, 2001) but is not related to the ultimate pH, in accordance with similar glycolytic potential observed for N/n and N/N pigs (Larzul et al, 1997). Nevertheless, some authors (Pommier and Houde, 1993;Leach et al, 1996;Hamilton et al, 2000) reported lower pH u of N/n compared to N/N pigs. Significantly lighter colour of N/n compared to N/N pigs in the present study corroborates the results of Pommier and Houde (1993) and Fisher et al (2000).…”

Section: Effect Of Ryr1 Genotypementioning

confidence: 64%

“…Nevertheless, some authors (Pommier and Houde, 1993;Leach et al, 1996;Hamilton et al, 2000) reported lower pH u of N/n compared to N/N pigs. Significantly lighter colour of N/n compared to N/N pigs in the present study corroborates the results of Pommier and Houde (1993) and Fisher et al (2000). This result could be explained by a higher rate of pH fall in N/n pigs, however it is difficult to confirm that since we did not measure it.…”

Section: Effect Of Ryr1 Genotypementioning

confidence: 99%

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Comparison of PRKAG3 and RYR1 gene effect on carcass traits and meat quality in Slovenian commercial pigs

Škrlep¹,

Kavar²,

Čandek-Potokar³

2010

Czech J. Anim. Sci.

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ABSTRACT:The effect of polymorphisms at PRKAG3 (R200Q and I199V) and RYR1 (R615C) genes on carcass traits and meat quality was examined in a sample of 257 commercial pigs, crosses of Landrace × Large White as maternal line and Pietrain (N = 96), Pietrain × Landrace (N = 42) or Pietrain × Hampshire (N = 119) as paternal line. Pigs were genotyped (PCR-RFLP) and traits of interest were measured (which included carcass and ham weight, measurements of fatness, meatiness, ultimate pH, colour parameters and drip loss). The observed genotype frequencies at PRKAG3 gene were 9.7%, 38.9%, 32.7%, 6.2% and 12. 5% for R/R-I/I, R/R-I/V, R/R-V/V, Q/R-I/Vand Q/R-V/V genotype, respectively. RYR1 genotype frequencies were 57.2% for N/N and 42.8% for N/n genotype. Studied polymorphisms exhibited a significant effect on meat quality, but mainly an insignificant effect on carcass traits. No significant interaction between PRKAG3 and RYR1 was found. Carriers of RYR1 mutant allele "n" had less intense longissimus dorsi muscle colour (subjective score, Minolta L* and b*) and higher drip loss. Regarding PRKAG3, the ultimate pH decreased and Minolta L*, a*, b* and drip loss increased in the following order: R/R-I/I, R/R-I/V, R/R-V/V, Q/R-I/V and Q/R-V/V, according to the presence of 199I and absence of 200Q alleles. The study shows that the I199V polymorphism is an important source of variation in pigs free of 200Q. In particular the 199I proves beneficial for meat quality. The results of combining the RYR1 and PRKAG3 genotypes indicate that R/R-I/I genotype could be used in counterbalancing the negative effects of "n" allele on meat quality.

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Section: Effect Of Ryr1 Genotypesupporting

confidence: 80%

Section: Effect Of Ryr1 Genotypementioning

confidence: 64%

Section: Effect Of Ryr1 Genotypementioning

confidence: 99%

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Comparison of PRKAG3 and RYR1 gene effect on carcass traits and meat quality in Slovenian commercial pigs

Škrlep¹,

Kavar²,

Čandek-Potokar³

2010

Czech J. Anim. Sci.

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“…As a consequence, in recessive homozygotes (nn) the gene hal leads to PSS and the major post-mortem manifestation of pale, soft and exudative pork (PSE). In heterozygosis (Nn) the hal gene produces lower carcass quality (Prommier and Houde, 1993;Cheak et al, 1994;Webb, 1996), but possibly higher carcass weight (Zhang et al, 1992;Leach et al, 1996;Fávero, 1997). The polymorphism at nucleotide 1843 of the hal gene has recently been characterized by a DNA test using blood or a muscle biopsy as the source of genomic DNA Cheak et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Characterization of swine stress gene by DNA testing using plucked hair as a source of DNA

Bastos¹,

Federizzi²,

Deschamps³

et al. 2000

Genet. Mol. Biol.

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The swine stress gene (hal) in recessive homozygotes (nn) leads to porcine stress syndrome (PSS), and is associated with pale, soft, exudative pork (PSE). In heterozygosis (Nn) it is linked to poor carcass quality. A total of 179 pigs (86 Large White, 69 Landrace, 12 Duroc and 12 Pietrain) were characterized as normal homozygotes (NN), heterozygotes or recessive homozygotes following amplification of a target region of the hal gene using the polymerase chain reaction (PCR), followed by a restriction endonuclease assay. Plucked hair was used as a source of genomic DNA. The resulting PCR was digested with the restriction enzyme CfoI, followed by agarose gel electrophoresis. Of 179 animals tested, 70% were NN, 28% were Nn, and 2% were nn. The frequency of heterozygotes was higher (P < 0.05) in Landrace (0.43 for Nn) than in Large White pigs (0.09 for Nn). Nine of the 12 Pietrain animals were Nn and three were nn, suggesting a high frequency for the n allele in this breed. These results may be related to the incidence of PSS and PSE in these two breeds, both of which are widely used in breeding programs. The utilization of plucked hair as the source of genomic DNA was a non-invasive and quick method to screen farm animals.
O gene do estresse suíno (hal) em homozigose recessiva (nn) ocasiona a síndrome do estresse porcino (PSS), e está relacionado com a ocorrência da carne pálida, mole e exudativa (PSE). Em heterozigose (Nn) está associado com diminuição da qualidade de carcaça. Um total de 179 suínos (86 Large White, 69 Landrace, 12 Duroc e 12 Pietrain) foram caracterizados como homozigotos normais (NN), heterozigotos e homozigotos recessivos por análise do DNA usando a reação de polimerização em cadeia (PCR), seguida de um ensaio com endonuclease de restrição. Foi utilizado folículo piloso como fonte de DNA genômico. O produto do PCR foi digerido com a enzima de restrição CfoI, seguindo-se análise dos fragmentos por eletroforese em gel de agarose. Dentre os 179 animais analisados, 126 (70,0%) foram caracterizados como NN, 50 (28,0%) como Nn, e 3 (2,0%) caracterizados como nn. A freqüência de heterozigotos foi maior (P < 0,05) em animais da raça Landrace do que em animais da raça Large White. Nove animais da raça Pietrain foram classificados como heterozigotos e tres como homozigotos recessivos. Estes resultados podem estar relacionados com a incidência de PSS e PSE nestas raças, as quais são largamente utilizadas em programas de cruzamento. A utilização de folículo piloso como fonte de DNA genômico foi um método rápido e não-invasivo que viabiliza a execução da caracterização do gene hal

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“…In recessive homozygotes (nn) the RYR1 gene leads to PSS and the post-mortem manifestation of pale, soft and exudative pork (PSE). In heterozygosis (Nn) the hal gene produces lower carcass quality (BAND et al, 2005a;BASTOS et al, 2001;POMMIER & HOUDE, 1993), but possibly higher carcass weight (BAND et al, 2005b;LEACH et al, 1996). The polymorphism at nucleotide 1843 of the hal gene has been characterized by PCR amplification associated to restriction endonuclease assay (PCR-REA) using blood or muscle biopsy as the source of genomic DNA KAMINSKI et al, 2002).…”

Section: Uma Mutação No Gene Que Codifica O Receptor Ryanodine 1 (Ryrmentioning

confidence: 99%

An improved method for characterization of the mutation associated to porcine stress syndrome by PCR amplification followed by restriction analysis

et al. 2009

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Effect of the genotype for malignant hyperthermia as determined by a restriction endonuclease assay on the quality characteristics of commercial pork loins1

Cited by 56 publications

References 15 publications

Comparison of PRKAG3 and RYR1 gene effect on carcass traits and meat quality in Slovenian commercial pigs

Comparison of PRKAG3 and RYR1 gene effect on carcass traits and meat quality in Slovenian commercial pigs

Characterization of swine stress gene by DNA testing using plucked hair as a source of DNA

An improved method for characterization of the mutation associated to porcine stress syndrome by PCR amplification followed by restriction analysis

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