Effect of the Hemoregulatory Peptide (pEEDCK)2 (pyroGlu‐Glu‐Asp‐Cys‐Lys)2 and MIP‐1α is Reduced in Bone Marrow Cultures from Patients with Chronic Myeloid Leukemia (CML)

Karlic, H.; Louda, Norbert; Pfeilstöcker, Michael; Keil, Felix; Lohninger, Alfred; Pittermann, E.; Paukovits, Johanna B.

doi:10.1634/stemcells.19-4-321

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…Carnitinine palmitoyltransferase 1A (Cpt1a) is the key regulatory enzyme of hepatic long-chain fatty acid β-oxidation [25]. Cpt1A is also known to regulate apoptotic processes [26]. Gja1, also known as connexin 43, is a member of the larger family of connexins, the subunits of gap junctions, and it's mutation has been shown to cause skeletal malformations [27].…”

Section: Highly Expressed Genes and Pathways In Both Pc And Rzmentioning

confidence: 99%

Microarray analysis of perichondral and reserve growth plate zones identifies differential gene expressions and signal pathways

Zhang

Pritchard

Middleton

et al. 2008

Bone

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In the growth plate, the reserve and perichondral zones have been hypothesized to have similar functions, but their exact functions are poorly understood. Our hypothesis was that significant differential gene expression exists between perichondral and reserve chondrocytes that may differentiate the respective functions of these two zones. Normal Sprague-Dawley rat growth plate chondrocytes from the perichondral zone (PC) and reserve zone (RZ) were isolated by laser microdissection and then subjected to microarray analysis. In order to most comprehensively capture the unique features of the two zones, we analyzed both the most highly expressed genes and those that were most significantly different from the proliferative zone (PZ) as a single comparator. Confirmation of the differential expression of selected genes was done by quantitative real time RT-PCR. A total of 8 transcripts showing high expression unique to the PC included translationallycontrolled tumor protein (Tpt1), connective tissue growth factor (Ctgf), mortality factor 4 (Morf4l1), growth arrest specific 6 (Gas6), type V procollagen (Col5a2), frizzled-related protein (Frzb), GDP dissociation inhibitor 2 (Gdi2) and Jun D proto-oncogene (Jund). In contrast, 8 transcripts showing unique high expression in the RZ included hyaluronan and proteoglycan link protein 1 (Hapln1), hemoglobin beta-2 subunit, type I procollagen (Col1a2), retinoblastoma binding protein 4 (LOC685491), Sparc related modular calcium binding 2 (Smoc2), and calpastatin (Cast). Other genes were highly expressed in cells from both PC and RZ zones, including collagen II, collagen IX, catenin (cadherin associated protein) beta 1, eukaryotic translation elongation factor, high mobility group, ribosomal protein, microtubule-associated protein, reticulocalbin, thrombospondin, retinoblastoma binding protein, carboxypeptidase E, carnitine palmitoyltransferase 1, cysteine rich glycoprotein, plexin B2 (Plxnb2), and gap junction membrane channel protein. Functional classification of the most highly expressed transcripts were analyzed, and the pathway analysis indicated that ossification, bone remodeling, and cartilage development were uniquely enriched in the PC whereas both the PC and RZ showed pathway enrichment for skeletal development, extracellular matrix structural constituent, proteinaceous extracellular matrix, collagen, extracellular matrix, and extracellular matrix part pathways. We conclude that differential gene expression exists between the RZ and PC

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Section: Highly Expressed Genes and Pathways In Both Pc And Rzmentioning

confidence: 99%

Microarray analysis of perichondral and reserve growth plate zones identifies differential gene expressions and signal pathways

Zhang

Pritchard

Middleton

et al. 2008

Bone

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“…Quantitative reverse transcriptase polymerase chain reaction (rtRT-PCR) (Weghofer et al 2001;Karlic et al 2001: Karlic et al 2002 was carried out using the LightCycler System (Roche) which allows amplification and detection. Dilutions of 200-2 ng of cDNA were used in each assay.…”

Section: Analysis Of Mrna Expressionmentioning

confidence: 99%

Do blood cells mimic gene expression profile alterations known to occur in muscular adaptation to endurance training ?

et al. 2005

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Exercise is known to upregulate mRNA synthesis for carnitine palmitoyl transferase1 (CPT1) and possibly also other mitochondrial carnitine acyltransferases in muscle tissue. The aim of this study was to test whether such an adaptation of oxidative metabolism in skeletal muscle is a systemic process and consequently, also affects other cells. Messenger RNA levels of five genes [carnitine palmitoyl transferases 1 and 2 (CPT1 and CPT2), carnitine acetyltransferase (CRAT), carnitine palmitoyltransferase 2 (CPT2), microsomal carnitine palmitoyltransferase (GRP58) and organic cation transporter (OCTN2)] were determined with quantitative real time polymerase chain reaction (PCR) in blood cells and in muscle biopsy samples from six cross country skiers before and 6 months after a high volume/low intensity exercise training, when training had elicited a significantly slower rate of lactate accumulation. Quantitative real time PCR showed that levels of mRNA in blood cells correlated significantly (CPT1B: P< 0.001) with those in muscle tissue from the same donors. After 6-months training, there was a 15-fold upregulation of CPT1B mRNA, a six to ninefold increase of CRAT mRNA, of CPT2 mRNA, GRP58 mRNA, and of OCTN2 mRNA. The observation of a concordant stimulation of CPT1, CPT2, CRAT, GRP58 and OCTN2 transcription in blood cells and muscle tissue after 6-month-endurance training leads the hypothesis of a common stimulation mechanism other than direct mechanical stress or local chemical environment, but rather humoral factors.

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Downregulation of carnitine acyltransferases and organic cation transporter OCTN2 in mononuclear cells in healthy elderly and patients with myelodysplastic syndromes

Karlic

Lohninger

Laschan

et al. 2003

Journal of Molecular Medicine

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Changes in key enzymes of oxidative metabolism at the mitochondrial level are known to be associated with the aging process, apoptosis, and many diseases. Considering the risk of acquiring a myelodysplastic syndrome (MDS) with age, the aim of this study was to quantify mRNA synthesis of the carnitine palmitoyltransferases (CPT1 and CPT2), carnitine acetyltransferase (CRAT), human specific microsomal CPT, and OCTN2 (organic cation transporter) in mononuclear cells of healthy humans of different age groups and MDS patients. Using quantitative reverse transcriptase real-time PCR we compared mRNA synthesis of the above mentioned enzymes in mononuclear cells from peripheral blood of 23 healthy persons (mean age 45 years), 9 blood and 22 bone marrow samples of 31 MDS patients with varying proportions of apoptotic cells (mean age 78 years), and blood samples of 30 age-matched controls. In addition, plasma carnitine levels were determined. Compared to younger adults, there was a 50% downregulation of CPT1 in elderly persons and in MDS patients. Reduction in CRAT, CPT 2, and OCTN2 was more than 85%. Reduction in microsomal CPT was more pronounced in MDS patients than in age-matched controls (96% vs. 43%). In MDS bone marrow cells there was a negative correlation of CPT1 and CRAT with the relative proportion of apoptotic cells. Plasma carnitine values were similar in all groups. The described reduction in transcription of different genes in blood cells which is well known in different tissues may reflect a systemic signaling process, associated with aging, apoptosis, and MDS.

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Effect of the Hemoregulatory Peptide (pEEDCK)2 (pyroGlu‐Glu‐Asp‐Cys‐Lys)2 and MIP‐1α is Reduced in Bone Marrow Cultures from Patients with Chronic Myeloid Leukemia (CML)

Cited by 6 publications

References 27 publications

Microarray analysis of perichondral and reserve growth plate zones identifies differential gene expressions and signal pathways

Microarray analysis of perichondral and reserve growth plate zones identifies differential gene expressions and signal pathways

Do blood cells mimic gene expression profile alterations known to occur in muscular adaptation to endurance training ?

Downregulation of carnitine acyltransferases and organic cation transporter OCTN2 in mononuclear cells in healthy elderly and patients with myelodysplastic syndromes

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