“…However, compared to small‐scale tests, these instrumental methods of analysis are labor‐intensive, time‐consuming, and expensive (Ram, Dawar, Singh, & Shoran, ) and use relatively large amounts of flour (Chandi & Seetharaman, ; Karaduman, Önder, Sayaslan, & Aydın, ). Even though GlutoPeak is a rapid test for measuring gluten aggregation (Melnyk, Dreisoerner, Bonomi, Marcone, & Seetharaman, ; Marti, Ulrici, Foca, Quaglia, & Pagani, ; Fu, Wang, & Dupuis, ), it requires at least 8.5 g of whole‐wheat flour (Karaduman et al, ), and the test time is nearly 10 min per sample. It is necessary for rapid and small‐scale methods to screen a great many genotypes in the initial breeding stages (Li et al, ).…”