Effect of the Lippia alba (Mill.) N.E. Brown essential oil and its main constituents, citral and limonene, on the tracheal smooth muscle of rats

Carvalho, Poliana M.M.; Macêdo, Cícero André Ferreira; Ribeiro, Tiago F.; Silva, Andressa A.; Silva, Renata E.R. Da; Pereira-de-Morais, Luís; Kerntopf, Marta Regina; Menezes, Irwin Rose Alencar de; Barbosa, Roseli

doi:10.1016/j.btre.2017.12.002

Cited by 27 publications

(15 citation statements)

References 16 publications

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“…Similar to our results, several monoterpenes have been suggested to block VOCCs in smooth muscles from various rat organs; these include citral in the aorta [31], 1,8-cineole, citral and limonene in the trachea [42,43], as well as citral and limonene in the uterus [44]. In addition, endogenous substances, such as progesterone and 5β-pregnanediol, have shown that VOCC inhibition is the main vascular relaxation route stimulated by these hormones in HUA vessels [33].…”

Section: Discussionsupporting

confidence: 89%

Relaxant Effect of Monoterpene (−)-Carveol on Isolated Human Umbilical Cord Arteries and the Involvement of Ion Channels

Silva

Pereira-de-Morais

et al. 2020

Molecules

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Carveol is a monoterpene present in the structure of many plant products. It has a variety of biological activities: antioxidant, anticancer and vasorelaxation. However, studies investigating the effect of monoterpenoids on human vessels have not yet been described. Thus, the present study aimed to characterize the effect of (−)-carveol on human umbilical arteries (HUAs). HUA ring preparations were isolated and subjected to isometric tension recordings of umbilical artery smooth muscle contractions. (−)-Carveol exhibited a significant vasorelaxant effect on KCl and 5-HT-induced contractions, obtaining EC50 values of 344.25 ± 8.4 and 175.82 ± 4.05 µM, respectively. The participation of calcium channels in the relaxation produced by (−)-carveol was analyzed using vessels pre-incubated with (−)-carveol (2000 µM) in a calcium-free medium, where the induction of contractions was abolished. The vasorelaxant effect of (−)-carveol on HUAs was reduced by tetraethylammonium (TEA), which increased the (−)-carveol EC50 to 484.87 ± 6.55 µM. The present study revealed that (−)-carveol possesses a vasorelaxant activity in HUAs, which was dependent on the opening of calcium and potassium channels. These results pave the way for further studies involving the use of monoterpenoids for the vasodilatation of HUAs. These molecules have the potential to treat diseases such as pre-eclampsia, which is characterized by resistance in umbilical arteries.

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Section: Discussionsupporting

confidence: 89%

Relaxant Effect of Monoterpene (−)-Carveol on Isolated Human Umbilical Cord Arteries and the Involvement of Ion Channels

Silva

Pereira-de-Morais

et al. 2020

Molecules

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“…In this model, contraction occurs through the binding of the agonist (PGF2α) to its receptor, also called prostanoid receptors or G protein-coupled receptors, that activates phospholipase C (PLC), and leads to an increase in intracellular Ca 2+ level and activation of protein kinase C (PKC), phosphatidylinositol-3-kinase (PI3K) and ERK1/2 in the human myometrium (Heppelmann et al, 2018;Xu et al, 2015). Considering reports in the literature, citral also can inhibit the contractions of the tracheal smooth muscle of rats by reduction of Ca 2+ influx (Carvalho et al, 2018); in fact, some chemical compounds from essential oils, such as citral, geranial or lemongrass, have been shown to have vascular relaxant effects in different experimental models of cardiovascular diseases, such property has been related to the blockade of Ca 2+ influx (de Andrade, Brasil, Endringer, da Nobrega, & de Sousa, 2017). These data are consistent with our results, citral also can inhibit PGF2α-induced contractions in the human myometrium, an effect which could also be related to the blockade of the calcium channels, as reported in the studies mentioned above.…”

Section: Discussionmentioning

confidence: 99%

In vitro effects of citral on the human myometrium: Potential adjunct therapy to prevent preterm births

Muñoz-Pérez

Ortiz

Salas-Casa

et al. 2021

Birth Defects Research

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Background: Premature infants contribute to infant morbidity and mortality especially in low resource settings. Information on tocolytic and/or antiinflammatory effects of several plant extracts, such as citral, could help prevent preterm birth cases and reduce the number of preterm infants. The aim of this study was to evaluate the in vitro tocolytic and anti-inflammatory effect of citral on myometrial tissues of the human uterus.Methods: Myometrial samples from uteri obtained after hysterectomy were used in functional tests to evaluate the inhibitory effect of citral on PGF-2α induced contractions. The intracellular cyclic adenosine monophosphate (cAMP) levels generated in response to citral in human myometrial homogenates were measured by ELISAs. Forskolin was used as a positive control. The anti-inflammatory effect of citral was determined through the measurement of two pro-inflammatory cytokines, tumor necrosis factor-α (TNFα) and interleukin (IL)-1β, and the anti-inflammatory cytokine IL-10, in human myometrial explants stimulated with lipopolysaccharide (LPS).Results: Citral was able to induce a significant inhibition of PGF-2α induced contractions at the highest concentration level (p < .05). Citral caused a concentration-dependent increase in myometrial cAMP levels (p < .05) and a concentration-dependent decrease in LPS-induced TNFα and IL-1β production, while IL-10 production increased significantly (p < .05). The antiinflammatory and tocolytic effects induced by citral could be associated with an increase in cAMP levels in human myometrial samples. Conclusion:These properties place citral as a potentially safe and effective adjuvant agent in preterm birth cases, an obstetric and gynecological problem that requires urgent attention.

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“…The pharmacological potential of Lippia alba essential oils has been widely described in the literature [13,17,32]; however, its action on Gram-negative bacteria of medical importance has been little explored. The present study demonstrated the excellent antimicrobial potential of this oil on strains causing gastrointestinal infections.…”

Section: Discussionmentioning

confidence: 99%

Modulatory Effect of Lippia alba Essential Oil on the Activity of Clinically Used Antimicrobial Agents on Salmonella typhi and Shigella dysenteriae Biofilm

et al. 2018

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The essential oil obtained from the leaves of Lippia alba (Mill.) N.E. Brown (Verbenaceae) has shown great pharmacological potential as an analgesic, antispasmodic, and antimicrobial agent. The aim of this study was to evaluate the modulatory effect of Lippia alba essential oil (LaEO I) on the activity of clinically used antimicrobial agents on Salmonella enterica serovar Typhi (Salmonella typhi) and Shigella dysenteriae biofilms. The Minimum Inhibitory Concentration of LaEO I (MICLaEO I) was determined by the microdilution method, and the effect of LaEO I on the activity of clinically used antimicrobials was assessed by the Checkboard method. The values obtained from MICLaEO I and ciprofloxacin were used to evaluate the effect of time of exposure on cell viability. LaEO I main components were geranial (34.2%), neral (25.9%), and myrcene (12.5%). The MICLaEO I was 1 mg/mL for both strains. LaEO I positively modulated the action of ciprofloxacin, cefepime, and ceftriaxone. After the first hour of treatment with MICLaEO I, the cell viability of the strains showed a 5 log10 CFU/mL reduction, and the LaEO I-CIP association was able to inhibit growth during the first 6 h of the test. Regarding the anti-biofilm activity, MICLaEO I was able to reduce the biofilm mass of Salmonella typhi by 61.2% and of Shigella dysenteriae by 38.9%. MICLaEO I was not able to eradicate the preformed biofilm; however, there was a reduction in the biofilm microbial viability. LaEO I has the potential to be used as an antimicrobial agent and interferes with biofilm formation; also, it is able to reduce cell viability in preformed biofilm and synergistically modulate the activity of ciprofloxacin.

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Effect of the Lippia alba (Mill.) N.E. Brown essential oil and its main constituents, citral and limonene, on the tracheal smooth muscle of rats

Cited by 27 publications

References 16 publications

Relaxant Effect of Monoterpene (−)-Carveol on Isolated Human Umbilical Cord Arteries and the Involvement of Ion Channels

Relaxant Effect of Monoterpene (−)-Carveol on Isolated Human Umbilical Cord Arteries and the Involvement of Ion Channels

In vitro effects of citral on the human myometrium: Potential adjunct therapy to prevent preterm births

Modulatory Effect of Lippia alba Essential Oil on the Activity of Clinically Used Antimicrobial Agents on Salmonella typhi and Shigella dysenteriae Biofilm

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