Effect of the mi allele on mast cells, basophils, natural killer cells, and osteoclasts in C57BI/6J mice

Stechschulte, Daniel J.; Sharma, Ram Kumar; Dileepan, Kottarappat N.; Simpson, Karen M.; Aggarwal, Neelam; Clancy, John; Jilka, Robert L.

doi:10.1002/jcp.1041320321

Cited by 105 publications

(53 citation statements)

References 29 publications

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“…PU.1, GATA-2, GATA-3, and the microphthalmia-associated transcription factor are essential for mast cell development [22][23][24][25]. We showed that adiposederived CFCs express these four transcription factors as peritoneal cell-derived mast cell controls (Fig.…”

Section: Adipose Tissue Harbors Mast Cell Progenitors and Precursorsmentioning

confidence: 89%

Adipose Tissue as a Dedicated Reservoir of Functional Mast Cell Progenitors

et al. 2010

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White adipose tissue (WAT) is a heterogeneous tissue, found in various locations throughout the body, containing mature adipocytes and the stroma‐vascular fraction (SVF). The SVF includes a large proportion of immune hematopoietic cells, among which, mast cells that contribute to diet‐induced obesity. In this study, we asked whether mast cells present in mice adipose tissue could derive from hematopoietic stem/progenitor cells (HSPC) identified in the tissue. We therefore performed both in vitro and in vivo experiments dedicated to monitoring the progeny of WAT‐derived HSPC. The entire study was conducted in parallel with bone marrow‐derived cells, considered the gold standard for hematopoietic‐lineage studies. Here, we demonstrate that adipose‐derived HSPC contain a precursor‐cell population committed to the mast cell lineage, and able to efficiently home to peripheral organs such as intestine and skin, where it acquires properties of functional tissue mast cells. Additionally, WAT contains a significant mast cell progenitor population, suggesting that the entire mast cell lineage process take place in WAT. Considering the quantitative importance of WAT in the adult organism and the increasing roles recently assigned to mast cells in physiopathology, WAT may represent an important source of mast cells in physiological and pathological situations. STEM CELLS 2010;28:2065–2072

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Section: Adipose Tissue Harbors Mast Cell Progenitors and Precursorsmentioning

confidence: 89%

Adipose Tissue as a Dedicated Reservoir of Functional Mast Cell Progenitors

et al. 2010

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“…MITF is expressed in melanocytes, in retina pigment cells, in mast cells and in osteoclasts (50,51). Pigmentation defects in mi mice seem to be a result of the absence of melanocytes.…”

Section: Camp-dependent Intracellular Pathways Involved In the Regulamentioning

confidence: 99%

Cyclic AMP a Key Messenger in the Regulation of Skin Pigmentation

Buscà

Ballotti

2000

Pigment Cell Research

734

640

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In humans, stimulation of skin pigmentation over the basal constitutive level, which we commonly call tanning, is physiologically stimulated by ultraviolet (UV) radiation of the solar light. UV-induced skin darkening involves an increase in the melanocyte number as well as a stimulation of melanin neosynthesis and melanocyte dendricity, a crucial morphological feature required for melanin transfer to keratinocytes. These events, corresponding to the final steps of melanocyte differentiation, play a central role in the tanning response and are subjected to an accurate research which aims to understand the precise mechanisms governing their regulation. In the present review, we will mainly focus our attention on the molecular processes involved in the regulation of melanogenesis.

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“…The mi-MITF is defective in the DNA binding activity and the nuclear localization potential, and it does not transactivate target genes Takebayashi et al, 1996;Tsujimura et al, 1996;Jippo et al, 1997;Ito et al, 1998;Kim et al, 1998). The mi/mi mice show microphthalmia, depletion of pigment in both hair and eyes, osteopetrosis, and decrease of mast cells (Silvers, 1979;Green, 1981;Stevens and Loutit, 1982;Stechschulte et al, 1987). In addition to the decrease in number, the phenotype of mast cells is abnormal in mi/mi mice (Ebi et al, 1990(Ebi et al, , 1992Kasugai et al, 1993;Isozaki et al, 1994;Jippo et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Synergy of PEBP2/CBF with mi transcription factor (MITF) for transactivation of mouse mast cell protease 6 gene

et al. 1999

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The mi locus encodes a member of the basic ± helix ± loop ± helix ± leucine zipper (bHLH-Zip) protein family of transcription factors (hereafter called MITF). Although the bHLH-Zip family transcription factors generally recognize and bind CANNTG motifs, the expression of mouse mast cell protease 6 (MMCP-6) gene is regulated by MITF through the GACCTG motif in the promoter region. The GACCTG motif was partly overlapped the TGTGGTC sequence, which was bound by polyomavirus enhancer binding protein 2 (PEBP2). In the present study, the eect of PEBP2 on the expression of MMCP-6 gene was examined. PEBP2 that is composed of a and b subunits was expressed by mast cell lines and cultured mast cells derived from spleen. The overexpression of dominant negative PEBP2 cDNA reduced the expression of MMCP-6. Moreover, the simultaneous transfection of the plasmid containing MITF cDNA and the plasmid containing PEBP2 cDNA increased the MMCP-6 promoter activity. For the synergistic action of PEBP2 and MITF, the intact GACCTG and TGTGGTC motifs were prerequisite. The PEBP2aB1 mutant which lacked the region downstream from the Runt domain did not bind MITF and lost the synergistic function. These results indicated that PEBP2 and MITF synergistically transactivated the MMCP-6 gene and that the region downstream from the Runt domain of PEBP2aB1 was essential for the physical and functional interactions with MITF.

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Effect of the mi allele on mast cells, basophils, natural killer cells, and osteoclasts in C57BI/6J mice

Cited by 105 publications

References 29 publications

Adipose Tissue as a Dedicated Reservoir of Functional Mast Cell Progenitors

Adipose Tissue as a Dedicated Reservoir of Functional Mast Cell Progenitors

Cyclic AMP a Key Messenger in the Regulation of Skin Pigmentation

Synergy of PEBP2/CBF with mi transcription factor (MITF) for transactivation of mouse mast cell protease 6 gene

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