Effect of the mixture of mulberry leaf powder and KGM flour on promoting calcium absorption and bone mineral density in vivo

Abstract

BACKGROUND In this paper, mulberry leaf powder (MLP) and konjac glucomannan (KGM) flour were used as raw materials, and animal experiments were designed to evaluate the effects of a mixture of MLP and KGM on bone density. The femoral bone microstructure of mice and pathological changes were observed by using micro‐computed tomography) and haematoxylin and eosin (HE) staining methods, respectively. A three‐point bending test was used to determine the biomechanical properties of the femur. RESULTS Results indicated that the calcium content of MLP was high, reaching 16 148.5 mg kg−1, and the total proportion of water‐soluble calcium, calcium pectinate, and calcium carbonate accounted for about 60% of the total calcium content. Serum alkaline phosphatase (AKP) activity was significantly lower, and serum calcium content was significantly higher (P < 0.05), in the MLP + KGM group (KM) than in the low‐calcium control group, whereas no significant difference (P > 0.05) was found for serum phosphorus content. KM had a longer femur length, a higher bone mineral density (BMD) (P > 0.05), and significantly greater femur diameter, dry weight, index and bone calcium content (P < 0.05). However, these parameters were not significantly different from those of the calcium carbonate control group (P > 0.05). CONCLUSION The results indicate that the MLP/KGM mixture can reduce the high rate of bone turnover and the corresponding loss of bone mass caused by calcium deficiency and is thus effective in enhancing bone density. © 2019 Society of Chemical Industry

Mulberry leaf and konjac compound powder improves the metabolic capacity of old mice on a high‐protein diet by regulating the structure of the intestinal microbiota

“…However, excess protein may also have a negative feedback regulation on enzyme secretion and activity. This regulation is related to the abundance of excess protein and animals of different types 38 . It could be inferred that the opposite result might be attributed to the significant increase in the transcription level of genes related to protein metabolism under high‐protein concentration, followed by negative feedback regulation of the translation level.…”

“…This regulation is related to the abundance of excess protein and animals of different types. 38 It could be inferred that the opposite result might be attributed to the significant increase in the transcription level of genes related to protein metabolism under high-protein concentration, followed by negative feedback regulation of the translation level. Alternatively, it is plausible that protein degradation occurred during the synthesis process, leading to a decrease in the translation level of relevant genes, as reported by Wu.…”

Preparation of synbiotic milk powder and its effect on calcium absorption and the bone microstructure in calcium deficient mice

“…The body weight of the mice was measured once a week using an electronic scale, each time after fasting the mice for 12 h. The body length was measured using vernier calipers. 32…”

Mulberry leaf and konjac flour compound dietary fiber improves digestion and metabolism in elderly mice with high-fish-protein diet by regulating gut microbiota structure and intestinal tissue repair

“…Konjac glucomannan (KGM), the main component of konjac flour, is a water-soluble dietary fiber known for its high viscosity and capacity to improve intestinal health and bolster the immune system ( Zhang et al, 2021 ; Zhang et al, 2022 ). Animal studies have demonstrated that the compound dietary fiber composed of mulberry leaf and konjac flour (CMK) can reduce the pH of the intestine, elevate the content of short-chain fatty acids (SCFA) ( Deng, Luo, Zhang, & Zhong, 2020 ) and enhance beef protein metabolism in mice consuming a high in beef-protein diet ( Deng, Zhong, Peng, & Zhu, 2023 ). In contrast to beef, fish meat is an ideal source of complete protein for the elderly due to its low-fat content, high-protein levels, and essential amino acid composition.…”