Effect of the molecular weight of polymer and diluent on the performance of hydrophilic poly(vinyl butyral) porous heddle via thermally induced phase separation

Luo, Yang; Yang, Fan; Li, Chengcai; Wang, Feng; Zhu, Hailin; Guo, Yuhai

doi:10.1016/j.matchemphys.2021.124227

Cited by 7 publications

(4 citation statements)

References 36 publications

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“…Antibody-conjugated PicoShells may be used to produce hypersecreting and hypergrowing CHO cell populations based on their behavior in bioreactors for scaled production of protein therapeutics. The pore size of the particles may also be modulated by changing the molecular weight (MW) of PEG used to cross-link the solid phase ( 55 ) or by including nonfunctionalized PEG ( 56 ), gelatin ( 57 ), or hyaluronic acid ( 58 ) in the PEG phase. Adherence motifs, such as arginine-glycine-aspartate (RGD) peptides, fibronectin, or poly- l -lysine, may be also added to the outer PEG matrix so that stem cells, adherent CHO cells, or other adherent cell types have a solid surface to adhere to, further expanding the potential applications of the PicoShell workflow.…”

Section: Discussionmentioning

confidence: 99%

High-throughput selection of cells based on accumulated growth and division using PicoShell particles

Zee

Rutte

Rumyan

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Production of high-energy lipids by microalgae may provide a sustainable energy source that can help tackle climate change. However, microalgae engineered to produce more lipids usually grow slowly, leading to reduced overall yields. Unfortunately, culture vessels used to select cells based on growth while maintaining high biomass production, such as well plates, water-in-oil droplet emulsions, and nanowell arrays, do not provide production-relevant environments that cells experience in scaled-up cultures (e.g., bioreactors or outdoor cultivation farms). As a result, strains that are developed in the laboratory may not exhibit the same beneficial phenotypic behavior when transferred to industrial production. Here, we introduce PicoShells, picoliter-scale porous hydrogel compartments, that enable >100,000 individual cells to be compartmentalized, cultured in production-relevant environments, and selected based on growth and bioproduct accumulation traits using standard flow cytometers. PicoShells consist of a hollow inner cavity where cells are encapsulated and a porous outer shell that allows for continuous solution exchange with the external environment. PicoShells allow for cell growth directly in culture environments, such as shaking flasks and bioreactors. We experimentally demonstrate that Chlorella sp., Saccharomyces cerevisiae, and Chinese hamster ovary cells, used for bioproduction, grow to significantly larger colony sizes in PicoShells than in water-in-oil droplet emulsions (P < 0.05). We also demonstrate that PicoShells containing faster dividing and growing Chlorella clonal colonies can be selected using a fluorescence-activated cell sorter and regrown. Using the PicoShell process, we select a Chlorella population that accumulates chlorophyll 8% faster than does an unselected population after a single selection cycle.

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Section: Discussionmentioning

confidence: 99%

High-throughput selection of cells based on accumulated growth and division using PicoShell particles

Zee

Rutte

Rumyan

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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“…Recently, diverse solution-based techniques have been combined to obtain a 3D porous structure with interconnected pores for TE applications. The TIPS technique is a commonly used method to fabricate TE scaffolds due to its simplicity and effectiveness in adjusting the structure and properties to the application of materials [70,74]. Carfì et al [101] combined TIPS and DIPS to produce a multifunctional scaffold with easy-tunable pore size, porosity, and thickness.…”

Section: Combination Of Solution-based Techniquesmentioning

confidence: 99%

“…The mechanical performances and morphological properties have a significant relationship in the scaffold fabrication process. Luo et al [ 70 ] produced scaffolds with different porosities and microstructures using TIPS to study the relationship between the polymer average molecular weight and tensile strength. In the study of Sabzi et al [ 18 ], desirable mechanical properties and the control over fiber diameter and orientation of nanofibrous gelatin scaffolds were obtained by combining TIPS with the particulate leaching technique.…”

Section: Phase Separationmentioning

confidence: 99%

Solution-Based Processing for Scaffold Fabrication in Tissue Engineering Applications: A Brief Review

et al. 2021

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The fabrication of 3D scaffolds is under wide investigation in tissue engineering (TE) because of its incessant development of new advanced technologies and the improvement of traditional processes. Currently, scientific and clinical research focuses on scaffold characterization to restore the function of missing or damaged tissues. A key for suitable scaffold production is the guarantee of an interconnected porous structure that allows the cells to grow as in native tissue. The fabrication techniques should meet the appropriate requirements, including feasible reproducibility and time- and cost-effective assets. This is necessary for easy processability, which is associated with the large range of biomaterials supporting the use of fabrication technologies. This paper presents a review of scaffold fabrication methods starting from polymer solutions that provide highly porous structures under controlled process parameters. In this review, general information of solution-based technologies, including freeze-drying, thermally or diffusion induced phase separation (TIPS or DIPS), and electrospinning, are presented, along with an overview of their technological strategies and applications. Furthermore, the differences in the fabricated constructs in terms of pore size and distribution, porosity, morphology, and mechanical and biological properties, are clarified and critically reviewed. Then, the combination of these techniques for obtaining scaffolds is described, offering the advantages of mimicking the unique architecture of tissues and organs that are intrinsically difficult to design.

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“…Such fabrics have many advantages such as washability, abrasion resistance high strength, dimensional stability, wrinkle resistance, attractive handling, and stretch resistance. On the other hand, some unwanted features have emerged such as the accumulation of static electric charge and a lack of hydrophilicity due to a poor moisture recovery capacity (0.4 percent) [1][2][3][4][5]. Polyester has been widely used as an apparel and technical textile material in the form of fabrics, films, and plastics due to its excellent mechanical and physical properties [6,7].…”

Section: Introductionmentioning

confidence: 99%

Prewetting Induced Hydrophilicity to Augment Photocatalytic Activity of Nanocalcite @ Polyester Fabric

et al. 2022

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To eliminate imidacloprid insecticide from wastewater, nanocalcite was grafted onto the surface of pretreated polyester fabric. The process of seeding was followed by the low temperature hydrothermal method for the growth of nanocalcite for the functionalization of fabric. The goal of this study was to improve the hydrophilicity of the nanocalcite photocatalyst that had been grafted onto the surface of polyester fabric (PF) using acidic and basic prewetting techniques. The morphological characteristics, crystalline nature, surface charge density, functional groups of surface-modified nanocalcite @ PF were determined via SEM, XRD, FTIR, and Zeta potential (ZP), respectively. Characterization results critically disclosed surface roughness due to excessive induction of hydroxyl groups, rhombohedral crystal structure, and high charge density (0.721 mS/cm). Moreover, contact angle of nanocalcite @ PF was calculated to be 137.54° while after acidic and basic prewetting, it was reduced to 87.17° and 48.19°. Similarly, bandgap of the as fabricated nanocalcite was found to be 3.5 eV, while basic prewetted PF showed a reduction in band gap (2.9 eV). The solar photocatalytic mineralization of imidacloprid as a probe pollutant was used to assess the improvement in photocatalytic activity of nanocalcite @ PF after prewetting. Response surface methodology was used to statistically optimize the solar exposure time, concentration of the oxidant, and initial pH of the reaction mixture. Maximum solar photocatalytic degradation of the imidacloprid was achieved by basic prewetted nanocalcite @ PF (up to 91.49%), which was superior to acidic prewetted fabric and as-fabricated nanocalcite @ PF. Furthermore, HPLC and FTIR findings further indicated that imidacloprid was decomposed vastly to harmless species by basic prewetted nanocalcite @ PF.

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Effect of the molecular weight of polymer and diluent on the performance of hydrophilic poly(vinyl butyral) porous heddle via thermally induced phase separation

Cited by 7 publications

References 36 publications

High-throughput selection of cells based on accumulated growth and division using PicoShell particles

High-throughput selection of cells based on accumulated growth and division using PicoShell particles

Solution-Based Processing for Scaffold Fabrication in Tissue Engineering Applications: A Brief Review

Prewetting Induced Hydrophilicity to Augment Photocatalytic Activity of Nanocalcite @ Polyester Fabric

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