Effect of the Nitrogen Source on Phycobiliprotein Synthesis and Cell Reserves in A Chromatically Adapting Filamentous Cyanobacterium

Liotenberg, Sylviane; Campbell, Douglas A.; Rippka, Rosmarie; Houmard, Jean; Marsac, Nicole Tandeau de

doi:10.1099/13500872-142-3-611

Cited by 99 publications

(74 citation statements)

References 60 publications

(81 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cyanobacteria are a diverse group of photosynthetic microorganisms with specific physiological and morphological characteristics that allow them to adapt to a wide range of habitats including pelagic and benthic environments where they are highly competitive (Liotenberg et al, 1996;Morvan et al, 1997;Oren, 2000). Some species can survive under extreme environmental conditions such as deserts, hot springs and alkaline lakes (Oren, 2000).…”

Section: Introductionmentioning

confidence: 99%

Potentially harmful cyanobacteria in oyster banks of Términos lagoon, southeastern Gulf of Mexico

Poot-Delgado¹,

Okolodkov²,

Aké-Castillo³

et al. 2018

Acta biol. Colomb.

View full text Add to dashboard Cite

Cyanobacteria inhabit hypersaline, marine and freshwater environments. Some toxic and non-toxic species can form harmful blooms. The aim of this study was to identify potentially harmful cyanobacterial species in the oyster banks of Términos Lagoon, the southeastern Gulf of Mexico. Six sample sites (up to 2-m depth) were monitored monthly from August 2012 to September 2013. Water temperature, salinity, pH, dissolved oxygen saturation (% DO), inorganic nutrients and abundance of cyanobacteria were determined. Temperature and salinity were characterized by marked seasonal differences (26.8 to 30.6 °C and 6.1 to 19.5, respectively). The pH values (ranging from 7.1 to 8.4) and the % DO (88.4 to 118.2 %) suggest a predominance of photosynthetic activity in the windy season (October-February). Elevated nutrient contents are associated with the period of increased river discharge, determined by water circulation and biogeochemical processes. Fourteen taxa were identified, of which Anabaena sp., Merismopedia sp., Oscillatoria sp. and Cylindrospermopsis cuspis produced blooms. Cyanobacterial abundances were on the order of magnitude of 10 6 cells L -1 in October 2012 at stations S1-S6, with an average value of 3.2x10 5 cells L -1 and a range of 2000 to 3.1x10 6 cells L -1 throughout the study period; however, they showed a remarkable absence during the windy season (October to January). Anabaena sp. and C. cuspis reached abundances of 1.9x10 6 and 1.3x10 6 cells L -1, respectively. The latter caused the temporary closure of oyster Crassostrea virginica harvesting for 15 days in October 2012.

show abstract

Section: Introductionmentioning

confidence: 99%

Potentially harmful cyanobacteria in oyster banks of Términos lagoon, southeastern Gulf of Mexico

Poot-Delgado¹,

Okolodkov²,

Aké-Castillo³

et al. 2018

Acta biol. Colomb.

View full text Add to dashboard Cite

show abstract

“…most cyanobacteria as nitrogen, carbon, and energy storage compound in the early stationary growth phase (1,2). The water-insoluble CGP is accumulated intracellularly in the form of membraneless granules (3) and is degraded by the cells when growth is resumed.…”

mentioning

confidence: 99%

Isolation of Cyanophycin-degrading Bacteria, Cloning and Characterization of an Extracellular Cyanophycinase Gene (cphE) from Pseudomonas anguilliseptica Strain BI

Obst¹,

Oppermann‐Sanio²,

Luftmann³

et al. 2002

Journal of Biological Chemistry

View full text Add to dashboard Cite

Eleven bacteria capable of utilizing cyanophycin (cyanophycin granule polypeptide (CGP)) as a carbon source for growth were isolated. One isolate was taxonomically affiliated as Pseudomonas anguilliseptica strain BI, and the extracellular cyanophycinase (CphE) was studied because utilization of cyanophycin as a carbon source and extracellular cyanophycinases were hitherto not described. CphE was detected in supernatants of CGP cultures and purified from a corresponding culture of strain BI employing chromatography on the anion exchange matrix Q-Sepharose and on an arginineagarose affinity matrix. The mature form of the inducible enzyme consisted of one type of subunit with M r ‫؍‬ 43,000 and exhibited high specificity for CGP, whereas proteins and synthetic polyaspartic acid were not hydrolyzed or were only marginally hydrolyzed. Degradation products of the enzyme reaction were identified as aspartic acid-arginine dipeptides (␤-Asp-Arg) by high performance liquid chromatography and electrospray ionization mass spectrometry. The corresponding gene (cphE, 1254 base pairs) was identified in subclones of a cosmid gene library of strain BI by heterologous active expression in Escherichia coli, and its nucleotide sequence was determined. The enzyme exhibited only 27-28% amino acid sequence identity to intracellular cyanophycinases occurring in cyanobacteria. Analysis of the amino acid sequence of cphE revealed a putative catalytic triad consisting of the motif GXSXG plus a histidine and most probably a glutamate residue. In addition, the strong inhibition of the enzyme by Pefabloc ® and phenylmethylsulfonyl fluoride indicated that the catalytic mechanism of CphE is related to that of serine type proteases. Quantitative analysis on the release of ␤-Asp-Arg dipeptides from C-terminal labeled CGP gave evidence for an exo-degradation mechanism.Cyanophycin (cyanophycin granule polypeptide, CGP) 1 is a naturally occurring poly(amino acid), which is synthesized in most cyanobacteria as nitrogen, carbon, and energy storage compound in the early stationary growth phase (1, 2). The water-insoluble CGP is accumulated intracellularly in the form of membraneless granules (3) and is degraded by the cells when growth is resumed. The backbone of this unique biopolymer consists of ␣-amino-␣-carboxyl-linked L-aspartic acid monomers. Most of the ␤-carboxylic groups are covalently bound to the ␣-amino groups of L-arginine residues (4, 5); in recombinant Escherichia coli expressing cyanobacterial CGP-synthesizing enzymes (see below), a significant fraction of arginine is replaced by lysine (6).Although much information has been obtained concerning the non-ribosomal biosynthesis of CGP, which is catalyzed by the cyanophycin synthetase (CphA; see Ref. 7 and cited references therein), only a few reports are available on the intracellular degradation of CGP. Intracellular CGP degradation was first observed in crude extracts of soluble proteins prepared from cells of Anabaena cylindrica (5). The corresponding enzyme, cyanophycinase (CphB), was...

show abstract

“…The correlations between nitrogen concentration and cells growth as well as on the pigment production were well documented. Nitrogen is an essential major element required for the synthesis of primary and secondary amino acids, proteins, nucleic acids, coenzymes, chlorophyll and other accessory photosynthetic pigment such as phycobiliproteins in cyanobacteria [5]. It was reported that the concentration of KNO 3 as nitrogen source was found to be an essential factor influencing the growth of Synechocystis sp.…”

Section: Discussionmentioning

confidence: 99%

“…In many papers, the effects of nutrient, light, pH, temperature and co-cultivation with competitive organisms on the synthesis of cyanobacterial metabolites were well documented [5][6][7]. It was expected that those factors might stimulate the production of OsPP.…”

Section: E3s Web Of Conferences 47mentioning

confidence: 99%

See 1 more Smart Citation

Effect of medium and light quality on pink pigment production of cyanobacteria Oscillatoria sp. BTCC/A0004

2018

View full text Add to dashboard Cite

Abstract. Cyanobacteria are well known as promising source of valuable chemicals for human usage. Especially, cyanobacteria in tropical area are very wide in diversity and they are potent producers of unique metabolites which exhibit interesting bioactivities. Oscilatoria sp. BTCC/A0004 produce pink pigments extracellularly (OsPP). The effects of various environmental factors on the production of cyanobacteria metabolites were well documented. In this research, the effect of medium and light quality on cell growth and OsPP production were investigated. In case, three different culture media, named No 18, C, and modified C media, in which nutrient compositions are different, and light quality (white, blue, green, pink) were tested. The highest cell growth and OsPP production were obtained in modified C medium.

show abstract

Effect of the Nitrogen Source on Phycobiliprotein Synthesis and Cell Reserves in A Chromatically Adapting Filamentous Cyanobacterium

Cited by 99 publications

References 60 publications

Potentially harmful cyanobacteria in oyster banks of Términos lagoon, southeastern Gulf of Mexico

Potentially harmful cyanobacteria in oyster banks of Términos lagoon, southeastern Gulf of Mexico

Isolation of Cyanophycin-degrading Bacteria, Cloning and Characterization of an Extracellular Cyanophycinase Gene (cphE) from Pseudomonas anguilliseptica Strain BI

Effect of medium and light quality on pink pigment production of cyanobacteria Oscillatoria sp. BTCC/A0004

Contact Info

Product

Resources

About