The saturated absorption technique is an elegant method widely used in atomic and molecular physics for high-resolution spectroscopy, laser frequency standards and metrology purposes. We have recently discovered that a saturated absorption scheme with a dual-frequency laser can lead to a significant sign reversal of the usual Doppler-free dip, yielding a deep enhanced-absorption spike. In this paper, we report detailed experimental investigations of this phenomenon, together with a full indepth theoretical description. It is shown that several physical effects can support or oppose the formation of the high-contrast central spike in the absorption profile. The physical conditions for which all these effects act constructively and result in very bright Doppler-free resonances are revealed. Apart from their theoretical interest, results obtained in this manuscript are of great interest for laser spectroscopy and laser frequency stabilization purposes, with applications in laser cooling, matterwave sensors, atomic clocks or quantum optics. the atomic resonant frequency, the two counter-propagating waves drive different atom groups (of respective axial velocities v + and v -) due to the Doppler shift, which yields the Doppler profile. By contrast, when the frequency detuning is being scanned near the resonance frequency, the two waves act on the same group of atoms with v 0 » . Each wave reduces the absorption experienced by the other. Consequently, one can observe a Doppler-free dip in the absorption profile of one wave or a reduction in total fluorescence of the cell. This dip, with Lorentzian profile and natural linewidth, is known as the Lamb dip, or as saturated absorption resonance (SAR). It can be several orders of magnitude narrower than the Doppler-broadened spectrum in which it sits. A simple two-level scheme (with non-degenerate energy levels) can also exhibit SAR as a single peak or with a more complex shape such as those induced by the recoil effect [17][18][19], high-order spatial harmonics of atomic polarization [20] or peculiarities of the Doppler effect for open atomic transitions [21]. Multilevel atoms can exhibit a complex energy-level structure involving fine and hyperfine splittings and Zeeman (magnetic) sublevels. This complex structure yields complex optical pumping processes, justifying for example the detection of cross-over resonances in saturated absorption spectra or, under appropriate conditions, a sign reversal of saturation resonances [22][23][24][25][26][27].In a recent paper [28], an unexpected deep and narrow reversed spike (strong absorption peak) was observed in a saturated absorption experiment using a dual-frequency laser. The experiment was performed on a Cs vapor cell. The laser carrier frequency was tuned on the Cs D 1 line, with a frequency difference between the two firstorder sidebands equal to the hyperfine splitting (HFS) of the ground state. This phenomenon was applied to improve by about one order of magnitude the fractional frequency stability of a diode laser compared...