Effect of the rooster semen enrichment with oleic acid on the quality of semen during chilled storage

Abstract

Liquid storage of avian spermatozoa is currently being employed in programs utilizing the artificial insemination to optimize the management of genetically superior males. It is mandatory to use efficient semen storage techniques in order to prevent the reduction of the fertilizing ability of stored semen. The present study was designated to evaluate the effect of oleic acid on rooster semen quality stored at 4°C for 48 h. Semen was collected from 10 roosters twice a week. Good quality ejaculates were pooled and after dilution, the semen was enriched with 0 (control), 0.125 (O 0.125), 0.25 (O 0.25), 0.5 (O 0.5), and 1 (O1) millimolar oleate. Forward progressive motility and viability of spermatozoa were evaluated at 0, 24, and 48 h. Moreover, malondialdehyde (MDA) and total antioxidant activity (AOA) levels were measured in seminal plasma and spermatozoa at the mentioned time points. Motility was 80.33 ± 1.45, 80.00 ± 2.08, and 66.00 ± 2.30% at 24 h and 56.33 ± 1.45, 57.33 ± 2.18, and 41.33 ± 2.02% at 48 h in O 0.125, O 0.25, and control, respectively (P < 0.001). Total AOA concentrations of seminal plasma were significantly higher in oleate treated groups than the control at 24 and 48 h (P < 0.03). Moreover, concentrations of AOA in spermatozoa revealed that oleate treated group showed higher AOA values compared to the control group at 24 and 48 h (P < 0.001). MDA concentrations of seminal plasma and spermatozoa were lower in oleate treated groups in comparison with control group at 24 and 48 h (P < 0.05). In conclusion, rooster semen enrichment with low doses of oleate would exert beneficial effects on the quality of semen during cooled storage.

Ameliorative role of trans-ferulic acid on induced oxidative toxicity of rooster semen by β-cyfluthrin during low temperature liquid storage

“…Irreversible damages and changes in the function and fertilization potential of spermatozoa has been reported during cooling process of semen sample ( Yeste, 2016 ). Enrichment of semen extender have been proposed to ameliorate oxidative toxicity during semen storage as liquid or freeze state ( Câmara et al, 2011 ; Eslami et al, 2016 ; Benhenia et al, 2018 ; Ghaniei et al, 2019 ). Results of the current study revealed the higher amounts of MDA in β-cyfluthrin alone exposed group compared to β-cyfluthrin plus 10 and 25 mM trans -FA treated groups.…”

Quercetin attenuates H2O2‐induced toxicity of rooster semen during liquid storage at 4°C

“…Our results showed that rooster semen supplementation with quercetin improved sperm parameters such as forward progressive motility, viability and plasma membrane integrity during hydrogen peroxide‐induced oxidative toxicity. Protective effects of substances with antioxidant properties on rooster sperm motility and viability were demonstrated (Eslami et al., ; Lotfi et al., ; Mirzaei Rad, Eslami, & Ghaniei, ). Moreover, the positive association between progressive motility and fertility following insemination was shown in avian (Fattah et al., ; Lotfi et al., ; Moghbeli et al., ).…”

“…A previous study showed that endogenous antioxidant capacity of semen is not sufficient to conquer oxidative damage during storage (Aurich, Schönherr, Hoppe, & Aurich, ). Semen enrichment with various compounds and antioxidants ameliorate detrimental effects of ROS and lipid peroxidation during chilled storage or after freeze–thawing process in different species, including boar (Funahashi & Sano, ), bull (Munsi, Bhuiyan, Majumder, & Alam, ), ram (Maxwell & Stojanov, ), stallion (Aurich et al., ), rooster (Eslami, Ghaniei, & Mirzaei Rad, ; Lotfi, Mehri, Sharafi, & Masoudi, ; Moghbeli et al., ) and cat (Baran et al., ) semen.…”

Natural Astaxanthin From Haematococcus Pluvialis Enhanced Antioxidant Capacity And Improved Semen Quality In Aging Layer Breeder Roosters Through The Mitogen-Activated Protein Kinase/Nuclear Factor-Erythroid 2-Related Factor 2 (MAPK/Nrf2) Pathway

“…Therefore, in the present study, we aimed to improve the quality of semen by improving the antioxidant capacity of aging roosters using dietary ASTA. Moreover, it has been demonstrated that antioxidant capacity plays an important role in maintaining sperm viability because it can protect against lipid peroxidation in semen [39]. MDA is an important indicator of lipid peroxidation.…”