Effect of the sesterterpene-type metabolites, ophiobolins A and B, on zygomycetes fungi

Krizsán, Krisztina; Bencsik, Ottó; Nyilasi, Ildikó; Galgóczy, László; Vágvölgyi, Csaba; Papp, Tamás

doi:10.1111/j.1574-6968.2010.02138.x

Cited by 22 publications

(12 citation statements)

References 27 publications

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“…Fragmentation of nuclei was detected in resting PK-15 cells after the exposure of the monolayer to 6-epi-ophiobolin K-, ophiobolin K-, and ophiobolin G-containing crude extract from A. calidoustus MH34, which has also been described in relation to ophiobolin A in the L1210 cell line. An apoptosis-like cell death process was also observed in M. circinelloides after 1.6 µg mL −1 ophiobolin A treatment and for ophiobolin O in MCF-7 cells [23][24][25][26]. Ophiobolin A was able to induce paraptosis-like cell death in human glioblastoma multiforme cells by decreasing the big/large conductance Ca 2+ -activated K + channel activity [8,9].…”

Section: Discussionmentioning

confidence: 75%

Screening Mold Colonies by Using Two Toxicity Assays Revealed Indoor Strains of Aspergillus calidoustus Producing Ophiobolins G and K

Salo

Marik

Bencsik

et al. 2019

Toxins

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The occurrence and toxin production of the opportunistic pathogen Aspergillus calidoustus in Finnish buildings is not well documented in the literature. We tracked and identified four A. calidoustus colonies cultivated from indoor settled dusts and revealed the biological activities of crude biomass extracts. The toxic substances were identified as 6-epi-ophiobolin K, ophiobolin K, and ophiobolin G by high-performance liquid chromatography–mass spectrometry (HPLC-MS) based on chromatographic and mass spectrometry data (MS and MS/MS) on the crude extract of A. calidoustus strain MH34. A total of 29 fungal colonies collected from settled dust in an office room reported for indoor-air-related illnesses were screened for toxins that inhibited boar sperm motility in the BSMI (boar sperm motility inhibiting) assay and cell proliferation in the ICP (inhibition of cell proliferation) assays with PK-15 cells. Out of the 27 colonies tested as toxic, 12 colonies exhibiting conidiophores representative of the genera Chaetomium, Penicillium, and Paecilomyces were excluded from the study, while 13 colonies exhibited Aspergillus-like conidiophores. Biomass suspensions of these colonies were divided into two categories: Category 1 colonies (n = 4), toxic in the BSMI assay and the ICP assays, emitted blue fluorescence and grew at 37 °C; Category 2 colonies (n = 9), only toxic in the ICP assay, emitted orange fluorescence and exhibited limited or no growth at 37 °C. Colonies in Category 1 were pure-cultured, and the strains were named as MH4, MH21, MH34, MH36. Strain MH34 was identified as A. calidoustus by the internal transcribed spacer (ITS) sequences. Ethanol-soluble dry substances extracted from the biomass of the pure cultures exhibited a toxicological profile in the BSMI assay, SMID (sperm membrane integrity damage) assay, and ICP assay similar to that exhibited by pure ophiobolin A. Overall, the viable conidia of A. calidoustus in indoor settled dusts deserve attention when potentially hazardous mold species are monitored.

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Section: Discussionmentioning

confidence: 75%

Screening Mold Colonies by Using Two Toxicity Assays Revealed Indoor Strains of Aspergillus calidoustus Producing Ophiobolins G and K

Salo

Marik

Bencsik

et al. 2019

Toxins

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“…In addition to T-toxin for example, C. heterostrophus also produces ophiobolins that are sesquiterpene phytotoxins ( Fig. Ophiobolins are also active against certain Zygomycetes as they inhibit sporangiospore germination of Mucor circinelloides and in addition cause morphological defects (Krizsan et al, 2010). A mechanism for their antimicrobial and antitumor activity was proposed based on competition for binding to the membrane ivermectin receptor, which modulates an invertebrate specific glutamate-gated chloride channel (Tsipouras et al, 1996).…”

Section: Non-hsts Produced By Plant Pathogenic Dothideomycetesmentioning

confidence: 99%

Phytotoxic secondary metabolites and peptides produced by plant pathogenicDothideomycetefungi

Stergiopoulos¹,

Collemare²,

Mehrabi³

et al. 2013

FEMS Microbiol Rev

169

103

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Many necrotrophic plant pathogenic fungi belonging to the class of Dothideomycetes produce phytotoxic metabolites and peptides that are usually required for pathogenicity. Phytotoxins that affect a broad range of plant species are known as non-host-specific toxins (non-HSTs), whereas HSTs affect only a particular plant species or more often genotypes of that species. For pathogens producing HSTs, pathogenicity and host specificity are largely defined by the ability to produce the toxin, while plant susceptibility is dependent on the presence of the toxin target. Non-HSTs are not the main determinants of pathogenicity but contribute to virulence of the producing pathogen. Dothideomycetes are remarkable for the production of toxins, particularly HSTs because they are the only fungal species known so far to produce them. The synthesis, regulation, and mechanisms of action of the most important HSTs and non-HSTs will be discussed. Studies on the mode of action of HSTs have highlighted the induction of programed cell death (PCD) as an important mechanism. We discuss HST-induced PCD and the plant hypersensitive response upon recognition of avirulence factors that share common pathways. In this respect, although nucleotide-binding-site-leucine-rich repeat types of resistance proteins mediate resistance against biotrophs, they can also contribute to susceptibility toward necrotrophs.

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“…These six subclasses of sesterterpenoids have been accounted for to display noteworthy cytotoxicities against tumor cells [28]. Ophiobolins have pulled in across the board consideration due to their phytotoxic, antimicrobial, nematocidal and cytotoxic bioactivities [29,30] (Figure 14-16).…”

Section: Diterpenes and Sesterterpenesmentioning

confidence: 99%

Chemistry of Secondary Metabolites

Mohiuddin¹

2019

ACT

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Medicinal plants, are known to deliver a wide scope of Plant Secondary Metabolites (PSMs) connected as bug sprays, medications, colors and poisons in farming, prescription, industry and biofighting in addition to bio-fear mongering, separately. Be that as it may, creation of PSMs is for the most part in little amounts, so we have to discover novel approaches to increment both amount and nature of them. Luckily, biotechnology proposes a few choices through which secondary metabolism in plants can be built in imaginative approaches to: 1) over-produce the valuable metabolites, 2) down-produce the poisonous metabolites, 3) produce the new metabolites. Secondary metabolites are extensively characterized as natural products integrated by a life form that is not basic to help development and life. The plant kingdom fabricates more than 200,000 unmistakable chemical compounds, a large portion of which emerge from particular metabolism. While these compounds assume critical jobs in interspecies challenge and safeguard, many plant natural products have been abused for use as prescriptions, scents, flavors, supplements, repellants, and colorants. In spite of this immense chemical decent variety, numerous secondary metabolites are available at low focuses in plant a, taking out yield based assembling as methods for achieving these imperative products. The basic and stereo chemical unpredictability of particular metabolites frustrates most endeavors to get to these compounds utilizing chemical blend. Albeit local plants can be built in amass target pathway metabolites. This Update gives a concise outline of designing plant secondary metabolism in microbial frameworks. We quickly diagram biosynthetic pathways intervening arrangement of the real classes of natural products with an accentuation on high-esteem terpenoids, alkaloids, phenylpropanoids, and polyketides. We additionally feature basic topics, techniques, and provoke hidden endeavors to reproduce and build these pathways in microbial hosts. We center mostly around again biosynthetic methodologies in which plant specific metabolites are combined straightforwardly from sugar feed stocks instead of enhanced forerunners or intermediates.

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Effect of the sesterterpene-type metabolites, ophiobolins A and B, on zygomycetes fungi

Cited by 22 publications

References 27 publications

Screening Mold Colonies by Using Two Toxicity Assays Revealed Indoor Strains of Aspergillus calidoustus Producing Ophiobolins G and K

Screening Mold Colonies by Using Two Toxicity Assays Revealed Indoor Strains of Aspergillus calidoustus Producing Ophiobolins G and K

Phytotoxic secondary metabolites and peptides produced by plant pathogenicDothideomycetefungi

Chemistry of Secondary Metabolites

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