Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC

Subirats, Xavier; Casanovas, Laura Vall llosera; Redón, Lídia; Rosés, Martı́

doi:10.1016/j.sampre.2023.100063

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…Such an outcome may be attributed to the destabilization of the water layer on the stationary-phase surface in the presence of protic MeOH molecules. The protic nature of MeOH promotes hydrogen bonding among the solvent molecules, the ONs, and the amide stationary phase, 43 thus suppressing molecular interactions that drive ON diastereomer separation. Another potential explanation for such a MeOH-driven diastereomer separation suppression is that the presence of MeOH may disrupt the ON HOS in solution.…”

Section: Resultsmentioning

confidence: 99%

Unravelling the Link between Oligonucleotide Structure and Diastereomer Separation in Hydrophilic Interaction Chromatography

Lardeux,

Stavenhagen,

Paris

et al. 2024

Anal. Chem.

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Section: Resultsmentioning

confidence: 99%

Unravelling the Link between Oligonucleotide Structure and Diastereomer Separation in Hydrophilic Interaction Chromatography

Lardeux,

Stavenhagen,

Paris

et al. 2024

Anal. Chem.

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“…The analysis of acids and bases requires the use of buffered mobile phases, which is not straightforward in HILIC eluents containing a high proportion of organic solvents [15]. Buffers are needed to ensure reproducibility of the results as well as to improve peak shape [31,32].…”

Section: Methodsmentioning

confidence: 99%

“…Interactions between the column surface and analytes in the mobile phase are critical for the separation of components in HILIC, suggesting that the separation mechanism differs for each pair of mobile phase and column. Additionally, these interactions depend on the type and ionic state of analytes [15,16].…”

Section: Introductionmentioning

confidence: 99%

Retention mechanisms of amitriptyline and its impurities on amide, amino, diol, and silica columns in hydrophilic interaction liquid chromatography

Knežević Ratković,

Kukobat,

Kasagić‐Vujanović

2024

J of Separation Science

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Hydrophilic interaction liquid chromatography (HILIC) has been widely applied to challenging analysis in biomedical and pharmaceutical fields, bridging the gap between normal‐phase high‐performance liquid chromatography and reversed‐phase high‐performance liquid chromatography (RP‐HPLC). This paper comprehensively explores the retention mechanisms of amitriptyline and its impurities A, B, C, D, F, and G on amide, amino, diol, and silica columns. Dual HILIC/RP‐HPLC retention mechanisms were developed, and transitional points between HILIC and RP‐HPLC mechanisms were calculated on amide, diol, and silica columns. Adsorption and partition contributions to overall retention mechanisms were evaluated using Python software in HILIC and RP‐HPLC regions. The cation exchange mechanism dominates overall retention for ionized analytes in the silica column (R2 > 0.995), whereas the retention of ionized analytes increases with pH. Impacts of acetonitrile content, buffer ionic strength, and pH, along with their interactions on the retention of ionized analytes in the silica column, were determined using the chemometric approach. Acetonitrile content showed the most significant impact on the retention mechanisms. These findings highlight that a detailed investigation into retention mechanisms provides notable insights into factors influencing analyte retention and separation, promising valuable guidance for future analysis.

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“…Additional solvents such as alcohols and acetone are also utilized. Besides the solvent composition, the retention process is influenced by factors such as the type and quantity of salt, along with pH and temperature [7].…”

Section: Introductionmentioning

confidence: 99%

Exploring separation mechanisms and lipophilicity in hydrophilic interaction chromatography conditions by thin‐layer chromatography of anesthetics and adjuvant drugs as polar model compounds

Radoičić,

Šegan,

Milojković‐Opsenica

2024

J of Separation Science

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The chromatographic behavior of the selected compounds was studied under conditions of hydrophilic interaction liquid chromatography (HILIC). The effect of mobile phase composition on the retention in different chromatographic systems was systematically examined using high‐performance thin‐layer chromatography. The sorbents of different polarity and adsorption characteristics were selected and mixtures of water and organic solvents of various compositions, from pure water to pure organic solvent were used as mobile phases. Increasing the amount of water in the mobile phase leads to a conversion of the separation mechanism, and the retention curves have a characteristic “U” shape. The conversion between the adsorption and partition mechanisms is most likely continuous and depends on the chemical nature of separated substances, the stationary phase as well as on organic component of the mobile phase. Silica gel can be considered the most suitable stationary phase for the systematic investigation of the chromatographic behavior of the test compounds, whereas acetonitrile was the most suitable solvent. The obtained results contribute to the understanding of the dominant separation mechanism, the type, and the intensity of the interactions between separated substances with both stationary and mobile phases. Besides, the lipophilicity parameters obtained under HILIC conditions were evaluated and correlated with the calculated values.

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Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC

Cited by 6 publications

References 12 publications

Unravelling the Link between Oligonucleotide Structure and Diastereomer Separation in Hydrophilic Interaction Chromatography

Unravelling the Link between Oligonucleotide Structure and Diastereomer Separation in Hydrophilic Interaction Chromatography

Retention mechanisms of amitriptyline and its impurities on amide, amino, diol, and silica columns in hydrophilic interaction liquid chromatography

Exploring separation mechanisms and lipophilicity in hydrophilic interaction chromatography conditions by thin‐layer chromatography of anesthetics and adjuvant drugs as polar model compounds

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