Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content

Kurze, Elisabeth; Kock, Vanessa; Scalzo, Roberto Lo; Olbricht, Klaus; Schwab, Wilfried

doi:10.3390/nu10070857

Cited by 18 publications

(19 citation statements)

References 38 publications

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“…From these results, we concluded that fruit color is not indicative of allergenicity related to Fra a 1.01. This finding was consistent with those of two previous studies on European varieties harvested in spring (Franz-Oberdorf et al, 2017;Kurze et al, 2018). One of the major reasons for no interaction between Fra a 1 expression and fruit color is the genetic variation in the flavonoid biosynthesis pathway in each white cultivar, that is, different mutations in an enzyme like phenylalanine ammonia-lyase (PAL) and/or dihydrokaempferol 4reductase (DFR), a transcription factor like MYB, or a binding protein to metabolic intermediates like Fra a 1.…”

Section: Rough Screening Of Cultivarssupporting

confidence: 94%

“…The pattern of change was either up-or down-regulation in each genotype. In Germany, there was no significant annual change in Fra a 1 protein levels among fresh fruits of a red cultivar (Kurze et al, 2018). To our knowledge, this is the first report that Fra a 1 concentrations in strawberries differ during the harvest season and among months.…”

Section: Seasonal Variation In Fra a 1 Transcript Levels And Fra A 1 mentioning

confidence: 58%

“…Strawberry (Fragaria × ananassa) is a popular fruit worldwide. In addition to its versatility as a fresh or processed food, it has become a popular food for human health because it contains bioavailable components such as phenolic compounds (Afrin et al, 2016;Giampieri et al, 2014;Kurze et al, 2018). However, strawberries also contain allergens that can cause oral allergic syndrome (OAS) in some people.…”

Section: Introductionmentioning

confidence: 99%

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Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

et al. 2019

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Strawberry (Fragaria × ananassa) contains a major allergen, Fra a 1, which causes oral allergic syndrome. Fra a 1 is a PR-10 homolog that is regulated by environmental conditions. The allergenicity of fruit caused by Fra a 1 may depend on the genotype or growing conditions. We analyzed the Fra a 1.01 transcript levels and Fra a 1.01 protein levels in strawberry fruits of several genotypes across all seasons. In the preliminary rough screening, we selected the line WH1 bearing white fruit and the red-fruited cultivar 'Akihime'. Under the same environmental conditions, there was no significant difference in Fra a 1.01 levels between the two cultivars over several months, suggesting that receptacle color was not indicative of allergenicity caused by Fra a 1.01. Fruits cultivated under the same environmental conditions should be used for comparisons of the allergenicity among genotypes. Both 'Akihime' and WH1 accumulated significantly higher levels of Fra a 1.01 protein in winter than in spring. We investigated the effects of irradiation and low temperature as environmental factors controlling the accumulation of Fra a 1.01 in winter. A shading treatment on fruit did not significantly affect Fra a 1.01 protein accumulation in strawberry fruits. Regarding variations over time, the Fra a 1.01 protein content was higher in fruits harvested at midnight in January than in those harvested at other times and in other months. These findings suggested that the Fra a 1.01 protein accumulates in response to environmental factors such as cold stress.

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Section: Rough Screening Of Cultivarssupporting

confidence: 94%

Section: Seasonal Variation In Fra a 1 Transcript Levels And Fra A 1 mentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

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Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

et al. 2019

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“…The prevalence of food allergies ranges from 2-10% in the US depending on the state [ 9 ]. Although less common, allergies to certain fruits such as strawberries [ 10 ] drive the population to avoid them. Many fruits, for example peaches, apples, and apricots, lead to irritation in the digestive tract due to the high fructose content, which is a concern for people suffering from irritable bowel syndrome (IBS) [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Dietary Supplementation of Whole Foods and Polyphenols on Atherosclerosis

et al. 2020

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The purpose of this review is to highlight current research on the benefits of supplementation with foods with a diverse polyphenol composition, including fruits, vegetables, nuts, grains, oils, spices, and teas in blunting atherosclerosis. We searched PubMed for publications utilizing whole food or polyphenols prepared from whole foods in Apolipoprotein E (ApoE) or Low-Density Lipoprotein Receptor (LDLR) knockout mice, and identified 73 studies in which plaque was measured. The majority of the studies reported a reduction in plaque. Nine interventions showed no effect, while three using Agaricus blazei mushroom, HYJA-ri-4 rice variety, and safrole-2’, 3’-oxide (SFO) increased plaque. The mechanisms by which atherosclerosis was reduced include improved lipid profile, antioxidant status, and cholesterol clearance, and reduced inflammation. Importantly, not all dietary interventions that reduce plaque showed an improvement in lipid profile. Additionally, we found that, out of 73 studies, only 9 used female mice and only 6 compared both sexes. Only one study compared the two models (LDLR vs. ApoE), showing that the treatment worked in one but not the other. Not all supplementations work in both male and female animals, suggesting that increasing the variety of foods with different polyphenol compositions may be more effective in mitigating atherosclerosis.

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“…Several external stimuli induce Fra a 1 expression, including UV-C radiation, chitosan treatments, and achene removal (Ishibashi et al, 2017;Petriccione et al, 2017;Severo et al, 2015). Differences in Fra a 1 expression levels in varieties have been reported in some cultivation areas (Alm et al, 2007;Franz-Oberdorf et al, 2016); however, differences, as assessed by Fra a 1 protein accumulation levels, have not been documented in various production areas, including Japan (Franz-Oberdorf et al, 2017;Ishibashi et al, 2019b;Kurze et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Response of the Major Allergen Fra a 1.01 in Strawberry to Cold

et al. 2020

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Fra a 1 is a strawberry allergen that causes oral allergic syndrome. Fra a 1.01 is a major isoform that accumulates abundantly in fruits during the winter season. Here, we tested the hypothesis that Fra a 1.01 responds to environmental factors, such as cold stress. We analyzed transcriptional and translational levels of Fra a 1.01 in strawberry calli and organs under various cold conditions. First, we incubated strawberry (Fragaria × ananassa 'Akihime') calli and post-harvested fruits at low temperatures from several hours to days. Fra a 1.01 did not show significant differences in either gene expression or protein accumulation levels, suggesting that short-term cold treatments did not affect Fra a 1.01 expression. Second, we exposed whole plants to low temperature conditions for ~28 days. Under conditions below 10°C, Fra a 1.01 transcripts were induced gradually throughout the cold treatment (crown and root), or from 2 days to the last day (leaf and fruit). The Fra a 1.01 protein remarkably accumulated in crowns and slightly in fruits after 28 days. Finally, the promoter region of Fra a 1.01e was analyzed to detect tissue-specific expression. The cloned and sequenced promoter included several cis-acting regulatory elements related to cold response. When the Fra a 1.01 promoter region was heterologously expressed in Arabidopsis, the promoter activities, as assessed by GUS staining, were observed mainly around the shoot apices and in roots. Thus, Fra a 1.01 was considered to be expressed in crowns and roots, and was additively induced by cold stress. These organ-specific expressions could be important in elucidating the mechanisms responsible for Fra a 1.01 protein's accumulation in fruits during the winter season.

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Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content

Cited by 18 publications

References 38 publications

Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

The Impact of Dietary Supplementation of Whole Foods and Polyphenols on Atherosclerosis

Response of the Major Allergen Fra a 1.01 in Strawberry to Cold

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