Effect of the TLR2/MyD88/NF-κB axis on corneal allograft rejection after penetrating keratoplasty

Wu, Wei; Yu, Shengyou; Feng, Songfu; Yang, Jize; Lu, Xiaoyan

doi:10.3109/10799893.2015.1016578

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…BM-MSCs are pluripotent stem cells with low immunogenicity, which play a role in immune regulation and stem cell homing and promote anti-inflammatory and tissue repair processes (9)(10)(11)(12)(13). It has been reported that the exosomes derived from BM-MSCs can promote the repair of corneal and conjunctival damage caused by chemical injuries and diabetic corneal epithelia; however, the underlying mechanism has not been elucidated (14)(15)(16)(17). In this study, the exosomes derived from BM-MSCs were labeled with PKH-26.…”

Section: Discussionmentioning

confidence: 99%

Effect of PKH-26-labeled exosomes derived from bone marrow mesenchymal stem cells on corneal epithelium regeneration in diabetic mice

Wu¹,

Zhou²,

Zhu³

et al. 2023

Ann Transl Med

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Background: It is known that bone marrow mesenchymal stem cells (BM-MSCs) could speed up the regeneration of diabetic corneal epithelium. To investigate the effect of exosomes derived from mouse BM-MSCs on corneal epithelium regeneration in diabetic mice.Methods: Diabetic mouse models were established using streptozotocin (STZ), and their central corneal epithelium was scratched under a microscope. The diabetic mice were randomly divided into three groups: the control group was injected with subconjunctival phosphate buffer saline; the exosomes group was treated with a subconjunctival injection of exosomes derived from BM-MSCs; and the BM-MSCs group was treated with a subconjunctival injection of BM-MSCs. The corneal epithelium repair rates in the three groups were compared, and the distribution of the exosomes derived from BM-MSCs labeled with PKH-26 was observed by immunofluorescence. Hematoxylin-eosin staining of the corneal tissue was observed 72 h after the treatments in the three groups.Results: The diabetic mice were successfully established by a blood glucose level >16.7 mmol/L after 8 weeks. The corneal epithelium healing rates in experimental groups 1 and 2 were significantly higher than those of the control group at 24, 48, and 72 h (P<0.05). However, there was no significant difference in the corneal epithelial healing rate between experimental groups 1 and 2 (P>0.05). The exosomes derived from BM-MSCs were found in the superficial corneal stroma in experimental groups 1 and 2, with the majority of the exosomes distributed in the limbal epithelium at the edge of the injury area. The proliferation of corneal epithelial cells in experimental groups 1 and 2 was more obvious than that in the control group. Conclusions:The exosomes derived from BM-MSCs labeled with PKH-26 significantly promoted the repair of corneal epithelial injury in diabetic mice. These exosomes might be a substitute for BM-MSCs in the repair of diabetic keratopathy, suggesting a new idea for the repair of diabetic keratopathy with "cell-free" stem cell therapy, which will require a clinical study.

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