Effect of the tocotrienol-rich fraction on the lifespan and oxidative biomarkers in Caenorhabditis elegans under oxidative stress

Abstract

OBJECTIVE:This study was performed to determine the effect of the tocotrienol-rich fraction on the lifespan and oxidative status of C. elegans under oxidative stress.METHOD:Lifespan was determined by counting the number of surviving nematodes daily under a dissecting microscope after treatment with hydrogen peroxide and the tocotrienol-rich fraction. The evaluated oxidative markers included lipofuscin, which was measured using a fluorescent microscope, and protein carbonyl and 8-hydroxy-2′-deoxyguanosine, which were measured using commercially available kits.RESULTS:Hydrogen peroxide-induced oxidative stress significantly decreased the mean lifespan of C. elegans, which was restored to that of the control by the tocotrienol-rich fraction when administered before or both before and after the hydrogen peroxide. The accumulation of the age marker lipofuscin, which increased with hydrogen peroxide exposure, was decreased with upon treatment with the tocotrienol-rich fraction (p<0.05). The level of 8-hydroxy-2′-deoxyguanosine significantly increased in the hydrogen peroxide-induced group relative to the control. Treatment with the tocotrienol-rich fraction before or after hydrogen peroxide induction also increased the level of 8-hydroxy-2′-deoxyguanosine relative to the control. However, neither hydrogen peroxide nor the tocotrienol-rich fraction treatment affected the protein carbonyl content of the nematodes.CONCLUSION:The tocotrienol-rich fraction restored the lifespan of oxidative stress-induced C. elegans and reduced the accumulation of lipofuscin but did not affect protein damage. In addition, DNA oxidation was increased.

Achiote (Bixa orellana) Lipophilic Extract, Bixin, and δ-tocotrienol Effects on Lifespan and Stress Resistance in Caenorhabditis elegans

“…The results about both major components in AE point to dT3 as the main compound responsible for the protective effect; however, this protection can be enhanced by an accumulative effect when bixin is present, which suggests an independent mechanism for each compound. The protective effects of α-tocopherol: tocotrienol mixtures have previously been studied by Adachi and Ishii [26] and by Aan et al [27]. These authors reported an increase in median life as well as resistance to UV and oxidative (H 2 O 2 ) stress, which is in line with our results.…”

Coffee Silverskin Extract Protects against Accelerated Aging Caused by Oxidative Agents

“…It is well known that UV radiation is the main cause of photoaging and induces cell and tissue damage as the production of ROS, which leads to DNA damage [36]. In this sense, CSE could be reducing the oxidative stress accumulation and, therefore, the DNA damage, as previously demonstrated with other antioxidant compounds, such as tocotrienol [37]. Although previous reports have been made about the functional properties of coffee in C. elegans [38,39], we report for the first time the potential of a natural extract from coffee silverskin by-product for UV radiation protection, which could be very interesting for dermo- and nutria-cosmetic companies developing new products targeting photoaging.…”

Rhizobium induces DNA damage in Caenorhabditis elegans intestinal cells

“…But, if DNA damage is localized to only intestinal cells (less than 1% of the DNA content of a fertile hermaphrodite), we would not expect to see a dramatic change in 8-OHdG as measured for the entire DNA content of a C. elegans. In published experiments, treatment of C. elegans with hydrogen peroxide, a known agent promoting formation of 8-OHdG (37), resulted only in a modest increase in , from 20 to 25 pg per microgram of chromosomal DNA, despite high dosage of hydrogen peroxide and lengthy treatment (38). Thus, our results should not be interpreted as an absence of a change in 8-OHdG as this assay might not be sensitive enough to reveal the change in chromosomal DNA of intestinal cells.…”