Effect of THP-1 Cells on the Formation of Vascular Tubes by Endothelial EA.hy926 Cells in the Presence of Placenta Secretory Products

Lvova, T. Yu.; Belyakova, K. L.; Сельков, С. А.; Sokolov, D. N.

doi:10.1007/s10517-017-3657-6

Cited by 2 publications

(3 citation statements)

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“…The significance of differences between groups: *, p < 0.05; **, p < 0.01; ***, p < 0.001 (differs from the spontaneous level); #, p < 0.05; ##, p < 0.01; ###, p < 0.001. [16,25,26,44]. В связи с этим можно предположить, что увеличение длины сосудов может быть вызвано продукцией этих цитокинов клетками линии THP-1.…”

Section: Discussionunclassified

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Influence of VEGF deprivation upon vascular formation by endothelium in the presence of macrophages

et al. 2020

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Development of angiogenesis depends on the functional state of endothelial cells, as well as on the balanced secretion of cytokines, growth factors and chemokines by endothelial cells and cells of microenvironment. Macrophages represent an essential component of the microenvironment and take part in the formation of blood vessels both due to the production of cytokines and due to contact interactions with endothelial cells. VEGF is among the most important cytokines that control angiogenesis at all its stages. Currently, the role of VEGF in the intercellular interactions of endothelial cells and macrophages is not well described. The aim of our study was to investigate the effect of VEGF deprivation using monoclonal antibodies on angiogenesis under conditions of co-cultivation of endothelium and macrophages. Materials and methods: monoclonal antibodies to VEGF-A were used for VEGF deprivation in monoculture of endothelial cells and in co-culture of endothelial cells with macrophages. The IL-1β, IL-6 and TNFα cytokines were used as inducers. When VEGF-A was removed from the medium, endothelial cells show plasticity and form longer vessels, they modify the expression of VEGF receptors. Macrophages regulate endothelial cell activity through the secretion of cytokines, including VEGF, and through contact interactions with endothelial cells. THP-1 cells increase the sensitivity of endothelial cells to VEGF by stimulating the VEGFR1 and VEGFR3 expression, this effect is VEGF-A-independent. The IL-1β, IL-6, TNFa cytokines independently stimulate non-branching angiogenesis, increasing the length of the vessels. At the same time, IL-ip increases the VEGFR1 expression on the surface of endothelial cells. In contrast, IL-6 and TNFα decrease it, thereby regulating the sensitivity of endothelial cells to VEGF. The effects of these cytokines are not dependent on VEGF-A. The IL-1β, IL-6, TNFα cytokines promote acquisition of anti-angiogenic properties by THP-1 cells that is independent on VEGF-A, as well as on expression of its receptors by endothelial cells. Thus, VEGF is an important, but not the sole factor controlling angiogenesis. Under conditions of VEGF-A deficiency, either endothelial cells or microenvironment cells are able to compensate for its functional load due to the production of other growth factors.

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“…НИИОЧБ ФМБА России, Санкт-Петербург, Россия), 4 нг/ мл IL-6 (RD, США). Приведенные концентрации были выбраны на основании данных, полученных ранее [24,26]…”

Section: цитокины и препаратыunclassified

Influence of VEGF deprivation upon vascular formation by endothelium in the presence of macrophages

et al. 2020

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“…In each well of the plate, photographs of five random visual fields were taken by the AxioObserver Z1 microscope (Carl Zeiss, Oberkochen, Germany) at a 100× magnification (phase contrast). All angiogenesis experiments have been described and published previously [ 57 , 63 , 64 , 65 ]. Thus, as a result of the experiments, 275 micrographs were obtained and selected for further analysis using a neural network.…”

Section: Materials and Methodsmentioning

confidence: 99%

Deep Semantic Segmentation of Angiogenesis Images

Ibragimov

Сенотрусова

Маркова

et al. 2023

IJMS

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Angiogenesis is the development of new blood vessels from pre-existing ones. It is a complex multifaceted process that is essential for the adequate functioning of human organisms. The investigation of angiogenesis is conducted using various methods. One of the most popular and most serviceable of these methods in vitro is the short-term culture of endothelial cells on Matrigel. However, a significant disadvantage of this method is the manual analysis of a large number of microphotographs. In this regard, it is necessary to develop a technique for automating the annotation of images of capillary-like structures. Despite the increasing use of deep learning in biomedical image analysis, as far as we know, there still has not been a study on the application of this method to angiogenesis images. To the best of our knowledge, this article demonstrates the first tool based on a convolutional Unet++ encoder–decoder architecture for the semantic segmentation of in vitro angiogenesis simulation images followed by the resulting mask postprocessing for data analysis by experts. The first annotated dataset in this field, AngioCells, is also being made publicly available. To create this dataset, participants were recruited into a markup group, an annotation protocol was developed, and an interparticipant agreement study was carried out.

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Effect of THP-1 Cells on the Formation of Vascular Tubes by Endothelial EA.hy926 Cells in the Presence of Placenta Secretory Products

Cited by 2 publications

References 38 publications

Influence of VEGF deprivation upon vascular formation by endothelium in the presence of macrophages

Influence of VEGF deprivation upon vascular formation by endothelium in the presence of macrophages

Deep Semantic Segmentation of Angiogenesis Images

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