Effect of three feeding levels on the pathogenesis and establishment of Haemonchus contortus in parasite-naïve Pelibuey hair sheep lambs during their first infection

Can-Celis, Alhely; Torres-Acosta, J.F.J.; Mancilla-Montelongo, M.G.; González-Pech, Pedro Geraldo; Ramos-Bruno, E.; Sandoval-Castro, Carlos; Vargas-Magaña, J.J.; Bojórquez-Encalada, F.; Cruz-Tamayo, Alvar A.; Canché-Pool, E.; Arellano, María Eugenia López; Galaz-Ávalos, R.M.; Loyola‐Vargas, Víctor M.; Méndez-Ortíz, F.A.

doi:10.1016/j.vetpar.2022.109811

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…The main approach for controlling the GIN including H. contortus is through chemical drugs and their irrational use has trigged an anthelmintic resistance problem worldwide ( Herrera-Manzanilla et al, 2017 ; Baudinette et al, 2022 ). In this sense, several research works using the integral management for control of GIN like grazing rotation, selection of animals resistant to GIN ( Colvin et al ., 2021 ), use of diets rich in protein and energy ( Can-Celis et al, 2022 ), biological control using nematophagous fungi ( Mendoza-de Gives et al, 2022 ) and plant extracts with high content of secondary metabolites have been proposed ( Jayawardene et al, 2021 ). A number of in vitro studies with extracts of several family plants have demonstrated an important anthelmintic effect on GIN including to H. contortus ( Alowanou et al, 2019 ; Davuluri et al, 2020 ; López-Rodríguez et al, 2022 ).…”

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confidence: 99%

Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching

Becerril-Gil,

Estrada-Flores,

González-Cortazar

et al. 2024

Rev. Bras. Parasitol. Vet.

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The aim of this study was to assess the in vitro ovicidal activity of an ethyl acetate extract from Arceuthobium vaginatum (EtOAc-E) and their subfractions (AvR5-AvR14) against Haemonchus contortus using the egg hatching inhibition (EHI) test. The EtOAc-E and subfractions were tested at 0.12-2.00 and at 0.015-2.0 mg/mL, respectively. Distilled water and methanol (2%) were used as negative controls and Thiabendazole (0.10 mg/mL) as a positive control. Treatments with a dependent effect on concentration were subjected to regression analysis to determine the effective concentrations (EC50 and EC90). The major secondary compounds present in the extract and subfractions were identified by high performance liquid chromatography (HPLC). The EtOAc-E and AvR9 exhibited the best ovicidal effect recording 97.5 and 100% of EHI at 0.25 mg/mL, respectively. The EtOAc-E and AvR9 displayed an EC50= 0.12 and 0.08 mg/mL, respectively. The HPLC analysis in the EtOAc-E and bioactive fractions indicated the presence of a polyphenol, glycosylated flavanones, quercetin glucoside, cinnamates, coumarin, cinnamic acid derivative, ferulic acid, coumarate, naringenin, protocatechuic acid and naringin. Results demonstrated that A. vaginatum extract and fraction is able to inhibit the egg hatch process of H. contortus and could be a viable option for the control of small ruminant haemonchosis.

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