Effect of time delay and storage temperature on blood gas and acid–base values of bovine venous blood

Gökçe, Gürbüz; Çitil, Mehmet; Güneş, Vehbi; Atalan, Gültekin

doi:10.1016/j.rvsc.2003.08.009

Cited by 26 publications

(45 citation statements)

References 10 publications

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“…It is even more diffused the idea to realize a laboratorial procedure in the shortest time interval possible after the sample collection. However, in numerous practical daily situations an immediate analysis of the collected blood is not feasible, and thus storage of the sample is necessary 2,3 . In this case, adequate storage implies that the examing results and consequently the analysis, diagnostic and the prescribed treatment must not be influenced by the storage time indicated 3 .…”

Section: Introductionmentioning

confidence: 99%

“…The inadequate handling and usage of material is one of the main causes for errors in blood gas analysis 2,3 , errors that can lead to false results 3 . The possibility of blood samples maintenance at low temperatures is an object of interest in some scientific researches which make use of bovine 2,4,5,6 , equine 5,7,8 , canine 9 and porcine 10 species.…”

Section: Introductionmentioning

confidence: 99%

“…The possibility of blood samples maintenance at low temperatures is an object of interest in some scientific researches which make use of bovine 2,4,5,6 , equine 5,7,8 , canine 9 and porcine 10 species. However, there is a lack of consensus in literature regarding the maximum amount of time blood samples can be stored for blood gas analysis at low temperatures.…”

Section: Introductionmentioning

confidence: 99%

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Effects of blood storage on ice in biochemical and arterial blood gas analysis of rats

et al. 2008

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Purpose:To investigate the effects of blood storage in biochemical and arterial blood gas analysis of Wistar rats. Methods: Ten adult male rats with weights between 300-350 g were used. The catheterization of the internal carotid artery were performed, followed by withdrawal of 3 ml of blood using 3 separate syringes each containing 1 ml. The syringes were fulfilled one after another and numerated according to the order of collection. Then, the following groups were devised: GT1 -samples that were firstly analyzed in 20 minutes and the second exam 65 minutes after the collection; GT2 -samples that were firstly analyzed in 35 minutes and the second exam 80 minutes after the collection; GT3 -samples that were firstly analyzed in 50 minutes and the second exam 95 minutes after the collection. The samples were stored in a container with a temperature between 0 Cº and 4 Cº. Upon analysis the values of pH, PaCO 2 , PaO 2 , HCO 3 -, SatO 2 , SBE, Na + and K + concentrations and glucose were compared. Results: No statistically relevant difference (p<0.05) between the GT1, GT2 or GT3, when measuring pH, PaCO 2 , PaO 2 , HCO 3 -, SatO 2 , SBE or glucose, were found. However, Na + concentration decreased and the K + concentration increased (p<0.05) when comparing the first analysis (20 minutes) with the last one (95 minutes). Conclusion:The blood storage on ice does not interfere in the results of arterial blood gas analysis for the period of at least 95 minutes, except for the Na + e K + levels, which are practicable until 80 minutes after storage. Key words: Blood Preservation. Blood Gas Analysis. Rats. RESUMO Objetivo:Analisar o efeito da estocagem sanguínea na bioquímica e na gasometria do sangue arterial de ratos Wistar. Métodos: Foram utilizados 10 ratos adultos, machos, com peso compreendido entre 300 a 350 gramas. Cada animal foi submetido à cateterização da artéria carótida seguida de heparinização prévia do animal. Foram colhidos 3 ml de sangue total do rato, separados em três seringas contendo 1 ml cada. As seringas foram preenchidas uma após a outra e enumeradas respectivamente à ordem de coleta. De acordo com esta numeração as amostras foram distribuídas nos seguintes grupos: GT1 -amostras que tiveram primeira análise em 20 minutos e a segunda análise 65 minutos após a coleta; GT2 -amostras que tiveram primeira análise em 35 minutos e segunda análise 80 minutos após a coleta; GT3 -amostras que tiveram primeira análise em 50 minutos e 95 minutos após a coleta. As amostras foram estocadas em material isolante térmico, com temperatura entre 0 Cº e 4 Cº.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of blood storage on ice in biochemical and arterial blood gas analysis of rats

et al. 2008

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“…However, there was a markedly increased lactic acid concentration in the mammary blood compared to controls. The increase in the mammary blood lactate concentration could be due to the incapacity of the mammary cells to metabolize all the accumulated pyruvate that should be transformed into lactate, with the aim to produce NAD+ and continue the anaerobic energy production [15].…”

Section: Discussionmentioning

confidence: 99%

“…The APPs Hp and SAA were measured in the serum samples with validated methods for goats as previously recorded [15][16][17][18]. Serum concentrations of Hp were quantified by spectrophotometric method using a commercial kit based on the peroxidase activity of the haptoglobin-hemoglobin complex (Phase Haptoglobin Assay, Tridelta Development Ltd., Ireland).…”

Section: Hematology and Serum Biochemistrymentioning

confidence: 99%

Jugular versus Mammary Vein Acid-Base Balance, Blood Gases, Hematobiochemical Profiles and Inflammation Biomarkers in Goats with Acute Mastitis

Tharwat¹,

Al-Sobayil²,

Almundarij³

2017

Journal of Veterinary Science and Animal Husbandry

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Blood gas analyses, ruminal and blood pH, urine and faecal pH in dairy cows during subacute ruminal acidosis

et al. 2008

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Effect of time delay and storage temperature on blood gas and acid–base values of bovine venous blood

Cited by 26 publications

References 10 publications

Effects of blood storage on ice in biochemical and arterial blood gas analysis of rats

Effects of blood storage on ice in biochemical and arterial blood gas analysis of rats

Jugular versus Mammary Vein Acid-Base Balance, Blood Gases, Hematobiochemical Profiles and Inflammation Biomarkers in Goats with Acute Mastitis

Blood gas analyses, ruminal and blood pH, urine and faecal pH in dairy cows during subacute ruminal acidosis

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