Effect of time interval from insemination to first cleavage on the development of bovine embryos in vitro and in vivo

Lonergan, Patrick; Piumi, François; Humblot, P.

doi:10.1016/s0093-691x(99)91885-x

Cited by 4 publications

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“…In the non-superovulated cow the first in vivo cleavage takes place at 24-28 hr after ovulation (Thibault et al, 1987). According to earlier observations the first cleavage of bovine zygotes in vitro occurs between 24-48 hr post insemination (Barnes and Eyestone, 1990;Van Soom et al, 1992;Yadav et al, 1993;Lonergan et al, 1999;Rieger et al, 1999). In our study only a few zygotes (3%) cleaved before 24 hr post insemination and the vast majority (67%) cleaved for the first time between 24-30 hr post insemination.…”

Section: Discussionsupporting

confidence: 54%

“…The time of the first cleavage postinsemination has major, long-lasting effects on the subsequent development of the embryos. The rate of blastocyst development and the cell number of the resulting embryos are related to the time of the first cleavage (Yadav et al, 1993;Plante et al, 1994;Lonergan et al, 1999;Rieger et al, 1999). Indirect evidence suggests that fast-developing embryos are more likely to survive cryopreservation with establishment of pregnancies following embryo transfer (Hasler et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Timing of the first cleavage post‐insemination affects cryosurvival of in vitro–produced bovine blastocysts

Dinnyés¹,

Lonergan²,

Fair³

et al. 1999

Mol. Reprod. Dev.

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The time of the first cleavage of bovine zygotes during in vitro culture can affect the rate of development and cell number of the blastocysts. The aim of this work was to study the effect of the timing of first cleavage on the cryosurvival of the resulting blastocysts. Following standard IVM and IVF, zygotes were cultured in modified synthetic oviduct fluid (SOF), with 10% fetal calf serum (FCS) added 48 hr post insemination, in a humidified atmosphere of 5% CO 2, 5% O 2 and 90% N 2. Embryos which cleaved by 24, 27 30, 33, or 36 hr after insemination (IVF) were harvested and further cultured to the blastocyst stage (day 7 or day 8 post IVF). All developing blastocysts on days 7 and 8 were classified into three groups and were cryopre-served by vitrification. Group A consisted of blastocysts (150 m, small blastocysts); group B consisted of expanded or hatching blastocysts (150 m, large blastocysts); and group C consisted of morphologically poor quality blastocysts. The vitrification solution consisted of 6.5 M glycerol and 6% bovine serum albumin in PBS (VS3a). Thawed embryos were cultured further and survival was defined as the re-expansion and maintenance of the blastocoel over 24, 48, and 72 hr, respectively. Overall survival and hatching at 72 hr post-thawing was higher in blastocysts formed by day 7 than those formed by day 8 (60% vs. 40% survival; 63% vs. 45% hatching). Large blastocysts from day-7 and day-8 groups survived significantly better than small or poor quality blastocysts (76% vs. 63% and 31%; 72% vs. 30% and 26%, respectively; P 0.05). Day-7 blasto-cysts from the 27-and 30-hr cleavage groups survived significantly better than those from the 36-hr group (63% and 66% vs. 25%, P 0.05). Day-8 blastocysts from later cleaved (30 hr) zygotes had a higher survival than the 27-hr cleavage groups (52% vs. 26%, P 0.05). These results indicate that the day of blastocyst appearance, developmental stage, and timing of the first cleavage post-insemination can influence the cryo-survival of bovine blastocysts following vitrification.

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Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Timing of the first cleavage post‐insemination affects cryosurvival of in vitro–produced bovine blastocysts

Dinnyés¹,

Lonergan²,

Fair³

et al. 1999

Mol. Reprod. Dev.

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“…In particular, the developmental rate to the blastocysts stage was significantly higher for embryos that cleaved early after 24-30 h of insemination than those of embryos that cleaved slowly [21][22][23]. Besides, it has also been reported that the blastocyst formation rates of IVF embryos were different according to the developmental stage after 60 h of insemination; the time at which the blastocyst formation rate was checked was later than that reported above [27].…”

Section: Discussionmentioning

confidence: 82%

“…The reason for this improvement must have been the selection of IVF embryos used as donor embryos. To our knowledge, this is the first to report showing that the same pregnancy rates can be obtained after ET of NT embryos produced by using IVF and in vivo derived embryos as the source of donor blastomeres.Many reports have described that the timing of first cleavage after IVF has a major effect on subsequent in vitro development of embryos [21][22][23][24][25][26]. In particular, the developmental rate to the blastocysts stage was significantly higher for embryos that cleaved early after 24-30 h of insemination than those of embryos that cleaved slowly [21][22][23].…”

mentioning

confidence: 99%

Improvement of the Developmental Ability of Nuclear Transfer Embryos by Using Blastomeres from In Vitro Fertilized Embryos Selected According to the Early Developmental Stage and Cell Division Status as Donor Cells in Cattle

Goto

Matoba

Imai

et al. 2011

Journal of Reproduction and Development

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Abstract. This study was conducted to improve the developmental ability of nuclear transfer (NT) embryos by using blastomeres from in vitro fertilized (IVF) embryos with high quality as donor cells. The IVF embryos selected at the 2-cell stage at 24-h postinsemination (hpi) and again at the ≥8-cell stage at 48 hpi (Selected-IVF-embryos) showed the highest blastocyst formation rate among embryos. When blastomeres from the Selected-IVF-embryos (Selected-NT group) or Nonselected-IVF-embryos (Non-selected-NT group) were used as donor cells for NT, the blastocyst formation rate in the Selected-NT group (25.6%) was significantly higher than that in the Non-selected-NT group (13.5%). When blastomeres from the Selected-IVF-embryos at 108 (contained many cells before cell division) and 126 hpi (contained many cells immediately after cell division) were used as donor cells for NT (108-and 126-NT groups, respectively), the 126-NT group showed a significantly higher blastocyst formation rate (32.1%) than the 108-NT group (16.8%). Embryo transfer of blastocysts in the 126-NT group showed that 11 of 23 recipients became pregnant; nine calves were obtained. For the NT embryos reconstructed using in vivo derived embryos, 9 of 20 recipients became pregnant; seven calves were obtained. These results indicate that the blastocyst formation rate of NT embryos can be improved by using blastomeres from IVF embryos selected at the early developmental stage, especially immediately after cell division, and that the resultant NT embryos have a high developmental ability to progress to term that is comparable to NT embryos reconstructed using in vivo derived embryos. Key words: Bovine, Embryo, In vitro fertilization, Nuclear transfer (J. Reprod. Dev. 57: [249][250][251][252][253][254][255] 2011) uclear transfer (NT) technologies are now widely used by numerous laboratories to produce clone animals from embryonic and somatic cells. However, the overall efficiency of clone animal production is influenced by the different sources of donor cells; early pregnancy losses of cattle established from somatic nuclear transfer (sNT) are commonly above 50% [1,2], and the frequency of early fetal mortality is about two times higher with sNT than with embryonic nuclear transfer [3]. Therefore, it can be expected that a higher proportion of transferred NT embryos reconstructed from less-differentiated embryonic blastomeres would result in viable offspring compared with sNT. In addition, the production of embryonic cloned calves is permitted in Japan and food products derived from NT cattle can be on the market, thereby increasing commercial acceptance of the technology. Furthermore, the use of blastomeres from the same embryos for nuclear transfer to produce numerous identical bovine offspring is of great value for multiplication of genotypes having a superior economic value and is expected to contribute to their genetic improvement.In NT technology using embryos at an early developmental stage as donor cells, in vitro fertilized (IVF) embryos are oft...

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Timing of the first cleavage post-insemination affects cryosurvival of in vitro-produced bovine blastocysts

Dinnyés¹,

et al. 1999

View full text Add to dashboard Cite

The time of the first cleavage of bovine zygotes during in vitro culture can affect the rate of development and cell number of the blastocysts. The aim of this work was to study the effect of the timing of first cleavage on the cryosurvival of the resulting blastocysts. Following standard IVM and IVF, zygotes were cultured in modified synthetic oviduct fluid (SOF), with 10% fetal calf serum (FCS) added 48 hr post insemination, in a humidified atmosphere of 5% CO2, 5% O2 and 90% N2. Embryos which cleaved by 24, 27 30, 33, or 36 hr after insemination (IVF) were harvested and further cultured to the blastocyst stage (day 7 or day 8 post IVF). All developing blastocysts on days 7 and 8 were classified into three groups and were cryopreserved by vitrification. Group A consisted of blastocysts (<150 μm, small blastocysts); group B consisted of expanded or hatching blastocysts (>150 μm, large blastocysts); and group C consisted of morphologically poor quality blastocysts. The vitrification solution consisted of 6.5 M glycerol and 6% bovine serum albumin in PBS (VS3a). Thawed embryos were cultured further and survival was defined as the re‐expansion and maintenance of the blastocoel over 24, 48, and 72 hr, respectively. Overall survival and hatching at 72 hr post‐thawing was higher in blastocysts formed by day 7 than those formed by day 8 (60% vs. 40% survival; 63% vs. 45% hatching). Large blastocysts from day‐7 and day‐8 groups survived significantly better than small or poor quality blastocysts (76% vs. 63% and 31%; 72% vs. 30% and 26%, respectively; P < 0.05). Day‐7 blastocysts from the 27‐ and 30‐hr cleavage groups survived significantly better than those from the 36‐hr group (63% and 66% vs. 25%, P < 0.05). Day‐8 blastocysts from later cleaved (30 hr) zygotes had a higher survival than the 27‐hr cleavage groups (52% vs. 26%, P < 0.05). These results indicate that the day of blastocyst appearance, developmental stage, and timing of the first cleavage post‐insemination can influence the cryosurvival of bovine blastocysts following vitrification. Mol. Reprod. Dev. 53:318–324, 1999. © 1999 Wiley‐Liss, Inc.

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Effect of time interval from insemination to first cleavage on the development of bovine embryos in vitro and in vivo

Cited by 4 publications

References 0 publications

Timing of the first cleavage post‐insemination affects cryosurvival of in vitro–produced bovine blastocysts

Timing of the first cleavage post‐insemination affects cryosurvival of in vitro–produced bovine blastocysts

Improvement of the Developmental Ability of Nuclear Transfer Embryos by Using Blastomeres from In Vitro Fertilized Embryos Selected According to the Early Developmental Stage and Cell Division Status as Donor Cells in Cattle

Timing of the first cleavage post-insemination affects cryosurvival of in vitro-produced bovine blastocysts

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