Effect of time of gestation on fatty acid transporters mrna expression in bovine placenta

Abstract

ABSTRACT:The objective of the study was to evaluate the effect of time of gestation on fatty acid transporter mRNA expression in maternal and fetal bovine placenta. Placentas from twelve cows at different thirds of gestation (n=4 per third) were sampled at slaughter to measure FATP-1, FATP-4, FABP-1 mRNA concentration in maternal (caruncles) and fetal (cotyledons) side. Once the placenta was removed, 1cm 2 was dissected and, divided into caruncles and cotyledons, stored in sterile tubes, dropped into liquid nitrogen and kept at -80° C until rtPCR analysis. Data were analyzed as a complete randomized design with a 3 x 2 factorial arrangement, using the mixed procedure (SAS 9.3) with repeated measurements on space. Time of gestation, side of the placenta and their interaction were fixed factors, whereas animal was a random factor. There was a time by treatment interaction (P < 0.01) on FATP-1 mRNA expression due to a greater mRNA expression in cotyledons on the first third of gestation as compared with the concentration in caruncles. On the second and third stages of gestation, the mRNA concentration in cotyledons decreased, reaching a similar concentration to that observed in caruncles. Fatty acid transport protein -4 and FABP-1 mRNA concentration were not different (P >0.1). We conclude that FATP-1 might play an important role in fatty acid transport during early fetal development.

Eicosapentaenoic and docosahexaenoic acid supplementation during early gestation modified relative abundance on placenta and fetal liver tissue mRNA and concentration pattern of fatty acids in fetal liver and fetal central nervous system of sheep

“…Supplementing the maternal diet of ewes during the first 45 days of gestation with EPA and DHA tended to result in a greater abundance of FATP-1 mRNA transcript in the FL. The presence of FATP-1 was reported by Desantadina et al [27] during the initial two thirds of the gestation period in the caruncle area of the placenta of cattle and this finding is consistent with those of the present study. Feeding of diets containing greater amounts of fat lead to greater abundances of placental FATP-1 mRNA transcript compared with what is present in the placenta of animals fed standard diets [28], which may lead to increased transport of dietary fatty acids from the dam to the fetus.…”

Effects of maternal dietary omega-3 polyunsaturated fatty acids and methionine during late gestation on fetal growth, DNA methylation, and mRNA relative expression of genes associated with the inflammatory response, lipid metabolism and DNA methylation in placenta and offspring’s liver in sheep

“…On the other hand, our result on FATP1 relative mRNA expression differs from the ones reported by Roque-Jimenez et al [ 11 ], where an increase in the relative abundance of this gene was observed after maternal omega-3 PUFA supplementation during early gestation in fetal liver; but are in accordance with what was reported by Nickles et al [ 3 ] when PUFA supplementation was fed in the last third of gestation, even though a Met supplementation was not used in this trial. Desantadina et al [ 64 ] observed FATP1 in bovine maternal side of placenta in the first two thirds of gestation. The changes observed in FATP1 relative mRNA expression suggest that FATP1 action in FA metabolism may be more important in early gestation.…”

N-3 Polyunsaturated Fatty Acids as a Nutritional Support of the Reproductive and Immune System of Cattle—A Review

“…In ruminant animals, it has been observed that the transfer of some unsaturated fatty acids appears to be restricted in the epithelial-vascular placenta, often resulting in a deficiency of these components in newborns [130]. In a study conducted by Desantadina et al [154] on the placenta of cattle, changes in mRNA expression were noted only for the fatty acid transport protein FATP-1. In early pregnancy, FATP-1 shows a higher expression on the fetal side compared to the maternal side.…”