Effect of tissue-harvesting site on yield of stem cells derived from adipose tissue: implications for cell-based therapies

Jurgens, Wouter J.F.M.; Oedayrajsingh-Varma, Maikel; Helder, Marco N.; Zandieh-Doulabi, Behrouz; Schouten, Tabitha; Kuik, Dirk J.; Ritt, Marco J.P.F.; Milligen, F.J. van

doi:10.1007/s00441-007-0555-7

Cited by 338 publications

(273 citation statements)

References 43 publications

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“…After isolation of the ASCs from the SVF, we also found that the outer thigh region contained higher concentration of ASCs, compared to the abdomen, waist and the inner knee. This is in contrast to a previous study in which the abdomen has appeared as a preferable region for harvesting adipose tissue due to the higher ASCs yield (8).…”

Section: Discussioncontrasting

confidence: 87%

“…In particular, preclinical studies have revealed their capacity to differentiate toward the osteogenic, adipogenic, myogenic, and chondrogenic lineages (6,7). Nevertheless, adipose tissue displays a significant heterogeneity in terms of stem cell yield, proliferation, and differentiation capacity not only among individuals, but even when comparing different fat depots within one individual (8).…”

Section: Thigh Showed a Significantly Higher Number Of Ascs Compared mentioning

confidence: 99%

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Comparison of the Viability and Yield of Adipose-Derived Stem Cells (ASCs) from Different Donor Areas

Tsekouras

Mantas

Tsilimigras

et al. 2017

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Over the last few years, autologous fat grafting has been at the forefront of aesthetic plastic surgery clinical practice, since it provides exceptional results for the treatment of soft-tissue contour deformities (1, 2). Similar to bone marrow, the adipose tissue is derived from the embryonic mesenchyme and contains a supportive stromal vascular fraction (SVF) that can be easily isolated (3). Typically, SVF from adipose tissue contains different types of cells, including endothelial cells, smooth muscle cells, pericytes, leukocytes and pre-adipocytes, otherwise named as adipose-derived stem cells (ASCs) (4).The potential of ASCs to differentiate into diverse cell types both in vitro and in vivo, offers outstanding opportunities for their clinical application as therapeutic agents (5). In particular, preclinical studies have revealed their capacity to differentiate toward the osteogenic, adipogenic, myogenic, and chondrogenic lineages (6, 7). Nevertheless, adipose tissue displays a significant heterogeneity in terms of stem cell yield, proliferation, and differentiation capacity not only among individuals, but even when comparing different fat depots within one individual (8). 1229This article is freely accessible online.Correspondence to: Diamantis I. Tsilimigras, MD,

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Section: Discussioncontrasting

confidence: 87%

Section: Thigh Showed a Significantly Higher Number Of Ascs Compared mentioning

confidence: 99%

Comparison of the Viability and Yield of Adipose-Derived Stem Cells (ASCs) from Different Donor Areas

Tsekouras

Mantas

Tsilimigras

et al. 2017

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“…To exclude that stimulation with 5-aza-2-deoxycitidine also triggered the differentiation of ASC towards other lineages, we also tested our samples for markers for osteogenic and chondrogenic lineages at 5 weeks after treatment, as described previously (Jurgens et al 2008). No expression of the chondrogenic marker collagen type 2b was found in any of the samples.…”

Section: Resultsmentioning

confidence: 99%

Differentiation of human adipose-derived stem cells towards cardiomyocytes is facilitated by laminin

et al. 2008

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Adipose-derived stem cells (ASCs) are promising candidates for therapy in myocardial infarction (MI). However, the frequency of human ASCs that differentiate towards cardiomyocytes is low. We hypothesized that adherence to extracellular matrix molecules that are upregulated after MI might increase human stem cell differentiation towards cardiomyocytes. We analysed putative ASC differentiation on fibronectin-coated, laminincoated and uncoated culture plates. Expression of cardiac markers in cells was analysed 1, 3 and 5 weeks after stimulation with 5-aza-2-deoxycytidine. After 1 week, mRNA expression of myosin light chain-2α (MLC-2α), an early marker in cardiomyocyte development, was increased significantly in treated cells, independent of coating. At 5 weeks, however, mRNA expression of the late cardiomyocyte development marker SERCA2α was only significantly increased in 5-aza-2-deoxycytidine-treated cells cultured on laminin. Significantly higher numbers of cells were immunopositive for MLC-2α in cultures of treated cells grown on laminin-coated wells, when compared with cultures of treated cells grown on uncoated wells, both at 1 week and at 5 weeks. Furthermore, after 3 weeks, significantly more α-actinin-and desmin-positive cells were detected after treatment with 5-aza-2-deoxycytidine, but only in uncoated wells. After 5 weeks, however, the number of desmin-positive cells was only significantly increased after treatment of cells with 5-aza-2-deoxycytidine and culture on laminin (61% positive cells). Thus, we have found that a high percentage of human ASCs can be differentiated towards cardiomyocytes; this effect can be improved by laminin, especially during late differentiation.

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“…In addition, Jurgens et al [ 46 ] have recently demonstrated that not only differences exist among individuals, but also yield and functional features of hASCs are affected by the adipose tissue-harvesting site.…”

Section: Discussionmentioning

confidence: 99%

Anti-L-NGFR and -CD34 Monoclonal Antibodies Identify Multipotent Mesenchymal Stem Cells in Human Adipose Tissue

Quirici

Scavullo

Girolamo

et al. 2010

Stem Cells and Development

102

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Stem cells hold great promise in tissue engineering for repairing tissues damaged by disease or injury. Mesenchymal stem cells (MSCs) are multipotent cells able to proliferate and differentiate into multiple mesodermal tissues such as bone, cartilage, muscle, tendon, and fat. We have previously reported that the low-affi nity nerve growth factor receptor (L-NGFR or CD271) defi nes a subset of cells with high proliferative, clonogenic, and multipotential differentiation ability in adult bone marrow (BM). It has been recently shown that adipose tissue is an alternative source of adult multipotent stem cells and human adipose-derived stem cells, selected by plastic adherence (PA hASCs), have been extensively characterized for their functional potentials in vitro. In this study, immunoselected L-NGFR + and CD34 + subpopulations have been analyzed and compared with the PA hASCs. Phenotypic profi le of freshly purifi ed subpopulations showed an enrichment in the expression of some stem cell markers; indeed, a great percentage of L-NGFR + cells co-expressed CD34 and CD117 antigens, whereas the endothelial-committed progenitor markers KDR and P1H12 were mainly expressed on CD34 + cells. Differently from PA hASCs, the immunoseparated fractions showed high increments in cell proliferation, and the fi broblast colony-forming activity (CFU-F) was maintained throughout the time of culture. Furthermore, the immunoselected populations showed a greater differentiative potential toward adipocytes, osteoblasts, and chondrocytelike cells, compared to PA hASCs. Our data suggest that both CD34+ and L-NGFR + hASCs can be considered alternative candidates for tissue engineering and regenerative medicine applications. IntroductionI n the recent years, the emerging fi eld of cell-based therapies for repair and regeneration of damaged tissues has been focusing on the identifi cation of the ideal source of stem cells, which combines the ability of multipotential differentiation and the accessibility in large amounts under a minimally invasive procedure, not complicated by immunological rejection concerns and ethical controversies.Adult mesenchymal stem cells (MSCs) are a population of multipotent cells able to proliferate and differentiate into multiple mesodermal tissues. MSCs have been initially identifi ed in bone marrow (BM) [ 1 ], but have been subsequently isolated from many other tissues [ 2-10 ]. Among them, human MSCs derived from adipose tissue (hASCs) show stem cell key features such as the ability to form fi broblast-like colonies (CFU-F), the expression of several common cell surface antigens, the capacity of extensive proliferation, and the potential to differentiate in vitro and in vivo into multiple lineages of mesodermal origin, and also to transdifferentiate into neurogenic and hepatic lineages [ 4 , 11-21 ]. Similarly to BM-derived MSCs (BM MSCs), hASCs are able to suppress immunoreactivity, suggesting a possible overcoming of histocompatibility limitations in allogeneic transplantation [ 22 , 23 ]; furthermore, they ar...

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Effect of tissue-harvesting site on yield of stem cells derived from adipose tissue: implications for cell-based therapies

Abstract: The stromal vascular fraction

Cited by 338 publications

References 43 publications

Comparison of the Viability and Yield of Adipose-Derived Stem Cells (ASCs) from Different Donor Areas

Comparison of the Viability and Yield of Adipose-Derived Stem Cells (ASCs) from Different Donor Areas

Differentiation of human adipose-derived stem cells towards cardiomyocytes is facilitated by laminin

Anti-L-NGFR and -CD34 Monoclonal Antibodies Identify Multipotent Mesenchymal Stem Cells in Human Adipose Tissue

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