Effect of titanium surface roughness on chondrocyte proliferation, matrix production, and differentiation depends on the state of cell maturation

Schwartz, Zvi; Martin, J. Y.; Dean, David D.; Simpson, James; Cochran, David L.; Boyan, Barbara D.

doi:10.1002/(sici)1097-4636(199602)30:2<145::aid-jbm3>3.3.co;2-y

Cited by 40 publications

(55 citation statements)

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“…The implant surface can influence biological osteoconduction, bone formation and remodeling. Many studies have shown that during the sequence of these events, implant surface roughness has the best effect on cellular activity (Brunette 1988; Bowers et al 1992; Könönen et al 1992; Cochran et al 1994; Martin et al 1995; Schwartz et al 1996; Cooper et al 1999). Thus, implant roughness is important when immediate loading is to be applied (Salama et al 1995).…”

Section: Resultsmentioning

confidence: 99%

Influence of implant microstructure on the dynamics of bone healing around immediate implants placed into periodontally infected sites

Papalexiou¹,

Novaes²,

Grisi³

et al. 2004

Clinical Oral Implants Res

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This study evaluated by fluorescence analysis the influence of implant microstructure on the placement of immediate implants in periodontally infected sockets. Periodontal disease was induced during 12 weeks bilaterally from P1 to P4 in six dogs. The teeth were extracted and immediate implants were placed. Each dog received six implants: three with Friadent experimental surface (grit blasted/acid etched - FES group) and three covered with titanium plasma spray (TPS group), for a total of 36 Frialit-2 stepped cylinder implants. During the healing period, fluorescent bone markers were injected to study the bone remodeling around the implants. The dyes were injected in the following sequence: oxytetracyclin hydrochloride 3 days and 8 weeks, calcein green 4 weeks after the implantation and alizarin red S 3 days before killing. The animals were anesthetized and killed 12 weeks after implant placement. The mandibles were removed, dissected and processed for analyses of the percentage of newly formed bone surrounding the implant using a confocal laser scanning microscope. There were no significant statistical differences in bone formation (Mann-Whitney) between groups (FES group: 5.28% formation at 3 days, 10.3% at 4 weeks, 21.14% at 8 weeks and 6.98% at 12 weeks; TPS group: 3.36% at 3 days, 9.58% at 4 weeks, 14.57% at 8 weeks and 7.08% at 12 weeks). However, covariance analysis showed that the percent of marked bone was statistically greater for the FES group when compared to the TPS group, between the 3-day and 8-week periods of evaluation.

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Section: Resultsmentioning

confidence: 99%

Influence of implant microstructure on the dynamics of bone healing around immediate implants placed into periodontally infected sites

Papalexiou¹,

Novaes²,

Grisi³

et al. 2004

Clinical Oral Implants Res

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“…NHOst cells responded to surface roughness with a decrease in alkaline phosphatase activity, as do fetal rat calvaria cells13 and rat costochondral cartilage cells27 cultured on the same surfaces. In contrast, alkaline phosphatase is upregulated in chick embryo osteoblasts28 and is unaffected in mouse MLO‐Y4 osteocyte‐like cells13 by changes in surface roughness.…”

Section: Discussionmentioning

confidence: 99%

Response of normal female human osteoblasts (NHOst) to 17β‐estradiol is modulated by implant surface morphology

Lohmann

Tandy

Sylvia

et al. 2002

J. Biomed. Mater. Res.

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Titanium (Ti) surfaces with rough microtopographies enhance osteogenic differentiation, local factor production, and response to osteogenic agents in vitro and increase pullout strength of dental implants in vivo. Estrogens regulate bone formation, resorption, and remodeling in females and may be important in implant success. Here, we tested the hypothesis that estrogen modulates osteoblast response to implant surface morphology. Primary female human osteoblasts were cultured to confluence on three Ti surfaces (pretreatment, PT -R a 0.60 m; sandblasted and acid-etched, SLA -R a 3.97 m; and Ti plasma-sprayed, TPS -R a 5.21 m) and treated for 24 h with 10 −7 or 10 −8 M 17␤-estradiol (E 2 ). Cell number decreased with increasing surface roughness, but was not sensitive to E 2 . Alkaline phosphatase specific activity of isolated cells and cell layer lysates was lower on rough surfaces. E 2 increased both parameters on smooth surfaces, whereas on rough surfaces, the stimulatory effect of E 2 on alkaline phosphatase was evident only when measuring cell layer lysates. Osteocalcin levels were higher in the conditioned media of cells grown on rough surfaces; E 2 had no effect in cultures on the plastic surfaces, but increased osteocalcin production on all Ti surfaces. TGF-␤1 and PGE 2 production was increased on rough surfaces, and E 2 augmented this effect in a synergistic manner; on smooth surfaces, there was no change in production with E 2 . The response of osteoblasts to surface topography was modulated by E 2 . On smooth surfaces, E 2 affected only alkaline phosphatase, but on rough surfaces, E 2 increased levels of osteocalcin, TGF-␤1, and PGE 2 . These results show that normal adult human female osteoblasts are sensitive to surface microtopography and that E 2 can alter this response.

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“…The chemical and physical properties of scaffolds affect cellular behaviour, which ultimately determines the performance and fate of tissue‐engineered cartilage constructs (Boyan et al , 1996; Freed et al , 1993; Hutmacher, 2000). It has been demonstrated that cells on substrates with controlled topography exhibit different behaviour, suggesting that cells can distinguish the geometry of their surroundings (Boyan et al , 1996; Schwartz et al , 1996). Miot et al (2005) demonstrated that composition and architecture are instructive for expanded human nasal chondrocytes in the generation of three‐dimensional (3D) cartilaginous tissues.…”

Section: Introductionmentioning

confidence: 99%

Influence of pore size on the redifferentiation potential of human articular chondrocytes in poly(urethane urea) scaffolds

Stenhamre

Nannmark

Lindahl

et al. 2010

J Tissue Eng Regen Med

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The chemical and physical properties of scaffolds affect cellular behaviour, which ultimately determines the performance and outcome of tissue-engineered cartilage constructs. The objective of this study was to assess whether a degradable porous poly(urethane urea) scaffold could be a suitable material for cartilage tissue engineering. We also investigated whether the post-expansion redifferentiation and cartilage tissue formation of in vitro expanded adult human chondrocytes could be regulated by controlled modifications of the scaffold architecture. Scaffolds with different pore sizes, < 150 µm, 150-300 µm and 300-500 µm, were seeded with chondrocytes and subjected to chondrogenic and osteogenic induction in vitro. The poly(urethane urea) scaffold with the smaller pore size enhanced the hyaline-like extracellular matrix and thus neocartilage formation. Conversely, the chondrocytes differentiated to a greater extent into the osteogenic pathway in the scaffold with the larger pore size. In conclusion, our results demonstrate that poly(urethane urea) may be useful as a scaffold material in cartilage tissue engineering. Furthermore, the chondrogenic and the osteogenic differentiation capacity of in vitro expanded human articular chondrocytes can be influenced by the scaffold architecture. By tailoring the pore sizes, the performance of the tissue-engineered cartilage constructs might be influenced and thus also the clinical outcome in the long run.

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Effect of titanium surface roughness on chondrocyte proliferation, matrix production, and differentiation depends on the state of cell maturation

Cited by 40 publications

References 0 publications

Influence of implant microstructure on the dynamics of bone healing around immediate implants placed into periodontally infected sites

Influence of implant microstructure on the dynamics of bone healing around immediate implants placed into periodontally infected sites

Response of normal female human osteoblasts (NHOst) to 17β‐estradiol is modulated by implant surface morphology

Influence of pore size on the redifferentiation potential of human articular chondrocytes in poly(urethane urea) scaffolds

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