Effect of TNF-alpha on LH and IGF-I modulated chicken granulosa cell proliferation and progesterone production during follicular development

Onagbesan, O. M.; Mast, Jan; Goddeeris, Bruno; Decuypere, Eddy

doi:10.1530/reprod/120.2.433

Cited by 25 publications

(6 citation statements)

References 20 publications

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“…Cell viability was evaluated by the Trypan blue method and was usually > 90%. The cells were resuspended in Medium 199 (M199) supplemented with 2 mmol/L glutamine (1×), 40 mmol/L sodium bicarbonate (1×), 1% (v/v) PSA (antibiotic–antimycotic solution) (Sigma, England), and 5% charcoal-stripped fetal bovine serum (FBS) (Gibco BRL, Paisley) as described by Onagbesan et al [19], and granulosa cells were seeded at 0.1 × 10 5 viable cells per well in 12-well plastic plates (Corning Inc., Corning). The cells were cultured for 24 h in a humidified atmosphere containing 5% CO 2 at 37°C to establish cell cultures.…”

Section: Methodsmentioning

confidence: 99%

FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens

Cui

Han

Yin

et al. 2019

BioMed Research International

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FOXO3, which encodes the transcription factor forkhead box O-3 (FoxO3), is a member of the FOXO subfamily of the forkhead box (FOX) family. FOXO3 can be negatively regulated by its phosphorylation by the PI3K/Akt signaling pathway and ultimately drives apoptosis when activated. In mammalian ovaries, the FOXO3 protein regulates atresia and follicle growth by promoting apoptosis of ovarian granulosa cells. Nonetheless, the specific effects of the FOXO3 protein on granulosa apoptosis of avian ovaries have not been elucidated. Therefore, we studied FOXO3 expression in follicles with different organization and at all hierarchical levels of chicken follicles. Via an immunofluorescence assay, the chicken follicular theca at all hierarchical levels were found to be strongly stained with an anti-FOXO3 antibody. In chicken primary ovarian granulosa cells, mRNA levels of proapoptotic factors BNIP3 and BCL2L11 decreased in the absence of FOXO3, and so did PARP-1 and cleaved caspase 3 protein levels. After treatment with a recombinant FOXO3 protein, PARP-1 and caspase 3 protein levels increased, along with mRNA levels of Bnip3 and BCL2L11 (significantly, p<0.05). In addition, FOXO3 was downregulated in chicken granulosa cells when different estradiol or FSH concentrations were applied. In conclusion, FOXO3 is expressed in chicken reproductive tissues, including follicles and ovarian granulosa cells, and promotes apoptosis of chicken ovarian granulosa cells.

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Section: Methodsmentioning

confidence: 99%

FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens

Cui

Han

Yin

et al. 2019

BioMed Research International

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“…Postovulatory and atretic follicles had the greatest number of macrophages (43,45). Onagbesan et al (46) reported that conditioned media collected from LPS stimulated HD-11 avian macrophage cell line cultures mimicked the effects of TNF-a and its interactions with insulin-like growth factor-1 and luteinizing hormone on progesterone production and cell proliferation in cultured granulosa cells. Recently, Cornax et al (47) showed that primary chicken macrophages quickly phagocytized yolk after in vitro exposure without increases in inflammatory cytokines, such as IL-1, IL-6, and IFN-g.…”

Section: Ovulationmentioning

confidence: 99%

Obesity-Induced Dysfunctions in Female Reproduction: Lessons from Birds and Mammals

Walzem

Chen

2014

Advances in Nutrition

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Follicle wall rupture and ovum release, i.e., ovulation, has been described as a controlled inflammatory event. The process involves tissue remodeling achieved through leukocyte-mediated proteolysis. In birds, ovulation is the first step in the energy-intensive process of egg formation, yet hens that consume energy in excess of productive requirements experience impaired egg-laying ability. Broiler chickens, selected for rapid lean muscle gain, and coincidentally hyperphagia, develop adult obesity when given free access to feed. Obese broiler hens experience elevated circulating concentrations of insulin and leptin, changes in lipid and lipoprotein metabolism similar to those of human metabolic syndrome, as well as increased systemic inflammation. Overall, the manifestations in poultry are similar to those of women with polycystic ovary syndrome. It was shown recently that, in hens, as in mammals, changes in lipid synthesis and metabolism cause granulosa cell apoptosis and altered immune function and hormone production, further compromising ovarian function. To date, there is insufficient information on the means used by the ovary to direct leukocyte function toward successful ovulation. More information is needed regarding the control of proteolytic actions by leukocytes with regards to the roles of specific enzymes in both ovulation and atresia. The broiler hen has provided unique insight into the interrelations of energy intake, obesity, leukocyte function, and reproduction. Additional work with this model can serve the dual purposes of improving avian reproduction and providing novel insights into polycystic ovary syndrome in women.

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“…IGF1 is a well-known regulator of mammalian follicle development [15,16,[30][31][32][33]. Its role in preovulatory avian follicle development has received limited attention [14,18,19] and little is known about its effect on prehierarchal follicles. This study investigated the role of IGF1 on prehierarchal follicle function in the laying hen and as a model for broiler breeder hens that have increased IGF1 bioavailability when fed ad libitum [21].…”

Section: Discussionmentioning

confidence: 99%

“…Compared to mammals, the role of IGF1 in avian species is not well defined, and much of the research has been conducted using larger preovulatory follicles. In chicken preovulatory follicles, IGF1 has been shown to increase granulosa cell STAR, CYP11A1, and 3 β HSD protein expression [14], INHA mRNA expression [17], production of progesterone (the primary steroid hormone produced by avian granulosa cells) [14, 18, 19] and inhibin A [19], and cell proliferation [14, 18]. IGF1 has also been shown to have an additive effect with LH or FSH on progesterone and inhibin A production [18, 19].…”

Section: Introductionmentioning

confidence: 99%

Effect of IGF1 and FSH on the function of granulosa cells from prehierarchal follicles in chickens

Francoeur

Scoville

Johnson

2023

Biology of Reproduction

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IGF1 is an essential regulator of mammalian follicle development and synergizes with FSH to amplify its effects. In avian preovulatory follicles, IGF1 increases the expression of genes involved in steroidogenesis and progesterone and inhibin A production. The role of IGF1 in prehierarchal follicles has not been well studied in chickens. The aim of this study was to investigate the role of IGF1 in granulosa cells from prehierarchal follicles and to determine whether IGF1 and FSH synergize to promote follicle development. Granulosa cells of 3–5 mm and 6–8 mm prehierarchal follicles were cultured with IGF1 (0, 10, 100 ng/mL) in the presence or absence of FSH (0, 10 ng/mL). Cell proliferation, expression of genes important in follicle development (FSHR, IGF1R, AMH, STAR, CYP11A1, INHA, and INHBA), and progesterone production were evaluated following treatment. IGF1 treatment alone significantly increased STAR, CYP11A1, and INHBA mRNA expression and cell proliferation in granulosa cells of 6–8 mm follicles. IGF1 and FSH synergized to increase STAR mRNA expression in 6–8 mm follicles. IGF1 and FSH co-treatment were necessary to increase INHA mRNA expression in 6–8 mm follicles. Although IGF1 significantly increased the expression of genes involved in steroidogenesis, progesterone production in granulosa cells of 6–8 mm follicles was not affected. IGF1 did not affect AMH mRNA expression, although FSH significantly decreased AMH expression in granulosa cells of 3–5 mm follicles. These results suggest that IGF1 may act with FSH to promote follicle selection at the prehierarchal follicle stage.

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Effect of TNF-alpha on LH and IGF-I modulated chicken granulosa cell proliferation and progesterone production during follicular development

Cited by 25 publications

References 20 publications

FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens

FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens

Obesity-Induced Dysfunctions in Female Reproduction: Lessons from Birds and Mammals

Effect of IGF1 and FSH on the function of granulosa cells from prehierarchal follicles in chickens

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