Effect of TNF-α on Raji cells at different cellular levels estimated by various methods

Jurišić, Vladimir; Bogdanovic, Gordana; Kojić, Vesna; Jakimov, Dimitar; Srdic, Tatjana

doi:10.1007/s00277-005-0010-3

Cited by 48 publications

(32 citation statements)

References 53 publications

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“…We previously reported that patients with multiple myeloma have stage dependent increase of serum TNF-a that strongly correlated with tumor burden, percentage of plasma cell infiltration in bone marrow and presence of osteolyses [16]. TNF-a can modulate NK cell activity by inducing disturbance in cell metabolic activity following induction of apoptosis and necrosis, depending on TNF receptor system expression [17][18][19][20]. We show here, like in other patients with solid tumors (breast cancer and malignant melanoma) as well as in hematological diseases (Hodgkin's and non-Hodgkin's lymphoma) that multiple myeloma patient's show impairment of NK cell activity [3,4].…”

Section: Discussionmentioning

confidence: 99%

Clinical stage-depending decrease of NK cell activity in multiple myeloma patients

et al. 2007

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Natural killer cells, as an important subpopulation of cells of the innate immune system have an essential role in defense of the rise and spread of malignancy. These cells have a CD3-CD16 + CD56+ phenotype and they are functionally defined by their ability to lyses tumor cells. We here show that decrease of NK cell activity was significantly associated with advanced clinical stage, increased lactate dehydrogenase (LDH), percentage infiltration of bone marrow with plasma cells, and beta-2 microglobulin. The patients with higher NK cell activity at presentation after receiving VAD protocol have better cumulative survival in comparison with those with low NK cell activity.

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Section: Discussionmentioning

confidence: 99%

Clinical stage-depending decrease of NK cell activity in multiple myeloma patients

et al. 2007

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“…Tumour necrosis factor-α (TNF-α) is a cytokine initially identified as a cause of hemorrhagic necrosis in certain tumors (12,13). It is secreted by macrophages, monocytes and NK cells and many other cell types and it exists in two forms.…”

Section: Introductionmentioning

confidence: 99%

The Inflammatory Radicular Cysts Have Higher Concentration of TNF-α in Comparison to Odontogenic Keratocysts (Odontogenic Tumour)

Jurišić

Colic

Jurišić

2007

Acta Med. (Hradec Kralove, Czech Repub.)

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TNF-α is a pleiotropic cytokine that is considered as a primary modifier of inflammatory and immune reaction in response to various inflammatory diseases and tumour. We investigated levels of TNF-α in 43 radicular cysts and 15 odontogenic keratocysts, obtained from patients undergoing surgery, under local anaesthesia, and after aspiration of cystic fluid from non-ruptured cysts. TNF-α is elevated in both cysts’ fluid, but higher values were found in radicular cysts in comparison to keratocysts. The significantly higher concentration of TNF-α was associated with smaller radicular cysts, higher protein concentration, higher presence of inflammatory cells in peri cystic tissues, and the degree of vascularisation and cysts wall thickness (Mann-Whitney U-test, p<0.05). No correlation was found based on these parameters in odontogenic keratocyst, but all cysts have detectable concentrations of TNF-α. We here for the first time present that a difference in the concentration of TNF-α exists between these two cystic types.

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“…Medium was then discarded and 150 μl DMSO was added and samples were then shaken for 15 min. The absorbance was tested using a Microplate reader at 490 nm absorbance [18]. The inhibition rate was calculated using the following formula: the percentage of the MTT inhibition = (NC group OD 490 value -BANCR siRNA group OD 490 value)/ NC group OD 490 value.…”

Section: Mtt Assaysmentioning

confidence: 99%

BRAF-activated non-protein coding RNA (BANCR) advances the development of esophageal squamous cell carcinoma via cell cycle

Yang¹,

Wang²,

Chen

et al. 2017

Open Life Sciences

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Esophageal carcinoma is one of the important lethal tumors in the world, but the mechanism of esophageal cancer progression is still unclear. The long non-coding RNAs are known to regulate tumor progression. Here we report that BRAF-activated nonprotein coding RNA (BANCR) is associated with the development of esophageal squamous cell carcinoma (ESCC). The expression levels of BANCR in 132 ESCC tumor tissues detected by quantitative real-time PCR were significantly higher than that in the adjacent normal tissues. The expression level of BANCR was down-regulated by transfecting small interrupting RNA against BANCR (BANCR siRNA) in Eca109 cells. Eca109 and TE-1 cells transfected with BANCR siRNA led to the delayed cell cycles compared to the cells transfected with negative control siRNA. Moreover, MTT assays demonstrated that Eca109 cells transfected with BANCR siRNA resulted in the inhibition of cell proliferation. These results suggested that BANCR advances the development and progression of ESCC cells through regulating their cell cycles.

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Effect of TNF-α on Raji cells at different cellular levels estimated by various methods

Cited by 48 publications

References 53 publications

Clinical stage-depending decrease of NK cell activity in multiple myeloma patients

Clinical stage-depending decrease of NK cell activity in multiple myeloma patients

The Inflammatory Radicular Cysts Have Higher Concentration of TNF-α in Comparison to Odontogenic Keratocysts (Odontogenic Tumour)

BRAF-activated non-protein coding RNA (BANCR) advances the development of esophageal squamous cell carcinoma via cell cycle

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