Effect of topical application of Brazilian propolis on scratching behavior induced by compound 48/80 in mice

Abstract

From these results, it can be concluded that inhibition of scratching behavior induced by topical application occurred by both its local anesthetic and systemic action through inhibition of histamine release.

Anti-Allergic Properties of Propolis: Evidence From Preclinical and Clinical Studies

“…By contrast, chrysin and kaempferol were below detectable levels in Brazilian propolis, possibly explaining why Brazilian propolis showed weaker inhibitory effects on mast cell degranulation than Chinese propolis. Consistent with the finding reported by Nakamura et al (2010), a few other studies by the same group of researchers (Shinmei et al, 2004(Shinmei et al, , 2009(Shinmei et al, , 2010 have also demonstrated the inhibitory effects of Brazilian green propolis ethanol extract on histamine release in rat peritoneal mast cells induced with compound 48/80, a commonly used mast cell activator. The results of propolis in allergic diseases such as asthma, allergic rhinitis and atopic dermatitis in which mast cell degranulation plays a crucial role are further discussed in subsequent sections.…”

“…The inhibitory effects of propolis increased gradually when the treatment was continued daily for 4 weeks. Similar therapeutic effects of Brazilian green propolis for atopic dermatitis were demonstrated by another study by Shinmei et al (2010), which assessed the efficacy of topical propolis to relieve itch in compound 48/80-induced rats. Interestingly, Brazilian green propolis ethanol extract applied at a dose of 3 mg per site on skin 60 min before the inducer compound 48/80 was injected intradermally suppressed scratching behaviours.…”

“…Brazilian green propolis has been shown to ameliorate itching in animal models of atopic dermatitis when administered orally (Shinmei et al, 2004) or applied topically (Shinmei et al, 2010). A study by Shinmei et al (2004) assessed the effects of Brazilian green propolis granular on atopic dermatitis in rats induced with compound 48/80 or histamine.…”

“…As the chemical composition of propolis varies according to geographical locations, climates, botanical sources, and bee species (Katekhaye et al, 2019), it is difficult to relate the anti-allergic properties of propolis from different sources when the chemical composition is not known. However, several researchers have attributed the anti-allergic activities of propolis to the presence of flavonoids such as chrysin, kaempferol, galangin, and pinocembrin (Shinmei et al, 2009;Nakamura et al, 2010;Shinmei et al, 2010). These compounds have been demonstrated to have potential therapeutic effects for different allergic diseases in vivo, such as asthma (kaempferol, pinocembrin) (Gong et al, 2012;Gu et al, 2017), atopic dermatitis (chrysin) (Choi et al, 2017) as well as anaphylaxis (galangin) (Kim et al, 2013) (Figure 1).…”

Immunomodulatory Effects of Propolis and its Components on Basic Immune Cell Functions

“…Ten percent propolis treatment (1 mg/kg, once a day) inhibited the production of IgE, representative Th-2 cytokines, chemokines MCP-1 and TGF-β1, allergenspecific IgE, which resulted in inhibition of AHR (airway hyper-responsiveness) and reduced collagen deposition and the influx of inflammatory cells into the lungs of BALB/c mice challenged with allergens such as Dermatophagoides farine and diesel exhaust particles. In an experimental study by Shinmei et al [87] , propolis was given to rats for itchy nose and sneezing. The authors reported that symptoms were improved through decreased histamine release and that the longterm effects were favourable.…”