Effect of Total Parenteral Nutrition on Amino Acid and Glucose Transport by the Human Small Intestine

Inoue, Yasuhiro; Espat, N. Joseph; Frohnapple, David J.; Epstein, Howard; Copeland, Edward M.; Souba, Wiley W.

doi:10.1097/00000658-199306000-00002

Cited by 52 publications

(13 citation statements)

References 19 publications

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“…TPN-induced decreases in brush border hydrolase, nutrienttransporter activity, and microvillous height can be reversed with the provision of enteral nutrition within 1 week [15,16]. TPN has also been associated with an increase in mucosal permeability, an exaggerated response to endotoxin, and an increased incidence of pneumonia and sepsis [17][18][19].…”

Section: Discussionmentioning

confidence: 99%

Enterocutaneous Fistula and Small Bowel Evisceration of Twenty-five Years’ Duration: Successful Surgical and Nutritional Management

et al. 2007

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Section: Discussionmentioning

confidence: 99%

Enterocutaneous Fistula and Small Bowel Evisceration of Twenty-five Years’ Duration: Successful Surgical and Nutritional Management

et al. 2007

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“…The differing responses by diet treatment may be related to impairment of free amino acid uptake secondary to PN-induced intestinal atrophy (27); thus, the tempered response to free cysteine and glycine could be due to reduced amino acid availability.…”

Section: Articles Nosworthy and Bruntonmentioning

confidence: 99%

Cysteinyl-glycine reduces mucosal proinflammatory cytokine response to fMLP in a parenterally-fed piglet model

Nosworthy

Brunton

2016

Pediatr Res

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Background: PepT1 transports dietary and bacterial peptides in the gut. We hypothesized that cysteinyl-glycine would ameliorate the inflammatory effect of a bacterial peptide, formylmethionyl-leucyl-phenylalanine (fMLP), in both sow-fed and parenterally-fed piglets. Methods: An intestinal perfusion experiment was performed in piglets (N = 12) that were sow-reared or provided with parenteral nutrition (PN) for 4 d. In each piglet, five segments of isolated intestine were perfused with five treatments including cysteine and glycine, cysteinyl-glycine, fMLP, free cysteine and glycine with fMLP, or cysteinyl-glycine with fMLP. Mucosal cytokine responses and intestinal morphology was assessed in each gut segment. results: PN piglets had lower mucosal IL-10 by approximately 20% (P < 0.01). Cysteinyl-glycine lowered TNF-α response to fMLP in PN-fed animals and IFN-γ response to fMLP in both groups (P < 0.05). The free cysteine and glycine treatment reduced TNF-α in sow-fed animals (P < 0.05). fMLP affected villus height in parenterally (P < 0.05), but not sow-fed animals. conclusion: Parenteral feeding conferred a susceptibility to mucosal damage by fMLP. The dipeptide was more effective at attenuating the inflammatory response to a bacterial peptide than free amino acids. This may be due to competitive inhibition of fMLP transport or a greater efficiency of transport of dipeptides. t he products of protein digestion that are absorbed by the small intestinal epithelium include free amino acids and small peptides of two to three residues in length. These di/tripeptides are removed from the nutrient rich intestinal lumen by a H + /peptide symporter, peptide transporter 1 or PepT1 (1). This transporter is localized to the apical surface of the intestinal villi and has broad substrate specificity. Potential substrates for PepT1 include almost all possible dietary di/ tripeptides (1), some antibiotics (2-4) and proinflammatory bacterial peptides (5-8).The primary neutrophil chemotactic substance produced by Escherichia coli is formyl-methionyl-leucyl-phenylalanine (fMLP) (9). This tripeptide is the most predominant N-formylated peptide present in the colonic lumen of humans (9).PepT1-mediated transport of fMLP has been demonstrated in cell culture where uptake of fMLP was inhibited by the presence of known substrates of PepT1 (10,11). Further, the presence of fMLP induced neutrophil migration across an epithelial monolayer, an activity that was abolished if fMLP uptake was inhibited. PepT1-mediated transport of fMLP has also been demonstrated using in vivo rat models (12,13). In particular, uptake of fMLP has been investigated in rats using an intestinal perfusion approach (12). Marked inflammatory response occurred in jejunal segments perfused with fMLP, an intestinal position known to have high expression of PepT1. This inflammation was accompanied by an increase in DNA binding by NFκβ, a transcription factor involved in the regulation of proinflammatory cytokines. This transcription factor is capable of inducing transcri...

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“…Mean villous and crypt lengths were calculated from measures of 10 villi and 10 crypts selected at random from each sample. [16][17][18][19][20][21][22][23] Statistics Values of each measure were presented as mean þ SE or median and percentile. The significant difference of data between proximal and distal mucosae was tested using the Mann-Whitney U test.…”

Section: Morphometric Analysis Of the Proximal And Distal Mucosaementioning

confidence: 99%

Fecal Stream Is Essential for Adaptive Induction of Glucose-Coupled Sodium Transport in the Remnant Ileum After Total Proctocolectomy

Haneda

Fukushima

Funayama

et al. 2006

Journal of Gastrointestinal Surgery

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Effect of Total Parenteral Nutrition on Amino Acid and Glucose Transport by the Human Small Intestine

Cited by 52 publications

References 19 publications

Enterocutaneous Fistula and Small Bowel Evisceration of Twenty-five Years’ Duration: Successful Surgical and Nutritional Management

Enterocutaneous Fistula and Small Bowel Evisceration of Twenty-five Years’ Duration: Successful Surgical and Nutritional Management

Cysteinyl-glycine reduces mucosal proinflammatory cytokine response to fMLP in a parenterally-fed piglet model

Fecal Stream Is Essential for Adaptive Induction of Glucose-Coupled Sodium Transport in the Remnant Ileum After Total Proctocolectomy

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