Weakley, J, Johnston, RD, Cowley, N, Wood, T, Ramirez-Lopez, C, McMahon, E, and García-Ramos, A. The effects and reproducibility of 10, 20, and 30% velocity loss thresholds on acute and short-term fatigue and recovery responses. J Strength Cond Res XX(X): 000–000, 2023—This study aimed to establish the effects and reproducibility of implementing 10, 20, and 30% velocity loss thresholds (VLTs) during the free-weight barbell back squat on acute and short-term perceived soreness, neuromuscular fatigue, and physical performance. Using a repeated, counterbalanced, crossover design, 12 team-sport athletes completed on separate sessions 5 sets of the free-weight barbell back-squat until reaching VLTs of either 10, 20, or 30%. Outcomes were measured immediately postexercise and 24 hourS after each session. To assess reproducibility, the same sessions were repeated after 4 weeks. Immediately postexercise, small differences in countermovement jump (CMJ) and 10-m sprint performance were observed between VLT conditions, whereas small to moderate differences in differential ratings of perceived exertion were reported (10% < 20% < 30%). At 24 hours, trivial differences in CMJ outcomes were found but small differences in 10-m sprint performance were detected between conditions (10% < 20% < 30%). In addition, at 24 hours, a single small difference in radial deformation using tensiomyography was found between 10 and 30% conditions, whereas large to very large differences in perceived soreness were reported between conditions (10% < 20% < 30%). Finally, the standard error of measurement of all outcome measures at 24 hours were of a similar magnitude to those reported in tightly controlled, short-term studies. Collectively, these findings demonstrate that VLTs help control the fatigue outcomes that occur as a response to resistance training and that they are reproducible. Therefore, for practitioners who wish to prescribe resistance training and be confident in the subsequent fatigue response, it is strongly advised that VLTs are implemented.