Effect of transferrin concentration on bacterial growth in human ascitic fluid from cirrhotic and neoplastic patients

Romero, Álvaro Hidalgo; Pérez-Arellano, José-Luis; González-Villaron, Luis; Brock, Jeremy H.; Muñoz‐Bellido, Juan Luis; Castro, S. De

doi:10.1111/j.1365-2362.1993.tb01289.x

Cited by 15 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is present in mouse peritoneal¯uid in concentrations of 0.29±1.3 mg/ml (Barber et al 1990;Romero et al 1993) that are comparable to those supporting in vitro growth of T. foetus in an iron-deprived medium (Tachezy et al 1996). Transferrin is also present in the luminal¯uid of bovine uterus, a principal site of T. foetus infection (Roberts and Parker 1974).…”

Section: Discussionmentioning

confidence: 85%

Iron enhancement of experimental infection of mice by Tritrichomonas foetus

Kulda¹,

Poislová²,

Suchan³

et al. 1999

Parasitology Research

View full text Add to dashboard Cite

The ability of a microbial invader to acquire iron from its vertebrate host has been recognized as an important virulence mechanism in some pathogenic bacteria. We examined the involvement of similar mechanisms in an experimental infection of mice by a protozoan pathogen of cattle, Tritrichomonas foetus. In a series of experiments, outbred ICR mice were inoculated intraperitoneally with two strains of T. foetus, the moderately virulent KV-1 (approximately 5% mortality rate) and the highly virulent LUB-1MIP (approximately 80% mortality rate). Treatment of mice with ferric ammonium citrate (FeAC) (100 mg/kg per day intraperitoneally) increased the mortality rate caused by the KV-1 infection up to the level determined for the highly virulent strain. The treatment effect was dose dependent and required early administration of FeAC after inoculation of parasites and its continued supply for at least 3 subsequent days. Daily sampling of peritoneal exudate showed that the infection-enhancing effect of iron overload was associated with a stimulation of parasite multiplication, which in the case of KV-1 infection was strongly suppressed in untreated mice. Consistent with these findings, the strain of lower virulence (KV-1) showed considerably lower efficiency accumulating radiolabeled iron from transferrin and a low-molecular source [Fe(III)nitrilotriacetic acid] in vitro. The results indicate an involvement of iron uptake mechanisms by the parasite as a virulence factor in T. foetus infection.

show abstract

Section: Discussionmentioning

confidence: 85%

Iron enhancement of experimental infection of mice by Tritrichomonas foetus

Kulda¹,

Poislová²,

Suchan³

et al. 1999

Parasitology Research

View full text Add to dashboard Cite

show abstract

“…Moreover, mice administrated with excess amount of iron die in early stage of Candida infection [2][3][4]. Thus, transferrin has been thought to play an important role in protection against fungal and bacterial infections [21]. The protective mechanism of transferrin is due to a strong chelating activity for ferrous ion resulting in the deficiency of ferrous ion required for the growth of microbes including fungi.…”

Section: Discussionmentioning

confidence: 99%

Candida albicans, Cryptococcus neoformans or Aspergillus fumigatus Induces an Antifungal Activity in Mouse Serum, which is Different from Transferrin

Okazaki

Asakura

Sugimoto

et al. 2009

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. It has been reported that administration of Candida albicans into mouse induces an antifungal activity in serum, which has been identified as transferrin. In the present study, we show that not only C. albicans, but also other fungus such as Cryptococcus neoformans or Aspergillus fumigatus similarly can induce an antifungal activity in mouse serum. This antifungal activity was inhibited by the addition of ferrous ion, indicating that the growth inhibition of C. albicans was due to deficiency of ferrous ion, which may be caused by transferrin. Indeed, addition of transferrin in an in vitro assay system using RPMI1640 culture medium inhibited the growth of C. albicans, C. neoformans or A. fumigatus. However, when C. albicans was grown in RPMI1640 medium with 10% fetal bovine serum (FBS), transferrin was unable to inhibit the growth of C. albicans, in sharp contrast, when C. albicans treated mouse serum was added instead of FBS, the growth of the organism was inhibited. Similar results were obtained when C. neoformans or A. fumigatus was used. Taken together, the results suggest that antifungal activity induced by C. albicans, C. neoformans or A. fumigatus was not due to transferrin but likely due to other unknown serum proteins, which may cut off the source of iron for the growth of these fungi.

show abstract