Effect of Transportation on the Posttransfusion Survival of Blood Stored in CPD

Abstract

Studies on the effects of land and air transpnrtation on CPD blood stored for 21 and 28 days are reported. Plasma potassium, supernatant hemoglobin, per cent hemolysis, whole blood pH, plasma sugar, posttransfusion survival (autologous) and t / 2 of the transported units did not differ significantly frcim thosr nf the control units that were left undisturbed in the refrigerator for comparable 1. Clin. Invest. 26: 687, 1947.

The quality of over- and undercollected blood for transfusion

“…This 2. 9 per cent loss if extrapolated to our national annual blood collection of eight million blood units represents a loss of 232,000 units.…”

The Development and Acceptance of the Anticoagulant, CPD

“…The FDA, mindful of the massive use of red cells shipped from the United States mainland to support the treatment of military personnel and civilians during the Korean conflict, requested that a study be made of the effect of travel upon packed red cells stored in ice for 24 h. T o obtain this information, we either hand-carried blood by train to Richmond, Va. and back, or shipped blood by air from Chicago to Boston and promptly returned it to Chicago for estimation of the red cell survival and the in vitro characteristics ofthe red cells. There were no significant differences in the in vivo and in vitro characteristics of the shipped units when they were compared to those of the control units [9].…”

“…2 children, following transfusion of 28-day-old red cells, developed transient jaundice [8]. This evidence prompted a study of the prolonged storage of hard-packed CPD red cells with hematocrits averaging 85% [9] that showed their average survival to be well below the 70% criterion. Therefore, the acceptable storage time for separated red cells was established at 21 days.…”

The Development and Acceptance of the Anticoagulant, CPD

“…There were no significant differences in the in vivo and in vitro characteristics of the shipped units when they were compared to those of the control units [9], These laboratory and clinical data proved sufficient to warrant the FDA to permit CPD to be used for the collection and storage of whole blood and separated red cells for 21 days after collection. cells were first collected into heparin, and then added to a series of tubes containing increasing amounts of ACD to provide a series of blood aliquots with blood-to-ACD ratios ranging from 20% of the accepted amount of blood to the proper 1:15 ratio of ACD-to-whole blood [5].…”

“…2 children, follow ing transfusion of 2 8-day-old red cells, de veloped transient jaundice [8]. This evi dence prompted a study of the prolonged storage of hard-packed CPD red cells with hematocrits averaging 85% [9] that showed their average survival to be well below the 70% criterion. Therefore, the acceptable storage time for separated red cells was es tablished at 21 days.…”