Effect of treatment with enrofloxacin and Lactobacillus probiotics on ABCB1, ABCC2 and ABCG2 mRNA expression in poultry

Abstract

Poultry feed is often supplemented by Lactobacillus probiotics which may alter drug bioavailability by affecting the expression of intestinal ATP-binding cassette (ABC) efflux transporters. Therefore the effect of probiotics, administered alone or in combination with enrofloxacin, on the expression of ABCB1, ABCC2 and ABCG2 mRNAs in chickens was evaluated. Day-old Ross chicks (n=24) were divided in four equal groups. Control group was not treated. The second group received feed with addition of probiotics Lactobacillus brevis, L. plantarum and L. bulgaricus 5 days after hatching, for 15 days. The third group received probiotics as described above and enrofloxacin at the age of 15 days (10 mg/kg, via drinking water for 5 days). The last group received enrofloxacin at age of 15 days (10 mg/kg, via drinking water for 5 days). Expression of ABC transporters in liver, duodenum and jejunum was determined by qRT-PCR. Down-regulation of ABCG2 mRNA in the liver (P<0.05), its up-regulation in the duodenum (P<0.05) and increased ABCB1 mRNA levels in the jejunum (P<0.05) can be attributed to enrofloxacin treatment. Decrease in ABCC2 mRNA expression in the duodenum can be associated with enrofloxacin administration. The observed changes were related to enrofloxacin administration and to lesser extent to Lactobacillus supplementation.

The effects of cyclosporine A or activated charcoal co-administration on the pharmacokinetics of enrofloxacin in chickens

“…The authors of this study observed that the drug was absorbed slower and to a lower extent in the infected birds which was related to the increased expression levels of ABCB1 mRNA, the gene encoding P-gp. Other studies carried out in poultry species found that danofloxacin and ENR treatments lead to the tendency for upregulation of ABCB1 and ABCG2 mRNA ( Haritova et al, 2008 ; Pavlova et al, 2018 ). The effect of P-gp on ENR pharmacokinetics was further proved at functional level in broilers by co-administration of verapamil, a potent and specific inhibitor of this transporter protein ( Guo et al, 2014 ).…”