Effect of treatment with simvastatin on bone microarchitecture of the femoral head in an osteoporosis animal model

Abstract

The objective of this study was to evaluate the microarchitecture and trabecular bone strength at the distal region of the femur, and its biomechanical properties with simvastatin administration with two different doses in ovariectomized (OVX) rats. Ninety rats were divided into six groups to evaluate treatment with the simvastatin drug (n = 15): SH (Sham surgery), SH-5 (5 mg simvastatin), SH-20 (20 mg simvastatin), OVX, OVX-5, and OVX-20. Euthanasia was performed at three different times, five animals per period: 7, 14, and 28 days. The effectiveness of the treatments was evaluated by mechanical testing and histomorphometric analysis of the femurs. The results of analysis by the linear model of mixed effects showed 20 mg of simvastatin results in increased trabecular bone after 14 days (P = 0.039) of ingestion in ovariectomized animals. However, ingestion of 5 mg of simvastatin is able to sensitize the trabecular bone only at 28 days (P = 0.005) of ingestion. In the mechanical tests stiffness improves within 28 days (P = 0.003). Regarding maximum strength, no statistical differences were observed. According to these results, it can be concluded that for a decrease in oral intake, longer treatment times are required. Microsc. Res. Tech. 79:684-690, 2016. © 2016 Wiley Periodicals, Inc.

The Effects of Tocotrienol and Lovastatin Co-Supplementation on Bone Dynamic Histomorphometry and Bone Morphogenetic Protein-2 Expression in Rats with Estrogen Deficiency

“…The lovastatin dose used in this study does not exceed the recommended statin dose for high-intensity hypocholesterolemic effects in humans (80 mg/day) [ 36 ]. Monteiro et al showed that oral supplementation of very-high-dose simvastatin (20 mg/kg/day, equivalent to 140 mg/day in humans) improved the bone microstructure of ovariectomized rats in 14 days [ 37 ]. Similar effects were not observed with a lower dose within the same treatment period [ 37 ].…”

“…Monteiro et al showed that oral supplementation of very-high-dose simvastatin (20 mg/kg/day, equivalent to 140 mg/day in humans) improved the bone microstructure of ovariectomized rats in 14 days [ 37 ]. Similar effects were not observed with a lower dose within the same treatment period [ 37 ]. Thus, it is reasonable that lovastatin at the dose used in the current study produced no effects on bone dynamic histormorphometric parameters in ovariectomized rats.…”

Treatment of experimental periodontal disease by laser therapy in simvastatin-modified rats

“…Animals were numbered and randomly divided into two groups: Veh group (n=90) received Polyethylene Glycol 400 (All Chemistry; São Paulo, SP, Brazil) at 0.5 mg/kg body weight (vehicle), and S group (n=90) received Simvastatin (Medley; Campinas, SP, Brazil) at 0.5 mg/kg body weight orally 18 . Administrations were daily performed in a single dose, starting 24 h before induction of PD and maintained until the end of the respective periods of euthanasia.…”

Estudo da ação da Doxiciclina em diferentes modelos de osteopenia

“…A quantificação de trabeculado ósseo por Tricromo de Masson foi expresso em Pixel² (ISSA et al, 2015;MONTEIRO et al, 2016), a quantificação por picro-sirius red foi expresso em porcentagem de colágeno total (%) (PEREIRA, B. F. et al, 2014) e a imunomarcação por TRAP foi expresso em Escores (SHIMANO, R. C. et al, 2014).…”

“…A qualidade óssea é determinada pela taxa de remodelação óssea que é um processo contínuo de renovação óssea, onde um tecido velho ou danificado é reabsorvido e um novo tecido é formado, mantendo assim a integridade mecânica do osso e a homeostase do cálcio no organismo. As propriedades estruturais dos ossos incluem sua geometria (tamanho e forma) e sua microarquitetura (trabecular ou cortical), enquanto suas propriedades materiais (ISSA et al, 2015;MACEDO et al, 2017;MONTEIRO et al, 2016;VOLPON;LIMA, 2008;SHIMANO, ROBERTA CARMINATI et al, 2014).…”