Effect of trehalose on the preservation of freeze-dried mice spermatozoa at room temperature

Ito, Daiyu; Wakayama, Sayaka; Kamada, Yuko; Shibasaki, Ikue; Kamimura, Satoshi; Ooga, Masatoshi; Wakayama, Teruhiko

doi:10.1262/jrd.2019-058

Cited by 22 publications

(20 citation statements)

References 33 publications

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“…When the average rate was compared between preservation periods, although the S6 group showed a slightly but significantly lower fertilization failure rate (8.4%) than the others (9.9 to 11.2%), the difference was not large. This rate was within the normal range when FD sperm were used (35,36). These results suggest that space radiation did not affect the cytoplasmic factors of spermatozoa.…”

Section: Detection Of Fd Sperm Damagesupporting

confidence: 60%

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Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station

et al. 2021

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Space radiation may cause DNA damage to cells and concern for the inheritance of mutations in offspring after deep space exploration. However, there is no way to study the long-term effects of space radiation using biological materials. Here, we developed a method to evaluate the biological effect of space radiation and examined the reproductive potential of mouse freeze-dried spermatozoa stored on the International Space Station (ISS) for the longest period in biological research. The space radiation did not affect sperm DNA or fertility after preservation on ISS, and many genetically normal offspring were obtained without reducing the success rate compared to the ground-preserved control. The results of ground x-ray experiments showed that sperm can be stored for more than 200 years in space. These results suggest that the effect of deep space radiation on mammalian reproduction can be evaluated using spermatozoa, even without being monitored by astronauts in Gateway.

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Section: Detection Of Fd Sperm Damagesupporting

confidence: 60%

“…The lever of the freeze-drying machine was opened for at least 3 hours until the samples became completely dry. After drying, the ampules were sealed by melting their necks using a gas burner under vacuum conditions, as described (31,35), and were kept in a −30°C freezer until further use.…”

Section: Preparation Of Fd Spermmentioning

confidence: 99%

Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station

et al. 2021

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“…ICSI was performed as previously described [ 12 , 13 ]. Briefly, for the microinjection of spermatozoa, 1–2 µl of the sperm culture solution were directly moved to the injection chamber.…”

Section: Methodsmentioning

confidence: 99%

Improvement of a twice collection method of mouse oocytes by surgical operation

Inoue

Harada

Wakayama

et al. 2020

Journal of Reproduction and Development

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Mouse oocytes are generally collected after euthanasia. However, if oocytes were collected without euthanasia, then mice could be used to collect oocytes again after recovery. This condition is especially useful for mice that are genotypically rare. In this study, we examined the reusability of mice after collecting oocytes via a surgical operation. When oocytes were collected using medetomidine/midazolam/butorphanol combination anesthesia and examined for the quality of oocytes after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), they could develop to full term at the same rate as controls. When oocytes were collected from those mice a second time, the average number of oocytes was reduced by nearly 1/3. However, the blastocyst and offspring rates of those oocytes after IVF or ICSI were the same as those of the control regardless of the recovery day period. Although germinal vesicle (GV) oocytes can be collected from all reused mice, the final number of offspring did not increase. Interestingly, when oocytes were collected from the front position of the ampulla, 76% of the oviducts possessed oocytes after reuse, and the average number of oocytes significantly increased to a level comparable to that of the control. Finally, we examined whether reused mice can be used as recipient females, and then healthy offspring were obtained similarly as the control recipients. In conclusion, we provide a new method to collect a sufficient number of oocytes from reused mice without concern.

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“…Membrane-permeable trehalose would, in theory, allow better control of its uptake (and removal) by changing concentrations and, even more, by longer exposure to trehalose gradients. Surprisingly, a recent report claimed that trehalose lacks any DNA protection activity (Ito et al 2019); given that this report comes from an authoritative group, alternative compounds with antioxidant activity, like rosmarinic acid or melatonin, should be explored, as suggested by Mercati et al (2020). However, in our opinion, natural xeroprotectants found in tardigrades, midges or other anhydrobiotic organisms should guide our choices.…”

Section: Future Directions: Spermatozoamentioning

confidence: 97%

Dry storage of mammalian spermatozoa and cells: state-of-the-art and possible future directions

Loi

Anzalone

Palazzese

et al. 2021

Reprod. Fertil. Dev.

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This review provides a snapshot of the current state-of-the-art of drying cells and spermatozoa. The major successes and pitfalls of the most relevant literature are described separately for spermatozoa and cells. Overall, the data published so far indicate that we are closer to success in spermatozoa, whereas the situation is far more complex with cells. Critical for success is the presence of xeroprotectants inside the spermatozoa and, even more so, inside cells to protect subcellular compartments, primarily DNA. We highlight workable strategies to endow gametes and cells with the right combination of xeroprotectants, mostly sugars, and late embryogenesis abundant (LEA) or similar ‘intrinsically disordered’ proteins to help them withstand reversible desiccation. We focus on the biological aspects of water stress, and in particular cellular and DNA damage, but also touch on other still unexplored issues, such as the choice of both dehydration and rehydration methods or approaches, because, in our view, they play a primary role in reducing desiccation damage. We conclude by highlighting the need to exhaustively explore desiccation strategies other than lyophilisation, such as air drying, spin drying or spray drying, ideally with new prototypes, other than the food and pharmaceutical drying strategies currently used, tailored for the unique needs of cells and spermatozoa.

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Effect of trehalose on the preservation of freeze-dried mice spermatozoa at room temperature

Cited by 22 publications

References 33 publications

Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station

Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station

Improvement of a twice collection method of mouse oocytes by surgical operation

Dry storage of mammalian spermatozoa and cells: state-of-the-art and possible future directions

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