Effect of Trichloroacetic Acid Treatment on Certain Properties of Spores of <i>Bacillus cereus</i> T

Shibata, Hirofumi; Uchida, Michiko; Hayashi, Hiroko; Tani, Isamu

doi:10.1111/j.1348-0421.1979.tb00471.x

Cited by 5 publications

(6 citation statements)

References 25 publications

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“…The extraction of spores with SDS-DTT at alkaline pH resulted in solubilization of a large amount of spore coat protein with alterations in the spore coat morphology showing complete or partial dissolution of the pitted and undercoat layers in addition to overall thinning of the coat (3). As compared to total protein of normal spores (29), about 30% was solubilized under the present extraction condition. This result is compatible with the data reported previously (3).…”

Section: Restoration By Calcium Of L-alanine-induced Germinabilities mentioning

confidence: 99%

Involvement of Calcium in Germination of Coat‐Modified Spores of Bacillus cereus T

Shibata

Miyoshi

Osato

et al. 1992

Microbiology and Immunology

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The effect of calcium on germination of coat-modified Bacillus cereus T spores was investigated. Coat-modified spores produced either by chemical extraction (SDS-DTT-treated spores) or by mutagenesis (10LD mutant spores) were unable to germinate in response to inosine. While SDS-DTT-treated spores could germinate slowly in the presence of L-alanine, l OLD mutant spores could not germinate at all. The lost or reduced germinability of coat-modified spores was restored when exogenous Ca2+ was supplemented to the germination media. The calcium requirement of coat-modified spores for germination was fairly specific. The simultaneous presence of germinant with Ca2+ was also required for germination of coat-modified spores. The optimal recovery of germinability was obsei ved in the presence of 1.0 mm of calcium acetate. The calcium requirement itself was remarkably diminished under the condition in which L-alanine and a certain purine nucleoside analog, adenosine or inosine, coexisted. The lost or diminished germinability observed in SDS-DTT-treated spores or l OLD mutant spores may be attributed to the loss of calcium associated with the spore integuments.

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Section: Restoration By Calcium Of L-alanine-induced Germinabilities mentioning

confidence: 99%

Involvement of Calcium in Germination of Coat‐Modified Spores of Bacillus cereus T

Shibata

Miyoshi

Osato

et al. 1992

Microbiology and Immunology

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“…Exposure of spores to trichloroacetic acid alters their viability and germinability and their response to heat and alkali but not to lysozyme (191). Trichloroacetic acid could help provide useful data about mechanisms of action of other agents.…”

Section: Mechanisms Of Sporicidal Actionmentioning

confidence: 99%

Bacterial spores and chemical sporicidal agents

1990

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Bacterial spores are among the most resistant of all living cells to biocides, although the response depends on the stage of sporulation. The development of resistance to some agents such as chlorhexidine occurs much earlier in sporulation than does resistance to glutaraldehyde, which is a very late event. During germination or outgrowth or both, resistance is lost and the cells become as susceptible to biocides as nonsporulating bacteria. Mechanisms of spore resistance to, and the action of, biocides are discussed, and possible means of enhancing antispore activity are considered. The clinical and other uses of sporicidal and sporostatic chemical agents are described.

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“…Spores of Bacillus and Clostridium species have long been known to be resistant to killing by both acid and alkali, compounds generally not used alone as disinfectants, but often as one component of disinfection regimens (Roberts and Hitchins 1969;Shibata et al 1979;Russell 1990;McDonnell and Russell 1999). Spores are also very resistant to ethanol, again a common disinfectant (Russell 1990).…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of killing spores of Bacillus subtilis by acid, alkali and ethanol

et al. 2002

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Aims: To determine the mechanisms of killing of Bacillus subtilis spores by ethanol or strong acid or alkali. Methods and Results: Killing of B. subtilis spores by ethanol or strong acid or alkali was not through DNA damage and the spore coats did not protect spores against these agents. Spores treated with ethanol or acid released their dipicolinic acid (DPA) in parallel with spore killing and the core wet density of ethanol-or acid-killed spores fell to a value close to that for untreated spores lacking DPA. The core regions of spores killed by these two agents were stained by nucleic acid stains that do not penetrate into the core of untreated spores and acid-killed spores appeared to have ruptured. Spores killed by these two agents also did not germinate in nutrient and nonnutrient germinants and were not recovered by lysozyme treatment. Spores killed by alkali did not lose their DPA, did not exhibit a decrease in their core wet density and their cores were not stained by nucleic acid stains. Alkali-killed spores released their DPA upon initiation of spore germination, but did not initiate metabolism and degraded their cortex very poorly. However, spores apparently killed by alkali were recovered by lysozyme treatment. Conclusions: The data suggest that spore killing by ethanol and strong acid involves the disruption of a spore permeability barrier, while spore killing by strong alkali is due to the inactivation of spore cortex lytic enzymes. Significance and Impact of the Study: The results provide further information on the mechanisms of spore killing by various chemicals.

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Effect of Trichloroacetic Acid Treatment on Certain Properties of Spores of Bacillus cereus T

Abstract: Spores of Bacillus cereus T

Cited by 5 publications

References 25 publications

Involvement of Calcium in Germination of Coat‐Modified Spores of Bacillus cereus T

Involvement of Calcium in Germination of Coat‐Modified Spores of Bacillus cereus T

Bacterial spores and chemical sporicidal agents

Mechanisms of killing spores of Bacillus subtilis by acid, alkali and ethanol

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