Effect of Trifluoroperazine and Sodium Decyl Sulfate on Posthypertensive Shock of Human and Rabbit Erythrocytes

Yershova, N.A.; Chabanenko, Olena; Shpakova, N. M.; Nipot, O.; Орлова, Н. В.

doi:10.15407/fz68.01.062

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…Її пов'язують зі здатністю збільшувати площу поверхні клітини при вбудовуванні амфіфільної сполуки. Оскільки пошкодження при постгіпертонічному шоці зумовлене перевищенням критичного гемолітичного об'єму, застосування амфіфільних сполук у певній концентрації може мати захисний ефект [5][6][7][8].…”

Section: вступunclassified

Evaluation of the Form and Distribution of Phosphatidylserine in Human Erythrocytes Exposed to Posthypertonic Shock Under the Protection of Amphiphilic Compounds

Nipot,

Yershova,

Chabanenko

et al. 2024

Fiziol Zh

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The search for protective substances that can be used during red blood cell thawing and the study of their effects on red blood cells contribute to increasing the number and quality of viable cells after the cryopreservation cycle. We studied the effect of posthypertonic shock and amphiphilic compounds on the shape and eryptosis of human erythrocytes. The method of flow cytometry was used, this allows analyzing two parameters simultaneously, which increases the efficiency of research. The shape was assessed by the sphericity index (SphI), and eryptosis by the redistribution of phosphatidylserine to the membrane outer surface. It has been shown that sodium decylsulfate and chlorpromazine reduce erythrocyte damage in posthypertonic shock by 3.6 and 4.2 times, respectively. Sodium decylsulfate helps to preserve the shape of cells (SphI coefficient remains the same), while when chlorpromazine is used, the shape changes towards spherical (SphI coefficient changes 2 times). The study of the level of Annexin V FITC binding to phosphatidylserine in outer layer of membrane revealed a concentration-dependent increase in fluorescence when sodium decylsulfate was used, indicating a disorder of the bilayer asymmetry. In contrast, chlorpromazine did not change the distribution of phosphatidylserine. Comparison of two parameters of cell viability - the sphericity coefficient and anexin binding - allowed us to choose the conditions that are optimal for the use of the studied protective substances. Namely, it is advisable to use the lowest effective concentration of sodium decylsulfate (200 mcmol/l) for protective purposes. This ensures the preservation of the cell shape and minimal impact on the membrane asymmetry.

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Section: вступunclassified

Evaluation of the Form and Distribution of Phosphatidylserine in Human Erythrocytes Exposed to Posthypertonic Shock Under the Protection of Amphiphilic Compounds

Nipot,

Yershova,

Chabanenko

et al. 2024

Fiziol Zh

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“…Другий механізм полягає в збільшенні швидкості дифузії катіонів через клітинну мембрану, внаслідок формування дефектів проникності, що знімає осмотичний стрес і запобігає гемолізу [12]. Відсутність витоку іонів калію під час інкубації еритроцитів з низькими концентраціями амфіфільних речовин свідчить на користь першого припущення [13].…”

Section: результати та їх обговоренняunclassified

The Role of Cell Energy Status in the Protection of Posthypertonic Hemolysis by Amphiphilic Substances

Nipot¹,

Yershova²,

Shapkina³

et al. 2022

Fiziol Zh

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The effect of ATP depletion on the protection of rabbit eryth- rocytes by amphiphilic substances in posthypertonic shock conditions was studied. For this, the level of hemolysis in posthypertonic shock of native and ATP-depleted erythrocytes in the presence of amphiphilic substances was determined. It was shown that ATP depletion reduces posthypertonic cell damage by 15-17% both at 0°С and at 37°С. It was found that amphiphilic compounds are able to protect rabbit erythrocytes in conditions of posthypertonic shock, but only at a temperature of 0°С. In conditions of ATP-depletion of cells, only anionic sodium decyl sulfate and nonionic decyl-β,D-glucopyranoside retain their protective effect in posthypertonic shock condi- tions, whereas cationic chlorpromazine and trifluoroperazine lose this ability. This can be explained based on the features of the incorporation of amphiphiles into the erythrocyte membrane. All studied amphiphilic compounds increase the level of damage in both control and ATP-depleted cells under conditions of posthypertonic shock at a temperature of 37°C. Probably, easier intercalation and increased fluidity of the membrane at this temperature destabilize the bilayer and reduce the protective effect of amphiphilic compounds.

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Amphiphilic Compounds as Antihemolytic Agents: Problems and Prospects

Chabanenko,

Yershova,

Orlova

et al. 2024

Probl Cryobiol Cryomed

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In this research the effect of surface-active substances belonging to different classes of amphiphilic compounds (cationic trifluoperazine (TFP), anionic sodium decyl sulfate (C10) and nonionic decyl-β,D-glucopyranoside (DGP)) on the level of damage of human erythrocytes during posthypertonic shock (PHS) and glycerol removal from cells frozen to –196°C was study. All amphiphilic compounds in low concentrations (at which the antihemolytic activity is approximately 45% under the conditions of PHS cells) show the same efficiency when removing glycerol from thawed cells. It was established that among amphiphilic compounds in effective concentrations the maximum antihemolytic (AHmax) activity was shown by DGP (74%) during the removal of glycerol from thawed cells and C10 (74%) under conditions of PHS of erythrocytes, while the AHmax activity indicators of TFP were comparable in both cases. A feature of the effectiveness of C10 under the conditions of deglycerolization of cryopreserved erythrocytes is almost the same AH when used in both concentrations. The method of flow cytometry showed that the amount of annexin-labeled cells depended on the concentration of C10 both in physiological solution and under PHS conditions.

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Effect of Trifluoroperazine and Sodium Decyl Sulfate on Posthypertensive Shock of Human and Rabbit Erythrocytes

Cited by 3 publications

References 18 publications

Evaluation of the Form and Distribution of Phosphatidylserine in Human Erythrocytes Exposed to Posthypertonic Shock Under the Protection of Amphiphilic Compounds

Evaluation of the Form and Distribution of Phosphatidylserine in Human Erythrocytes Exposed to Posthypertonic Shock Under the Protection of Amphiphilic Compounds

The Role of Cell Energy Status in the Protection of Posthypertonic Hemolysis by Amphiphilic Substances

Amphiphilic Compounds as Antihemolytic Agents: Problems and Prospects

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