Effect of Trimetazidine on Retinal Ischemia/Reperfusion Injury in Rats

Abstract

Purpose: To investigate the effect of trimetazidine (TMZ), an antioxidant agent, on the ischemia/reperfusion (I/R) injury in rat retina histopathologically. Methods: The retinal I/R model was carried out by the 4-vessel occlusion method on Wistar albino rats. Twenty-one rats were divided into 7 groups, each comprising 3 rats. The animals in groups 1, 2 and 3 underwent 30 min of ischemia + 4 h of reperfusion and were treated by the administration of saline, TMZ before reperfusion and TMZ before ischemia, respectively. The animals in groups 4, 5 and 6 underwent 90 min of ischemia + 4 h of reperfusion and were treated in the same way as those in groups 1, 2 and 3, respectively. The 7th group was sham operated. Results: Thirty and 90 min of ischemia followed by 4 h of reperfusion induced retinal injury in the rat retina. Histopathologically, the inner plexiform and inner nuclear layers were the most affected parts. TMZ was able to reduce almost all retinal I/R damage when administered before ischemia. A cytoprotective effect of TMZ was partly observed in those animals which were treated before reperfusion. Conclusion: TMZ seemed to have a protective effect against retinal I/R injury in rats.

Changes in Retinal Morphology, Electroretinogram and Visual Behavior after Transient Global Ischemia in Adult Rats

“…Compared to the retinal ischemic models mentioned above, the 4-VO model has been employed less to observe the changes in pathology of the retina. Ozden, S. et al reported that transient global ischemia for a duration of 30 to 90 min induced the loss of ganglion cells and amacrine cells [55]. Our study showed that an approximate 17 min duration of transient global ischemia could induce changes in ERG recording and synthesis of protein of ganglion cells and amacrine cells, and anxiety-like behavior but not cell loss.…”

Protective Effect of Trimetazidine in a Model of Ischemia-Reperfusion in the Rat Retina

“…TMZ might be considered a potential therapeutic modality for combating retinal ischemia. Ozden et al [21] have shown, in a recent work, a protective effect of TMZ on the rat retina after ischemia.…”

Protective effects of various antioxidants during ischemia-reperfusion in the rat retina

“…These observations suggest that application of exogenous antioxidants may protect the retina from I/R-induced damage. Indeed, beneficial effects of antioxidant application have been demonstrated on retinal organelles [31,32] and isolated/cultured retinal cells [25,37] in vitro, as well as on the retina of experimental animals in vivo [3, 6-8, 24, 48].…”