Effect of triorganotin compounds on membrane permeability

Ortíz, Antonio Gómez; Teruel, José A.; Aranda, Francisco J.

doi:10.1016/j.bbamem.2005.12.005

Cited by 42 publications

(26 citation statements)

References 37 publications

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“…It has been reported that inclusion of up to 50% cholesterol in phosphatidylcholine membranes almost completely inhibited the passive membrane permeation to CF and ions [36], contents leakage induced by environmental toxicants [37], and surfactant-induced leakage [38]. In lipid bilayers, cholesterol affects the lipid chain order of phospholipids and thus modulates the membrane mechanical properties [39,40].…”

Section: Discussionmentioning

confidence: 99%

Permeabilization of biological and artificial membranes by a bacterial dirhamnolipid produced by Pseudomonas aeruginosa

Sánchez

Aranda

Teruel

et al. 2010

Journal of Colloid and Interface Science

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Section: Discussionmentioning

confidence: 99%

Permeabilization of biological and artificial membranes by a bacterial dirhamnolipid produced by Pseudomonas aeruginosa

Sánchez

Aranda

Teruel

et al. 2010

Journal of Colloid and Interface Science

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“…Phenyltins are chemicals with welldocumented biological activity and with the capability to cross the lipid bilayer by passive diffusion [1,11,12]. It has been shown previously that adsorption of such compounds results in the electrostatic surface potential generation which can be detected with the fluorescent probe located at both membrane surfaces.…”

Section: Resultsmentioning

confidence: 99%

A fluorescence method for determining transport of charged compounds across lipid bilayer

Przybyło¹,

Olżyńska²,

Han³

et al. 2007

Biophysical Chemistry

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT3 AbstractThere is a constant need for simple, economical and time-efficient methods which allow evaluating a compound's ability to penetrate the biological membrane, one of the key parameters needed to characterize biologically active compounds. In the paper we propose a new method of a permeability determination. Instead of detecting the compound's concentration directly, we employ an approach in which the membrane interface is labeled with a fluorescein lipid probe; the probe is sensitive to the presence of charged compounds. The fluorescence intensity changes of the dye permanently attached to both sides of a model lipid bilayer are measured. Specifically, the time course of the fluorescence intensity changes following a rapid induction of a nonequilibrium state of the sample allows the evaluation of the membrane permeability for the compound. The method was validated by the determination of the phenyltin compound's transport through the model phosphatidylcholine unilamellar liposome bilayer.

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“…The decrease in cultured heart cell viability could be explained by the induction of apoptosis and/or necrosis. The lipophilic nature of TBT makes it an active membrane toxicant (Ortiz et al 2005). It was demonstrated using flow cytometry and YO-PRO r staining that around 10% of cells are apoptotic and necrotic after only 10 min of treatment with 10 −6 M TBT.…”

Section: Discussionmentioning

confidence: 99%

Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin

Droguet

Devauchelle

Pennec

et al. 2012

Aquat. Living Resour.

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-European Community regulations on chemicals promote alternative methods to test substances presenting potential risks for the environment. In the present work, cultured atrial cells isolated from oyster (Crassostrea gigas) were used as an experimental model to investigate the toxicity of tributyltin (TBT) after short-time exposure at concentrations representative of those that can be measured in seawater, marine sediments and/or bivalves bioaccumulating this pollutant. In vitro and in vivo assays produce values of the same order of magnitude for both animal/cell survival and heart/cardiomyocyte beating rate. The survival rate of whole animals decreased from 10 −6 M TBT after 3 days. For cultured cells, the viability, evaluated using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay, significantly decreased after two days of treatment with 10 −6 M TBT, and after six days with 10 −10 M TBT. The percentage of apoptotic cells, quantified by flow cytometry and YO-PRO r -1 iodide, a nucleic acid stain that only permeates cells that are beginning to undergo apoptosis, increased significantly in these cases. Moreover, intracellular concentration of Ca ++ had increased after 10 min of exposition to 10 −6 M, and could be associated with apoptotic processes. As patch clamp experiments showed that Ca ++ conductance was decreased, intracellular calcium increase could mainly be due to a release from internal stores. The decreases in beating rhythm could be explained by the decrease in adenosine triphosphate (ATP) production revealed by 31 P nuclear magnetic resonance (NMR) spectroscopy and confirmed by the increase of the K ATP channel conductance. The related hyperpolarization and the disturbances of the energetic metabolism were clearly related to the loss of the atrial cell contractility and viability.

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Effect of triorganotin compounds on membrane permeability

Cited by 42 publications

References 37 publications

Permeabilization of biological and artificial membranes by a bacterial dirhamnolipid produced by Pseudomonas aeruginosa

Permeabilization of biological and artificial membranes by a bacterial dirhamnolipid produced by Pseudomonas aeruginosa

A fluorescence method for determining transport of charged compounds across lipid bilayer

Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin

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