Effect of tris(2-carboxyethyl)phosphine and tertiary butyl alcohol on the performance of convection polymerase chain reaction

Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle

“…To maximize RNA release, protect RNA from degradation, and degrade inhibitors, many studies utilized enzymatic and chemical treatments to complement heat lysis [ 45 , 75 ]. Because RT-PCR and isothermal amplification techniques have different tolerance to chemicals such as reducing reagents and chelating reagents [ [76] , [77] , [78] ], different heat lysis methods are compatible with the subsequent RT-PCR ( Fig. 2 , Table S1 ) and isothermal amplification techniques ( Fig.…”