Effect of triton X-100 on the conjugation of tetrahydrocortisone, in vitro

Separation of oestrone UDP-glucuronyltransferase and p-nitrophenol UDP-glucuronyltransferase activities

“…Hepatic microsomal UDP-glucuronosyltransferase (EC 2.4.1.17) catalyses the conjugation of UDPglucuronic acid with numerous classes of chemicals, such as carboxylic acids (Dutton, 1956), amines (Arias, 1961), thiol derivatives (Dutton & Illing, 1972), bilirubin (Schmid et al, 1957;Lathe & Walker, 1958) and certain hormones (Harri et al, 1970;Miller et al,-1973;Fuchs et al, 1977). Since such a broad spectrum of substrates is glucuronidated, numerous studies have suggested a multiplicity of glucuronyltransferases (Dutton & Burchell, 1977).…”