Effect of Trypsin Treatment of In Vitro Fertilized Bovine Embryos on Their Subsequent Survival and Development.

Otoi, Takeshige; Tachikawa, S.; Kondo, S.; Kono, Fumio; Mitsuhiro, Goto; Ogano, Yoshikazu; Suzuki, Takahiro

doi:10.1292/jvms.55.237

Cited by 10 publications

(1 citation statement)

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“…Blastocysts were partially dehydrated at room temperature (22-258C) in three steps by sequential equilibration in T20 (TCM-199 supplemented with 20% FBS) containing 5%, 7.5% and 10% of each cryoprotectant, namely ethylene glycol (EG), dimethyl sulfoxide (DMSO) and in a mixture containing equal percentages of 2.5%, 3.75% and 5% EG and DMSO, respectively, at 10-min intervals (Otoi et al 1993). Sucrose (0.4 M) was added to the final 10% freezing solutions.…”

Section: Controlled Slow Freezingmentioning

confidence: 99%

Open pulled straw vitrification and slow freezing of sheep IVF embryos using different cryoprotectants

Bhat

Sharma

Khan

et al. 2015

Reprod. Fertil. Dev.

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The aim of the present study was to evaluate the post-thaw survival and hatching rates of sheep blastocysts using different cryoprotectants. In Experiment 1, Day 6 sheep embryos were cryopreserved by a slow freezing protocol using 10% ethylene glycol (EG), 10% dimethyl sulfoxide (DMSO) or a mixture of 5% EG and 5% DMSO. Hatching rates were higher in the 10% EG group than in the 10% DMSO or EG + DMSO groups (30% vs 18% and 20%, respectively). In Experiment 2, embryos were cryopreserved by open pulled straw (OPS) vitrification using either 33% EG, 33% DMSO or a mixture of 16.5% EG + 16.5% DMSO. Re-expansion and hatching rates in the EG + DMSO group (79.16% and 52.74%, respectively) were higher than those in the EG group (64.28% and 30.02%, respectively), whereas the outcomes for the DMSO group were the lowest (45.18% and 8.6%, respectively). In Experiment 3, embryos were cryopreserved by OPS vitrification using either 40% EG, 40% DMSO or a mixture of 20% EG + 20% DMSO. Re-expansion and hatching rates were highest in the EG group than in the EG + DMSO and DMSO groups (92.16% vs 76.30% and 55.84% re-expansion, respectively; and 65.78% vs 45.55% and 14.46% hatching, respectively). In conclusion, OPS vitrification was found to be more efficient for cryopreservation of in vitro-developed sheep embryos than traditional freezing.

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Section: Controlled Slow Freezingmentioning

confidence: 99%