Effect of Tween 20 on antibacterial effects of acidic, neutral and alkaline decontaminants on viability of Salmonella on chicken carcasses and survival in waste decontamination fluids

Dikici, Abdullah; Arslan, Ali; Yalçin, Halil; Özdemir, Pınar; Aydın, Işıl; Çalıcıoğlu, Mehmet

doi:10.1016/j.foodcont.2012.07.043

Cited by 12 publications

(11 citation statements)

References 22 publications

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“…Tween 20, as a member of these types of materials, has been introduced in different parts of human life by various industries (Hoffman and Jump, 1986;Dikici et al, 2013). Some in vivo studies showed low toxicity of tween 20, proposing it as a good candidate for application in drug delivery systems as a potential stabilizing agent (Lu et al, 2011;Weiszhár et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Cyto/Genotoxicity Study of Polyoxyethylene (20) Sorbitan Monolaurate (Tween 20)

Eskandani

Hamishehkar

Dolatabadi

2013

DNA and Cell Biology

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Polyoxyethylene (20) sorbitan monolaurate (tween 20) is a non-ionic surfactant that is widely used as an emulsifier and stabilizer in pharmaceutical formulations, food and cosmetic industries. Although a number of studies have showed its non-toxic impacts on target cells, still, it is essential to investigate its effect on target cells. Therefore, in the present study, the anti-cell proliferation and cyto/genotoxicity effects of tween 20 are reported to address the possible mechanism for induction of apoptosis. At 40%-50% confluency, A549 cells and human umbilical vein endothelial cells were exposed to tween 20 at a recommended concentration for 24 h. After 24 h, to detect apoptosis and DNA damage, the treated cells were subjected to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, fluorescein isothiocyanate (FITC)-labeled annexin V flow cytometry, DAPI staining, comet, and DNA ladder assays. Tween 20 decreased the growth of treated cells dose and time dependently, and single-strand DNA cleavage has been confirmed by comet assay. In addition, morphological alteration of DAPI-stained cells showed clear fragmentation in the chromatin and DNA rings within the nucleus of tween 20-treated cells. In addition, flow cytometry and DNA fragmentation assays confirmed DAPI staining assay results and indicated the occurrence of a programmed cell death (apoptosis) in the treated cells. These results demonstrate that, despite consideration of tween 20 as a safe non-ionic surfactant, it can induce apoptosis in target cells.

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Section: Discussionmentioning

confidence: 99%

Cyto/Genotoxicity Study of Polyoxyethylene (20) Sorbitan Monolaurate (Tween 20)

Eskandani

Hamishehkar

Dolatabadi

2013

DNA and Cell Biology

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“…Slaughter process of a chicken may take 3-4 h (the period from scalding tank to packaging), and bacterial contamination may occur at any time of the production process. On the other hand, many decontamination studies have been conducted to eliminate or reduce the foodborne pathogens on chicken meat, and in those studies the contact times that were given to bacteria for the attachment to meat surface are highly variable (changing from 2 to 60 min) (Dikici et al 2013;Lee et al 2014;Karuppasamy et al 2015). It has been reported that Salmonella cells that firmly attached to poultry skin are significantly more resistant to chemical interventions (Mikolajczyk 2015).…”

Section: Introductionmentioning

confidence: 99%

Effect of some chemical decontaminants on the survival of Listeria monocytogenes and Salmonella Typhimurium with different attachment times on chicken drumstick and breast meat

2018

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The aim of this study was to investigate the efficacy of some decontaminant agents on the survival of Typhimurium and with different attachment periods to chicken drumstick with skin and skinless breast meat. For this purpose, Typhimurium and were given periods of 0.5 (30 s), 20 and 210 min to attach to the chicken drumstick and breast meat. At the end of the each attachment period, the meat samples were treated with lactic acid, (2 and 4%), cetylpyridinium chloride (0.5%) and acidified sodium chlorite (1200 ppm). In the drumstick sample treated with cetylpyridinium chloride, the reduction level of with 30 s attachment period was 3.2 log CFU/ml while the reduction level was found to be 2.2 log CFU/ml with 20 min attachment period. Decontamination with acidified sodium chlorite resulted in reduction of 1.8 log CFU/ml in Typhimurium attached to the chicken drumstick for 30 s while the reduction levels of Typhimurium with 20 and 210 min attachment periods were 1.2 and 1.3 log CFU/ml, respectively. The results indicated that some antimicrobial agents have more strong effect on and Typhimurium on the chicken meat parts in the first 30 s of attachment. However, there were no changes in the efficacy of the decontaminants on the survival of and Typhimurium on chicken meat when the attachment time of these bacteria were extended from 20 min to 210 min.

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“…Lactic acid, cetyl pyridinium chloride and trisodium phosphate immersion treatments all were all able to significantly reduce Salmonella on carcasses relative to tap water immersion, by 2.4-4.8 log10 CFU/mL carcass rinsate [409]. Combination of these treatments with the surfactant Tween 20 did not lead to a further reduction [409].…”

Section: Organic Acidmentioning

confidence: 90%

“…Post evisceration spray or immersion treatment with a citric and lactic acid mixture can reduce Salmonella on carcasses by 1.3 and 2.3 log10 CFU/mL carcass rinsate respectively [401]. Lactic acid, cetyl pyridinium chloride and trisodium phosphate immersion treatments all were all able to significantly reduce Salmonella on carcasses relative to tap water immersion, by 2.4-4.8 log10 CFU/mL carcass rinsate [409]. Combination of these treatments with the surfactant Tween 20 did not lead to a further reduction [409].…”

Section: Organic Acidmentioning

confidence: 98%

Advances in Enteropathogen Control Throughout the Meat Chicken Production Chain

Stewart¹,

Pavic²

2022

Preprint

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Enteropathogens, namely Salmonella and Campylobacter, are a concern in global public health and have been attributed in numerous risk assessments to a poultry source. During the last decade a large body of research addressing this problem has been published. The literature reviewed contains review articles on certain aspects of poultry production chain, however in the past decade there hasn’t been a review on the through production chain, farm to fork, production of poultry. This review, a pool of 514 articles were selected for relevance via a systematic screening process (from >7500 original search articles). These studies identified a diversity of management and intervention strategies for the elimination or reduction of enteropathogens in poultry production. Many studies were laboratory or limited field trials with implementation in true commercial operations being problematic. Entities considering using commercial anti-enteropathogen products and interventions are advised to perform an internal validation and fit for purpose trial as Salmonella and Campylobacter serovars and biovars may have regional diversity. Future research should focus on non-chemical application within the processing plant and how synergistic through chain intervention may contribute to reducing the overall carcass burden of enteropathogen, coupled with increased consumer education on safe handling and cooking of poultry.

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Effect of Tween 20 on antibacterial effects of acidic, neutral and alkaline decontaminants on viability of Salmonella on chicken carcasses and survival in waste decontamination fluids

Cited by 12 publications

References 22 publications

Cyto/Genotoxicity Study of Polyoxyethylene (20) Sorbitan Monolaurate (Tween 20)

Cyto/Genotoxicity Study of Polyoxyethylene (20) Sorbitan Monolaurate (Tween 20)

Effect of some chemical decontaminants on the survival of Listeria monocytogenes and Salmonella Typhimurium with different attachment times on chicken drumstick and breast meat

Advances in Enteropathogen Control Throughout the Meat Chicken Production Chain

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