Effect of Two Biological Formulations Based onBacillus subtilisandPseudomonas fluorescenson Control ofDidymella applanata, the Causal Agent of Red Raspberry Cane Spur Blight

Штерншис, М. В.; Shpatova, T.V.; Belyaev, A. A.

doi:10.1155/2016/2797125

Cited by 11 publications

(6 citation statements)

References 30 publications

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“…Several Pseudomonas ASVs were more prevalent earlier in fruit ripening and others later. Pseudomonas species have varying roles, with some P. syringae strains pathogenic to raspberry (Ivanović et al, 2023) and P. fluorescens as a biological control agent against Didymella applanate (spur blight) on raspberry (Shternshis et al, 2016). Epiccocum and Rouxiella spp.…”

Section: Discussionmentioning

confidence: 99%

“…were more prevalent at the later fruit development stages, and Bacillus more prevalent on green fruit. All these genera contain species with strains that have been tested as biocontrol organisms against raspberry pathogens (Christova & Slavov, 2021; Shternshis et al, 2016). Strains in the Bacillus genus have been frequently used in commercial biopesticide development on soft fruits.…”

Section: Discussionmentioning

confidence: 99%

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Investigating the inoculum dynamics of Cladosporium on the surface of raspberry fruits and in the air

Farwell,

Papp‐Rupar,

Deakin

et al. 2024

Environmental Microbiology

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Raspberry production is under threat from the emerging fungal pathogenic genus Cladosporium. We used amplicon‐sequencing, coupled with qPCR, to investigate how fruit age, fruit location within a polytunnel, polytunnel location and sampling date affected the fruit epiphytic microbiome. Fruit age was the most important factor impacting the fungal microbiome, followed by sampling date and polytunnel location. In contrast, polytunnel location and fruit age were important factors impacting the bacterial microbiome composition, followed by the sampling date. The within‐tunnel location had a small significant effect on the fungal microbiome and no effect on the bacterial microbiome. As fruit ripened, fungal diversity increased and the bacterial diversity decreased. Cladosporium was the most abundant fungus of the fruit epiphytic microbiome, accounting for nearly 44% of all fungal sequences. Rotorod air samplers were used to study how the concentration of airborne Cladosporium inoculum (quantified by qPCR) varied between location (inside and outside the polytunnel) and time (daytime vs. nighttime). Quantified Cladosporium DNA was significantly higher during the day than the night and inside the polytunnel than the outside. This study demonstrated the dynamic nature of epiphytic raspberry fruit microbiomes and airborne Cladosporium inoculum within polytunnels, which will impact disease risks on raspberry fruit.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Investigating the inoculum dynamics of Cladosporium on the surface of raspberry fruits and in the air

Farwell,

Papp‐Rupar,

Deakin

et al. 2024

Environmental Microbiology

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“…Last but not least, there are bacteria representatives belonging to the Pseudomonas genus used in commercially available bioproducts [ 6 ]. Pseudomonas strains were found to be effective in decreasing severity of red raspberry cane spur blight [ 41 ]. It has been proven that Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional Characterization

Pylak

Oszust

Frąc

2020

IJMS

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The threat caused by plants fungal and fungal-like pathogens is a serious problem in the organic farming of soft fruits. The European Commission regulations prohibit some commercially available chemical plant protection products, and instead recommend the use of natural methods for improving the microbial soil status and thus increasing resistance to biotic stresses caused by phytopathogens. The solution to this problem may be biopreparations based on, e.g., bacteria, especially those isolated from native local environments. To select proper bacterial candidates for biopreparation, research was provided to preliminarily ensure that those isolates are able not only to inhibit the growth of pathogens, but also to be metabolically effective. In the presented research sixty-five isolates were acquired and identified. Potentially pathogenic isolates were excluded from further research, and beneficial bacterial isolates were tested against the following plant pathogens: Botrytis spp., Colletotrichum spp., Phytophthora spp., and Verticillium spp. The eight most effective antagonists belonging to Arthrobacter, Bacillus, Pseudomonas, and Rhodococcus genera were subjected to metabolic and enzymatic analyses and a resistance to chemical stress survey, indicating to their potential as components of biopreparations for agroecology.

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“…B. velezensis BZR 336 g and B. velezensis BZR 517 strains were used at concentrations of 10 4 -10 6 CFU/mL. The experiment was conducted using the agar block techniques (Shternshis, Shpatova & Belyaev, 2016).…”

Section: In Vitro Antifungal Activity Of Bacillus Strainsmentioning

confidence: 99%

Biological control of important fungal diseases of potato and raspberry by two Bacillus velezensis strains

Асатурова

Штерншис

Tsvetkova

et al. 2021

PeerJ

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Stem canker and black scurf caused by Rhizoctonia solani are the important diseases in potato, while spur blight caused by Didymella applanata is a major disease in red raspberry. In Western Siberia, both crops are grown predominantly in small-scale farming that requires maximal usage of biological products for plant protection instead of chemicals. We evaluated two promising Bacillus velezensis strains BZR 336 g and BZR 517 isolated in the south of Russia (45°1′N, 38°59′E) for their biological control potentials against the potato and red raspberry diseases under the more severe weather conditions of Western Siberia (55°1′N, 82°55′ E). We tested two techniques to apply biocontrol agents: (1) coating the seeds (potato tubers) and (2) spraying over the plants (raspberry canes). In each case, we estimated B. velezensis strains on two plant cultivars differed by the disease resistance. The degree of B. velezensis influence on disease incidence and severity depended on the bacterial strain, the protected plant, and its cultivar. We also demonstrated that two B. velezensis strains significantly stimulated plant growth of potato, which contributed to the plant productivity on both cultivars. The BZR 336 g strain affected the potato productivity more than the BZR 517 strain. Under the influence of both bacterial strains, raspberry yield was significantly higher compared to the control on the susceptible cultivar. These findings indicated that two southern B. velezensis strains had proved their efficacy as biological control agents in the control of the serious fungal infection of potato and raspberry plants under the more severe ecological conditions of Western Siberia. For the first time, we demonstrated B. velezensis strains potential for use as biological control agents against R. solani on potato, and against D. applanata on red raspberry.

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Effect of Two Biological Formulations Based onBacillus subtilisandPseudomonas fluorescenson Control ofDidymella applanata, the Causal Agent of Red Raspberry Cane Spur Blight

Cited by 11 publications

References 30 publications

Investigating the inoculum dynamics of Cladosporium on the surface of raspberry fruits and in the air

Investigating the inoculum dynamics of Cladosporium on the surface of raspberry fruits and in the air

Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional Characterization

Biological control of important fungal diseases of potato and raspberry by two Bacillus velezensis strains

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